Results 31 to 40 of about 93 (89)
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BOVINE TRYPTASE: PURIFICATION AND CHARACTERIZATION
Biological Chemistry Hoppe-Seyler, 1992Bovine tryptase, a mast cell trypsin-like protease, was isolated from liver capsula and from mast cells obtained from the same tissue. The purification procedure which leads to an increase in tryptase activity of 850 fold, involves high salt extraction, hydrophobic interaction chromatography on octyl-Sepharose and affinity chromatography on heparin ...
Fiorucci, L, Erba, F, Ascoli, F
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Expression of α-tryptase and β-tryptase by human basophils
Journal of Allergy and Clinical Immunology, 2004Background α and β-Tryptase levels in serum are clinical tools for the evaluation of systemic anaphylaxis and systemic mastocytosis. Basophils and mast cells are known to produce these proteins. Objective The current study examines the effect of the α,β-tryptase genotype on basophil tryptase levels and the type of tryptase stored in these cells ...
Sherryline Jogie-Brahim +4 more
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Serum Concentrations of Tryptase in Children
Pediatric Allergy, Immunology, and Pulmonology, 2014Background: Mast cells are dispersed in many tissues, especially in digestive and respiratory-tract mucosal membranes. Tryptase, considered a marker of mast-cell activity, is the most important protease released from these cells during degranulation.
Katarzyna, Sznurkowska +7 more
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Total tryptase or β-tryptase in post mortem settings: Which is to be preferred?
Forensic Science International, 2018Dear Editor, We have read with attention and interest the Tsea et al. manuscript [1] entitled “Post mortem tryptase cut-off level for anaphylactic death” where a 5-year retrospective study has compared totaltryptase levels in the femoral blood of 9 anaphylactic deaths and 45 controls. A cut-off of 53.8 mg/L was identified for total post mortem tryptase
Zaami S., Busardo F. P.
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Expression of alpha-tryptase and beta-tryptase by human basophils.
The Journal of allergy and clinical immunology, 2004Alpha and beta-tryptase levels in serum are clinical tools for the evaluation of systemic anaphylaxis and systemic mastocytosis. Basophils and mast cells are known to produce these proteins.The current study examines the effect of the alpha,beta-tryptase genotype on basophil tryptase levels and the type of tryptase stored in these cells.Tryptase ...
Sherryline, Jogie-Brahim +4 more
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Archives of Biochemistry and Biophysics, 1991
Rat mast cell tryptase is located largely if not totally in the cell's secretory granules. When the active site reagent [3H]diisopropyl fluorophosphate was used to label tryptase and chymase simultaneously, the ratio of tryptase:chymase active sites was determined to be 0.05. In comparison to chymase and tryptase in other species and chymase in the rat,
D, Lagunoff, A, Rickard, C, Marquardt
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Rat mast cell tryptase is located largely if not totally in the cell's secretory granules. When the active site reagent [3H]diisopropyl fluorophosphate was used to label tryptase and chymase simultaneously, the ratio of tryptase:chymase active sites was determined to be 0.05. In comparison to chymase and tryptase in other species and chymase in the rat,
D, Lagunoff, A, Rickard, C, Marquardt
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Biomarkers of anaphylaxis, beyond tryptase
Current Opinion in Allergy & Clinical Immunology, 2015The purpose of this study is to describe the current knowledge regarding mediators involved in anaphylactic reactions, with a special focus on key effector cells and mechanisms.New insight into the potential relevance of pathways other than mast cell degranulation has been unravelled, such as the role of cytokines, platelet activation factor, lipid ...
Anna, Sala-Cunill, Victoria, Cardona
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