Results 81 to 90 of about 17,057 (320)
Tn 5 Transposase Active Site Mutants [PDF]
Journal of Biological Chemistry, 2002 Tn5 transposase (Tnp) is a 53.3-kDa protein that is encoded by and facilitates movement of transposon Tn5. Tnp monomers contain a single active site that is responsible for catalyzing a series of four DNA breaking/joining reactions at one transposon end.
Todd A, Naumann, William S, Reznikoff
openaire +2 more sources
Development of a papillation assay using constitutive promoters to find hyperactive transposases
, 2018 Background Transposable elements is an extremely diverse group of genetic elements encoding their own mobility. This ability has been exploited as a powerful tool for molecular biology and genomics techniques. However, transposition activity is regulated
Michael Tellier, R. Chalmers
semanticscholar +1 more source
Structural Basis for the Inverted Repeat Preferences of mariner Transposases
Journal of Biological Chemistry, 2015 Background: Transposases orchestrate movement of DNA transposons around and between genomes. Results: Structural and biochemical approaches are combined to dissect the DNA preferences of two mariner transposases in each step of transposition. Conclusion:
M. Trubitsyna +4 more
semanticscholar +1 more source
Advanced Science, EarlyView.Lung cancer remains the leading cause of cancer‐related death. We investigated the role of the epigenetic regulator DMAP1 in NSCLC and found that its loss induces replication stress and DNA damage. This in turn activates type I IFN signaling via the cGAS–STING pathway and transcriptional ISG de‐repression, enhancing anti‐tumor immune responses ...
Kan Huang +10 more
wiley +1 more source
A Transposon and Transposase System for Human Application
Molecular Therapy, 2010 The stable introduction of therapeutic transgenes into human cells can be accomplished using viral and nonviral approaches. Transduction with clinical-grade recombinant viruses offers the potential of efficient gene transfer into primary cells and has a record of therapeutic successes.
Hackett, Perry B +2 more
openaire +2 more sources
Biochemical Characterization and Comparison of Two Closely Related Active mariner Transposases
Biochemistry, 2014 Most DNA transposons move from one genomic location to another by a cut-and-paste mechanism and are useful tools for genomic manipulations. Short inverted repeat (IR) DNA sequences marking each end of the transposon are recognized by a DNA transposase ...
M. Trubitsyna +3 more
semanticscholar +1 more source
Advanced Science, EarlyView.Integrated multi‐omic profiling maps the gene‐regulatory landscape of the coelomic mesothelium across heart, lung, and pancreas. A cardiac‐restricted regulatory program is uncovered in which TBX20 activates heart mesothelial (epicardial) cis‐regulatory elements, while MAF emerges as a conserved regulator of mesothelial identity.
Quang Minh Dang +3 more
wiley +1 more source
Mobile DNA, 2020 Background Transposable elements (TEs) are a diverse group of self-mobilizing DNA elements. Transposition has been exploited as a powerful tool for molecular biology and genomics.
Michael Tellier, Ronald Chalmers
doaj +1 more source
Microbiology Spectrum, 2022 Streptomyces rimosus ATCC 10970 is the parental strain of industrial strains used for the commercial production of the important antibiotic oxytetracycline.
Lucija Slemc +21 more
doaj +1 more source
Excision efficiency is not strongly coupled to transgenic rate: cell type dependent transposition efficiency of Sleeping Beauty and piggyBac DNA transposons [PDF]
, 2014 The Sleeping Beauty (SB) and piggyBac (PB) DNA transposons represent an emerging new gene delivery technology, potentially suitable for human gene therapy applications.
Apáti, Ágota +7 more
core +1 more source