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Characterisation of pentamidine-resistant Trypanosoma brucei brucei
Molecular and Biochemical Parasitology, 1995Following selection in vitro by exposure to increasing concentrations of the aromatic diamidine pentamidine, a Trypanosoma brucei brucei clone has been characterised in vivo and in vitro. The resistant clone, designated T.b. brucei S427/118/PR32.6, was found to be less virulent than the parental clone T.b.
Berger, Bradley J. +2 more
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Effect of putrescine on the respiration of Trypanosoma brucei brucei
Molecular and Biochemical Parasitology, 1986When bloodstream forms of Trypanosoma brucei brucei were exposed to exogenous putrescine for 24 h during in vitro culture, the rate of O2 consumption increased significantly in a concentration-related and time-dependent manner. Trypanosomes cultured with 100 microM DL-alpha-difluoromethylornithine (DFMO), an irreversible inhibitor of ornithine ...
B F, Giffin, P P, McCann, C J, Bacchi
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Presence of a lipophosphoglycan in two variants of trypanosoma brucei brucei
Biochemical and Biophysical Research Communications, 1988For the family of Trypanosomatidae (Trypanosoma and Leishmania) the organization of the glycoproteins on the cell surface is a well documented structural feature, because their plasma membranes are potential target for chemotherapy. By using metabolic labeling ( [32P] phosphate, [3H]-myristic acid, [3H]-galactose) and by appropriate fractionated ...
M, Hublart +3 more
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Instability of the nuclear chromatin of procyclic Trypanosoma brucei brucei
Molecular and Biochemical Parasitology, 1989Digestion of nuclear chromatin of Trypanosoma brucei brucei procyclic culture forms with micrococcal nuclease yielded DNA fragments which formed DNA ladders in agarose gels, similar to those of rat liver. However, the chromatin of trypanosomes was digested more rapidly. The digestion of T. b.
Hecker H +3 more
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Effect of Trypanosoma brucei brucei on Erythropoiesis in Infected Rats
Journal of Parasitology, 2011Anemia generated from African trypanosome infection is considered an important symptom in humans and in domestic animals. In order to recover from anemia, the process of erythropoiesis is essential. Erythropoiesis is affected by erythropoietin (EPO), an erythropoietic hormone, supplying iron and inflammatory and proinflammatory cytokines.
Kazuhiko, Nishimura +5 more
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Characterization of the histones of Trypanosoma brucei brucei bloodstream forms
Acta Tropica, 1994Five groups of histones were shown in Trypanosoma brucei brucei, displaying qualitative and quantitative differences between two stages of the parasite's life-cycle. The influence of the histones of T. b. brucei bloodstream forms on the compaction pattern of the chromatin was investigated and their extractability in diluted acids and their amino acid ...
M, Burri +3 more
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Lipid composition of bloodstream forms of Trypanosoma brucei brucei
Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 1986The qualitative and quantitative lipid composition of bloodstream forms of Trypanosoma brucei brucei S42 was compared with that of rat plasma and erythrocytes. The concentrations of lipid-bound phosphate and lipid-bound sialic acid in the trypanosomes were markedly higher than those of the rodent tissues.
M, Carroll, P, McCrorie
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Trypanosoma brucei brucei: Polycations and the release of alanine aminotransferase
Experimental Parasitology, 1972Abstract Trypanosoma brucei brucei was exposed in vitro to the polycations protamine, poly- l -lysine and DEAE-dextran, and the effect was measured by the amount of l -alanine: 2-oxoglutarate aminotransferase (ALAT) (EC 2.6.15) released. Most was released after exposure to poly- l -lysine in a Tris buffer, less in a bicine buffer, and least in a
D G, Godfrey, A E, Taylor
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Characterization of different proteolytic activities in Trypanosoma brucei brucei
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 1992The variant surface glycoprotein of African trypanosomes is released after overnight incubation of parasites at 4 degrees C in pH 5.5 phosphate glucose buffer and may be purified by Concanavalin A Sepharose affinity chromatography. The addition of proteinase inhibitors during the parasite incubation is necessary to prevent the proteolysis of the ...
G, Huet +7 more
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Rodent-free cyclical transmission of Trypanosoma brucei brucei
Molecular and Biochemical Parasitology, 2017We provide a simple protocol enabling cyclical transmission of Trypanosoma brucei brucei to be performed without the need for mammals. These procedures have two advantages: they are in line with 3R principles of animal use - replace, refine, reduce - and may enable more laboratories to study the complete life cycle.
Arunasalam, Naguleswaran, Isabel, Roditi
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