Results 11 to 20 of about 16,112 (276)
Functional indications for transposase domestications – Characterization of the human piggyBac transposase derived (PGBD) activities [PDF]
Gene, 2022 Transposable elements are widespread in all living organisms. In addition to self-reproduction, they are a major source of genetic variation that drives genome evolution but our knowledge of the functions of human genes derived from transposases is limited.
Kolacsek, Orsolya +10 more
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Bacterial transposases and retroviral integrases [PDF]
Molecular Microbiology, 1995 SummaryTransposable genetic elements have adopted two major strategies for their displacement from one site to another within and between genomes. One involves passage through an RNA intermediate prior to synthesis of a DNA copy while the other is limited uniquely to DNA intermediates.
P, Polard, M, Chandler
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Tn5 Transposase Applied in Genomics Research [PDF]
International Journal of Molecular Sciences, 2020 The development of high-throughput sequencing (next-generation sequencing technology (NGS)) and the continuous increase in experimental throughput require the upstream sample processing steps of NGS to be as simple as possible to improve the efficiency of the entire NGS process.
Li, Niannian +5 more
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Tn 5 Transposase Active Site Mutants [PDF]
Journal of Biological Chemistry, 2002 Tn5 transposase (Tnp) is a 53.3-kDa protein that is encoded by and facilitates movement of transposon Tn5. Tnp monomers contain a single active site that is responsible for catalyzing a series of four DNA breaking/joining reactions at one transposon end.
Todd A, Naumann, William S, Reznikoff
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Transposon domestication versus mutualism in ciliate genome rearrangements. [PDF]
PLoS Genetics, 2013 Ciliated protists rearrange their genomes dramatically during nuclear development via chromosome fragmentation and DNA deletion to produce a trimmer and highly reorganized somatic genome.
Alexander Vogt +3 more
doaj +1 more source
Functional Traits Co-Occurring with Mobile Genetic Elements in the Microbiome of the Atacama Desert [PDF]
, 2019 Mobile genetic elements (MGEs) play an essential role in bacterial adaptation and evolution. These elements are enriched within bacterial communities from extreme environments. However, very little is known if specific genes co-occur with MGEs in extreme
Airo, Alessandro +4 more
core +1 more source
Prevalence of Antibiotic Resistome in Ready-to-Eat Salad [PDF]
, 2020 Ready-to-eat salad harbors microorganisms that may carry various antibiotic resistance genes (ARGs). However, few studies have focused on the prevalence of ARGs on salad, thus underestimating the risk of ARGs transferring from salad to consumers. In this
Giles, Madeline +7 more
core +2 more sources
Microorganisms, 2019 A high level of transposon-mediated genome rearrangement is a common trait among microorganisms isolated from thermal environments, probably contributing to the extraordinary genomic plasticity and horizontal gene transfer (HGT) observed in these ...
Alba Blesa +5 more
doaj +1 more source
Escherichia coli EHEC Germany outbreak preliminary functional annotation using BG7 system [PDF]
, 2011 We have annotated the European outbreak E. coli EHEC genome sequenced by BGI (6-2-2011) and assembled with MIRA by Nick Loman (6-2-2011 ). Our system BG7, Bacterial Genome annotation of Era7 Bioinformatics, predicts ORFs and annotates them based on ...
Eduardo Pareja +4 more
core +2 more sources
RAG-1 Mutations Associated with B-Cell-Negative SCID Dissociate the Nicking and Transesterification Steps of V(D)J Recombination [PDF]
, 2001 Some patients with B-cell-negative severe combined immune deficiency (SCID) carry mutations in RAG-1 or RAG-2 that impair V(D)J recombination. Two recessive RAG-1 mutations responsible for B-cell-negative SCID, R621H and E719K, impair V(D)J recombination
Chang, Fu-Chung +2 more
core +2 more sources