Results 61 to 70 of about 11,423 (138)

Handmade Biopsy for Genotyping of In Vitro‐Produced Bovine Embryos

Anatomia, Histologia, Embryologia, Volume 55, Issue 3, May 2026.
ABSTRACT Blastocyst biopsy allows genomic selection of pre‐implantation bovine embryos, reducing generation interval and enhancing the genetic gain. We aimed to evaluate the suitability of a simple biopsy procedure, performed by hands, using a splitting microblade and a stereoscope, on in vitro‐produced embryos. Crossbred Bos taurus x B.
Luiz Sergio Almeida Camargo, Carolina Capobiango Romano Quintão, Julia Medeiros Cruz Lima de Martins, Marcos Vinicius Gualberto Barbosa Silva, Agostinho Jorge Reis dos Camargo, Celio Freitas, Daniele Ribeiro Lima de Reis, Marco Antonio Machado, Clara Slade Oliveira   +8 more
wiley   +1 more source

Mosaicism in preimplantation human embryos

Интегративная физиология, 2020
Since the very first publications on preimplantation genetic testing, researchers have faced a serious problem — a high mosaicism level in the preimplantation human embryos obtained by means of in vitro fertilization cycles.
Алсу Фаритовна Сайфитдинова, Олег Сергеевич Глотов, Ирина Васильевна Полякова, Наталья Константиновна Бичевая, Юлия Артемьевна Логинова, Рена Алескеровна Кузнецова, Ольга Анатольевна Леонтьева, Елизавета Евгеньевна Невская, Ольга Андреевна Павлова, Ирина Леонидовна Пуппо, Антон Евгеньевич Шиков, Станислав Петрович Уразов, Татьяна Владимировна Кузнецова, Андрей Михайлович Сарана, Сергей Григорьевич Щербак, Владислав Сергеевич Баранов   +15 more
doaj   +1 more source

insideOutside: an accessible algorithm for classifying interior and exterior points, with applications in embryology

Biology Open, 2023
Stanley E. Strawbridge, Agata Kurowski, Elena Corujo-Simon, Alastair N. Fletcher, Jennifer Nichols, Alexander G. Fletcher   +5 more
doaj   +1 more source

Trophectoderm, Inner Cell Mass, and Expansion Status for Live Birth Prediction After Frozen Blastocyst Transfer: The Winner Is Trophectoderm

Life
Despite advancements in technologies such as time-lapse microscopy and artificial intelligence, the gold standard for embryo selection still relies on standard morphological assessment. Several studies have investigated the correlation between blastocyst
Alessandro Bartolacci, Sofia de Girolamo, Lisett Solano Narduche, Elisa Rabellotti, Lucia De Santis, Enrico Papaleo, Luca Pagliardini   +6 more
doaj   +1 more source

Super-enhancer-guided mapping of regulatory networks controlling mouse trophoblast stem cells

Nature Communications, 2019
Trophectoderm lineage development is essential for implantation, placentation, and healthy pregnancy. Here the authors map super-enhancers (SEs) in trophoblast stem cells and find both TE-specific master regulators and 150 previous uncharacterised ...
Bum-Kyu Lee, Yu jin Jang, Mijeong Kim, Lucy LeBlanc, Catherine Rhee, Jiwoon Lee, Samuel Beck, Wenwen Shen, Jonghwan Kim   +8 more
doaj   +1 more source

Generation of Cdx2-mCherry knock-in murine ES cell line to model trophectoderm and intestinal lineage differentiation

Stem Cell Research, 2019
Caudal-type homeobox 2 (.Cdx2) transcription factor is an essential regulator of differentiation to the intestinal epithelium, somatic mesoderm and trophectoderm function in the mouse.
Debabrata Jana, Hanuman T. Kale, P. Chandra Shekar   +2 more
doaj   +1 more source

Spatial dynamics of mTOR pathway activity during bovine embryo development

Frontiers in Cell and Developmental Biology
The mTOR pathway regulates cell proliferation, growth, survival, and metabolism by integrating nutritional and growth factor signaling. In embryos, its activity is influenced by the availability of nutrients in the culture medium, and it can affect the ...
Francieli P. Berling, Viviane B. G. Bacaro, Ricardo I. de Paschoal, Marcella P. Milazzotto, Marcelo D. Goissis   +4 more
doaj   +1 more source

Complex aneuploidy triggers autophagy and p53-mediated apoptosis and impairs the second lineage segregation in human preimplantation embryos

eLife
About 70% of human cleavage stage embryos show chromosomal mosaicism, falling to 20% in blastocysts. Chromosomally mosaic human blastocysts can implant and lead to healthy new-borns with normal karyotypes.
Marius Regin, Yingnan Lei, Edouard Couvreu De Deckersberg, Charlotte Janssens, Anfien Huyghebaert, Yves Guns, Pieter Verdyck, Greta Verheyen, Hilde Van de Velde, Karen Sermon, Claudia Spits   +10 more
doaj   +1 more source

Non-invasively predicting euploidy in human blastocysts via quantitative 3D morphology measurement: a retrospective cohort study

Reproductive Biology and Endocrinology
Background Blastocyst morphology has been demonstrated to be associated with ploidy status. Existing artificial intelligence models use manual grading or 2D images as the input for euploidy prediction, which suffer from subjectivity from observers and ...
Guanqiao Shan, Khaled Abdalla, Hang Liu, Changsheng Dai, Justin Tan, Junhui Law, Carolyn Steinberg, Ang Li, Iryna Kuznyetsova, Zhuoran Zhang, Clifford Librach, Yu Sun   +11 more
doaj   +1 more source

IAPEz retrotransposons regulate the first lineage segregation in mouse pre-implantation development. [PDF]

Nucleic Acids Res
Huang R, Han Z, Liufu Z, Quan X, Li D, Dong H, Hong G, Chen R, Zhang M, Peng G, Pei D, Chen J, He J.   +12 more
europepmc   +1 more source