Results 191 to 200 of about 32,780 (234)
Some of the next articles are maybe not open access.
The Ubiquitin-Ligase System in Trypanosoma Brucei Brucei
The American Journal of Tropical Medicine and Hygiene, 1993The question of whether the African trypanosome Trypanosoma brucei brucei utilizes a ubiquitin-ligase system to conjugate the 8,500-dalton protein ubiquitin to other proteins has not been investigated. Using 125I-labeled ubiquitin and gel electrophoresis (sodium dodecyl sulfate and acetic acid, urea, Triton X-100), we looked for the incorporation of ...
D J, Lowrie, B F, Giffin, R M, Ventullo
openaire +2 more sources
Characterisation of pentamidine-resistant Trypanosoma brucei brucei
Molecular and Biochemical Parasitology, 1995Following selection in vitro by exposure to increasing concentrations of the aromatic diamidine pentamidine, a Trypanosoma brucei brucei clone has been characterised in vivo and in vitro. The resistant clone, designated T.b. brucei S427/118/PR32.6, was found to be less virulent than the parental clone T.b.
Berger, Bradley J. +2 more
openaire +3 more sources
Effect of putrescine on the respiration of Trypanosoma brucei brucei
Molecular and Biochemical Parasitology, 1986When bloodstream forms of Trypanosoma brucei brucei were exposed to exogenous putrescine for 24 h during in vitro culture, the rate of O2 consumption increased significantly in a concentration-related and time-dependent manner. Trypanosomes cultured with 100 microM DL-alpha-difluoromethylornithine (DFMO), an irreversible inhibitor of ornithine ...
B F, Giffin, P P, McCann, C J, Bacchi
openaire +2 more sources
Experimental Parasitology, 1992
Macrophages collected from BCG-infected mice or exposed in vitro to interferon-gamma plus lipopolysaccharide developed a cytostatic activity on Trypanosoma brucei gambiense and Trypanosoma brucei brucei. This trypanostatic activity of activated macrophages was inhibited by addition of N-monomethyl-L-arginine, an inhibitor of the L-arginine-nitric oxide
P, Vincendeau +5 more
openaire +2 more sources
Macrophages collected from BCG-infected mice or exposed in vitro to interferon-gamma plus lipopolysaccharide developed a cytostatic activity on Trypanosoma brucei gambiense and Trypanosoma brucei brucei. This trypanostatic activity of activated macrophages was inhibited by addition of N-monomethyl-L-arginine, an inhibitor of the L-arginine-nitric oxide
P, Vincendeau +5 more
openaire +2 more sources
Characterization of different proteolytic activities in Trypanosoma brucei brucei
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 1992The variant surface glycoprotein of African trypanosomes is released after overnight incubation of parasites at 4 degrees C in pH 5.5 phosphate glucose buffer and may be purified by Concanavalin A Sepharose affinity chromatography. The addition of proteinase inhibitors during the parasite incubation is necessary to prevent the proteolysis of the ...
G, Huet +7 more
openaire +2 more sources
Effect of Trypanosoma brucei brucei on Erythropoiesis in Infected Rats
Journal of Parasitology, 2011Anemia generated from African trypanosome infection is considered an important symptom in humans and in domestic animals. In order to recover from anemia, the process of erythropoiesis is essential. Erythropoiesis is affected by erythropoietin (EPO), an erythropoietic hormone, supplying iron and inflammatory and proinflammatory cytokines.
Kazuhiko, Nishimura +5 more
openaire +2 more sources
Characterization of spermidine synthase from Trypanosoma brucei brucei
Molecular and Biochemical Parasitology, 1984Spermidine synthase from Trypanosoma brucei brucei was characterized and found to be similar to spermidine synthase from other sources. The Km for putrescine was found to be 0.2 mM and the Km for decarboxylated S-adenosylmethionine 0.1 microM. The approximate molecular weight of the enzyme was 74 000 as determined by a combination of molecular sieve ...
A J, Bitonti, S E, Kelly, P P, McCann
openaire +2 more sources
Presence of a lipophosphoglycan in two variants of trypanosoma brucei brucei
Biochemical and Biophysical Research Communications, 1988For the family of Trypanosomatidae (Trypanosoma and Leishmania) the organization of the glycoproteins on the cell surface is a well documented structural feature, because their plasma membranes are potential target for chemotherapy. By using metabolic labeling ( [32P] phosphate, [3H]-myristic acid, [3H]-galactose) and by appropriate fractionated ...
M, Hublart +3 more
openaire +2 more sources
Survival of Trypanosoma brucei brucei in cerebrospinal fluid
Annals of Tropical Medicine & Parasitology, 1992Different compositions of artificial cerebrospinal fluid (CSF) and the CSF from sleeping sickness patients both with and without late-stage [i.e. central nervous system (CNS)] involvement were evaluated for their abilities to support survival of Trypanosoma brucei brucei.
V W, Pentreath, A O, Owolabi, F, Doua
openaire +2 more sources
Instability of the nuclear chromatin of procyclic Trypanosoma brucei brucei
Molecular and Biochemical Parasitology, 1989Digestion of nuclear chromatin of Trypanosoma brucei brucei procyclic culture forms with micrococcal nuclease yielded DNA fragments which formed DNA ladders in agarose gels, similar to those of rat liver. However, the chromatin of trypanosomes was digested more rapidly. The digestion of T. b.
Hecker H +3 more
openaire +3 more sources