Results 71 to 80 of about 10,355 (255)
BMC Biotechnology, 2011 Background DNA transposons have emerged as indispensible tools for manipulating vertebrate genomes with applications ranging from insertional mutagenesis and transgenesis to gene therapy.
Yu Robert K +5 more
doaj +1 more source
Transposase‐Assisted Donor Tethering Boosts Large‐Fragment HDR in Plants
Advanced Science, EarlyView.A transposase‐assisted donor tethering strategy is developed to enhance homology‐directed repair in plants. By recruiting donor DNA to double‐strand breaks and synergizing with repair pathway reprogramming and transcription‐coupled donor design, this system markedly improves large‐fragment targeted insertion efficiency, providing a robust platform for ...
Sha Wei +8 more
wiley +1 more source
Autoregulation of phage μ transposase at the level of translation [PDF]
Nucleic Acids Research, 1988 The bacteriophage Mu A and B genes, which lie adjacent to each other and are colinear on the phage genome, encode proteins that catalyze efficient transposition of Mu DNA. We show that the molar ratio of A and B proteins is approximately 1:20 in extracts prepared after induction of cells containing a Mu lysogen or a plasmid carrying the Mu fragment ...
R L, Parsons, R, Harshey
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Advanced Science, EarlyView.IGF2BP1‐mediated m6A stabilization sustains SMC1A expression, enabling cohesin‐associated chromatin regulation of Nestin in hepatocellular carcinoma. This work reveals an epitranscriptomic‐chromatin‐cytoskeletal regulatory axis linked to malignant phenotypes and identifies SMC1A as a biologically relevant vulnerability in HCC.
Zhenxiang Peng +7 more
wiley +1 more source
Transposition without transposase: a spontaneous mutation in bacteria [PDF]
Journal of Bacteriology, 1997 Transposition mutations are typically associated with the activities of transposable elements such as transposons and insertion sequences, whose mobility is dependent upon transposase enzymes that catalyze exchanges between element ends and target sites. We describe a single transposition event in which a block of donor sequence is inserted at a target
C A, Rappleye, J R, Roth
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Advanced Science, EarlyView.This study identifies the mechanosensor PIEZO1 as a key factor in promoting acute myeloid leukemia progression and uncovers a ferroptosis defense system mediated by PIEZO1‐HIF1A‐SLC7A11 axis that is essential for leukemia stem cell maintenance. This ferroptotic defense system may represent a unique vulnerability for leukemia stem cells, providing a ...
Tiantian Zhang +10 more
wiley +1 more source
Cell & Bioscience, 2011 Background Laribacter hongkongensis is associated with community-acquired gastroenteritis and traveler's diarrhea. In this study, we performed an in-depth annotation of the genes in its genome related to the various steps in the infective process, drug ...
Lau Susanna KP +7 more
doaj +1 more source
Advanced Science, EarlyView.P3FI–90 treatment targets KDM3B, reshapes the epigenetic landscape, and suppresses SHP1 expression, thereby activating STING–TBK1–IRF3–type I IFN signaling pathway. Consequently, CD8+ T cells are recruited to the tumor site and activated to produce IFN–γ and GZMB, leading to the killing of TNBC cells.
Xiaolong Wang +8 more
wiley +1 more source
Advanced Science, EarlyView.We developed the ASCAL pipeline, integrating complementary spatial transcriptomics, to construct a high‐fidelity mouse whole‐eye single‐cell atlas. Applying ASCAL to a retinal artery occlusion (RAO) model revealed spatially restricted immune activation localized to the ganglion cell layer and the selective depletion of a translationally active, outer ...
Chen Du +11 more
wiley +1 more source
Increasing transposase abundance with ocean depth correlates with a particle-associated lifestyle
mSystemsTransposases are mobile genetic elements that move within and between genomes, promoting genomic plasticity in microorganisms. In marine microbial communities, the abundance of transposases increases with depth, but the reasons behind this trend remain ...
Juntao Zhong +5 more
doaj +1 more source