Results 91 to 100 of about 70,730 (308)

Multiplex strand displacement amplification (SDA) and detection of DNA sequences fromMycobacterium tuberculosisand other mycobacteria

Nucleic Acids Research, 1994
Strand Displacement Amplification (SDA) is an isothermal, in vitro method of amplifying a DNA target sequence prior to detection [Walker et al (1992) Nucleic Acids Res., 20, 1691-1693]. Here we describe a multiplex form of SDA that allows two target sequences and an internal amplification control to be co-amplified by a single pair of primers after ...
G T, Walker, J G, Nadeau, P A, Spears, J L, Schram, C M, Nycz, D D, Shank   +5 more
openaire   +3 more sources

Pseudo-outbreak of Mycobacterium gordonae in a teaching hospital: importance of strictly following decontamination procedures and emerging issues concerning sterilization [PDF]

, 2016
Aim of this study was to investigate a pseudo-outbreak of Mycobacterium gordonae analyzing isolates detected from clinical and environmental samples.
Alessia Cottarelli, Claudio M Mastroianni, Daniela M Cirillo, Fabio Mengoni, Lanfranco Fattorini, Laura Scorzolini, Lucia Marinelli, Maria De Giusti, MDPhD Laura Scorzolini, Rossella Baldan, Vincenzo Vullo   +10 more
core  

Consumed by Abdominal Distention

Arthritis Care &Research, EarlyView.
Abimbola Fadairo‐Azinge, James Grenert, Brian Haas, Mary Margaretten   +3 more
wiley   +1 more source

Mass‐Produced and High‐Performance Nanowell Biosensor Fabricated via Semiconductor Manufacturing for Rapid and Accurate COVID‐19 Diagnosis in the Clinical Field

Advanced Science, EarlyView.
A semiconductor‐fabricated nanowell biosensor enables rapid, scalable, and highly reproducible detection of SARS‐CoV‐2 antigens from nasal swabs within ∼10 minutes. Clinical validation in 249 retrospective and 243 prospective patient samples demonstrates high sensitivity and specificity, minimal cross‐reactivity, and robust batch‐to‐batch ...
Yoo Min Park, Zahra Rezaei, Nam Ho Bae, Donggee Rho, Da‐Seul Kim, EunYoung Go, YoungTae Seo, Seok Jae Lee, Won Chan Seo, Luke P. Lee, HeaYeon Lee, Su Ryon Shin   +11 more
wiley   +1 more source

Simple Estimates for Local Prevalence of Latent Tuberculosis Infection, United States, 2011–2015

Emerging Infectious Diseases, 2018
We used tuberculosis genotyping results to derive estimates of prevalence of latent tuberculosis infection in the United States. We estimated
Maryam B. Haddad, Kala M. Raz, Timothy L. Lash, Andrew N. Hill, J. Steve Kammerer, Carla A. Winston, Kenneth G. Castro, Neel R. Gandhi, Thomas R. Navin   +8 more
doaj   +1 more source

From Cell‐Free Transcriptomes to Single‐Cell Landscapes: Biomarker Discovery and Originating Cell Alteration Analysis via Graph Matrix Factorization

Advanced Science, EarlyView.
CellFreeGMF traces plasma cfRNA to likely originating cell types by integrating single‐cell atlases with graph‐regularized matrix factorization. The method decomposes cfRNA profiles into sample–cell contributions to reconstruct pseudo single‐cell expression.
Wenxiang Zhang, Wenjing Zhang, Hang Wei, Shiyan Liu, Junliang Shang, Weijie Gong, Hanwen Cheng, Xiujuan Lei, Yuhui Kou, Baoguo Jiang   +9 more
wiley   +1 more source

Risk of acquired drug resistance during short-course directly observed treatment of tuberculosis in an area with high levels of drug resistance. [PDF]

, 2007
BACKGROUND: Data on the performance of standardized short-course directly observed treatment (DOTS) of tuberculosis (TB) in areas with high levels of drug resistance and on the potential impact of DOTS on amplification of resistance are limited ...
Cox, Cox, Cox, Crofton, D. Doshetov, Das, Dewan, Drobniewski, Espinal, Farmer, G. Ismailov, Glynn, H. S. Cox, Heifets, J. D. Orozco, Kamerbeek, Kubica, L. Blok, Laszlo, Madison, McNabb, Mitchison, Niemann, Pardini, Quy, Reeler, S. Niemann, S. R. Gerdes, Shamputa, van Embden, van Rie, Wilkinson, Y. Kebede, Yoshiyama   +33 more
core   +2 more sources

Metabolism‐Based Biomarkers for Rapid Phenotypic Antibiotic Susceptibility Testing

Advanced Science, EarlyView.
Metabolic differences emerging after antibiotic exposure provide rapid phenotypic signals of susceptibility and resistance. This review summarizes metabolic biomarkers for rapid phenotypic AST, associated with nutrient uptake, respiratory activity, metabolic reprogramming, and enzymatic function. ABSTRACT The accelerating global crisis of antimicrobial
Sha Yu, Bayinqiaoge, Xi Lu, Xin Wang, Rongfeng Wang, Yi Li, Shi‐Yang Tang, Chengchen Zhang   +7 more
wiley   +1 more source

Association of Diabetes and Tuberculosis Disease among US-Bound Adult Refugees, 2009–2014

Emerging Infectious Diseases, 2017
Diabetes is associated with an increased risk for active tuberculosis (TB) disease. We conducted a case–control study and found a significant association between diabetes and TB disease among US-bound refugees.
Stephen R. Benoit, Edward W. Gregg, Sasi Jonnalagadda, Christina R. Phares, Weigong Zhou, John A. Painter   +5 more
doaj   +1 more source

Accelerating Primary Screening of USP8 Inhibitors from Drug Repurposing Databases with Tree‐Based Machine Learning

Advanced Intelligent Discovery, EarlyView.
This study introduces a tree‐based machine learning approach to accelerate USP8 inhibitor discovery. The best‐performing model identified 100 high‐confidence repurposable compounds, half already approved or in clinical trials, and uncovered novel scaffolds not previously studied. These findings offer a solid foundation for rapid experimental follow‐up,
Yik Kwong Ng, Sharon Ai Er Lam, Wei Chun Lim, Hannah Hui Min Toh, Shi Jun Ang   +4 more
wiley   +1 more source