Results 81 to 90 of about 16,112 (276)

Transposase mediated construction of RNA-seq libraries [PDF]

Genome Research, 2011
RNA-seq has been widely adopted as a gene-expression measurement tool due to the detail, resolution, and sensitivity of transcript characterization that the technique provides. Here we present two transposon-based methods that efficiently construct high-quality RNA-seq libraries.
Jason, Gertz, Katherine E, Varley, Nicholas S, Davis, Bradley J, Baas, Igor Y, Goryshin, Ramesh, Vaidyanathan, Scott, Kuersten, Richard M, Myers   +7 more
openaire   +2 more sources

Multicohort Validation of Gut Microbiome Signatures for Cholangiocarcinoma Diagnosis and Functional Characterization of Bifidobacterium Pseudocatenulatum

Advanced Science, EarlyView.
This study analyzes gut bacteria in cholangiocarcinoma patients, revealing distinct microbial signatures that enable accurate disease detection. Species‐based diagnostic models achieved over 98% accuracy in identifying cholangiocarcinoma and distinguished it from other liver diseases. The research demonstrates that specific beneficial bacteria suppress
Benchen Rao, Jianwen Jiang, Ruiqing Zhang, Dongya Zhang, Chenchen Zhang, Ang Li, Haifeng Lu, Hua Zhang, Lin Zhou, Wenzhi Guo, Peihao Wen, Jianfeng Xue, Jie Pan, Tuerganaili Aji, Zhou Lan, Xianzhi Jiang, Shusen Zheng, Zujiang Yu, Zhigang Ren   +18 more
wiley   +1 more source

Excision efficiency is not strongly coupled to transgenic rate: cell type dependent transposition efficiency of Sleeping Beauty and piggyBac DNA transposons [PDF]

, 2014
The Sleeping Beauty (SB) and piggyBac (PB) DNA transposons represent an emerging new gene delivery technology, potentially suitable for human gene therapy applications.
Apáti, Ágota, Erdei, Zsuzsa, Ivics, Zoltán, Izsvák, Zsuzsanna, Kolacsek, Orsolya, Orbán, Tamás I., Sarkadi, Balázs, Sándor, Sára   +7 more
core   +1 more source

Gut Lactate Boosts Ruminococcus via Histone Lactylation to Mediate Time‐Restricted Feeding Protection in Crohn's Disease

Advanced Science, EarlyView.
TRF enriches Ruminococcus, a bacterial genus producing SCFAs, and activates the epithelial HIF‐1α signaling pathway. This mechanism protects the colonic mucosa from inflammatory insults in colitis models. Mechanistically, gut lactate production during starvation and refeeding mediates H4K12la, which increases SLC9A3 expression and creates an acidic gut
Linwen Huang, Huishi Tan, Senhui Weng, Yuntao Liu, Lingxu Song, Shaoyu Cheng, Zelong Lin, Jiawei Chen, Fei Tan, Jun Wang, Jinke Huang, Linkun Cai, Jiwei Chai, Cailing Zhong, Yanqiang Shi, Wendi Zhang, Haiyan Zhang, Chongyang Huang   +17 more
wiley   +1 more source

Genome-wide target profiling of piggyBac and Tol2 in HEK 293: pros and cons for gene discovery and gene therapy

BMC Biotechnology, 2011
Background DNA transposons have emerged as indispensible tools for manipulating vertebrate genomes with applications ranging from insertional mutagenesis and transgenesis to gene therapy.
Yu Robert K, Chung Pei-Cheng, Yang Herng-Shing, Weirauch Matthew T, Meir Yaa-Jyuhn J, Wu Sareina C-Y   +5 more
doaj   +1 more source

Genomic analysis of 48 paenibacillus larvae bacteriophages [PDF]

, 2018
Indexación: Scopus.Funding: Research at UNLV was funded by National Institute of General Medical Sciences grant GM103440 (NV INBRE), the UNLV School of Life Sciences, and the UNLV College of Sciences. E.C.-N.
Castro-Nallar, E., Fajardo, C.P., Grose, J.H., Hope, S., Mendez, K.N., Stamereilers, C., Tsourkas, P.K., Walker, J.K.   +7 more
core   +1 more source

Targeting m6A Reader YTHDF1 Enhances Antitumor Immunity and Potentiates Anti‐PD‐L1 Efficacy in Intrahepatic Cholangiocarcinoma

Advanced Science, EarlyView.
The m6A reader YTHDF1 drives intrahepatic cholangiocarcinoma (ICC) progression by remodeling the tumor immune microenvironment. YTHDF1 promotes MDSC recruitment via activation of m6A‐FOSL2‐CXCL6/CXCR2 axis, thereby suppressing CD8+ T cell infiltration and function.
Li Luo, Ziqin Liu, Zimin Song, Dong Zhang, Cong Liang, Fei Fang, Kai Lei, Lina Wang, Weikang Chen, Shunli Shen, Ming Kuang, Xiaoxing Li, Jun Yu, Shiyan Wang, Lixia Xu   +14 more
wiley   +1 more source

DNA transposons have colonized the genome of the giant virus Pandoravirus salinus [PDF]

, 2015
BACKGROUND: Transposable elements are mobile DNA sequences that are widely distributed in prokaryotic and eukaryotic genomes, where they represent a major force in genome evolution.
Cheng Sun, Cédric Feschotte, Rachel Lockridge Mueller, Zhiqiang Wu   +3 more
core   +1 more source

A hyperactive piggyBac transposase for mammalian applications [PDF]

Proceedings of the National Academy of Sciences, 2011
DNA transposons have been widely used for transgenesis and insertional mutagenesis in various organisms. Among the transposons active in mammalian cells, the moth-derived transposon piggyBac is most promising with its highly efficient transposition, large cargo capacity, and precise repair of
Yusa K, Zhou L, Li MA, Bradley A, Craig NL   +4 more
openaire   +2 more sources

Genetic Control of Tissue Remodeling by a Non‐Coding SNP in ITGA8 Explains Carotenoid‐Based Color Polymorphism in Marine Mollusks

Advanced Science, EarlyView.
In this study, the orange‐muscle giant abalone (Haliotis gigantea) is used as a model to identify a non‐coding SNP that disrupts the interaction between ITGA8 pre‐mRNA and the splicing factor ILF2, leading to altered ITGA8 splicing. These splicing changes promote carotenoid accumulation in abalone muscle through the regulation of tissue remodeling ...
Xiaohui Wei, Wenzhu Peng, Zekun Huang, Bingye Yang, Yiyu Wu, Minzhen Han, Yi Wang, Bingling Peng, Guosheng Hu, Jiawei Hong, Guijia Liu, Linwei Nie, Yang Gan, Miaoqin Huang, Xuan Luo, Weiwei You, Wen Liu, Caihuan Ke   +17 more
wiley   +1 more source