Results 1 to 10 of about 4,954 (99)

The Game of Tubulins

Cells, 2021
Members of the tubulin superfamily are GTPases; the activities of GTPases are necessary for life. The members of the tubulin superfamily are the constituents of the microtubules and the γ-tubulin meshwork.
Maria Alvarado Kristensson
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Reconstituting Microtubules: A Decades-Long Effort From Building Block Identification to the Generation of Recombinant α/β-Tubulin

Frontiers in Cell and Developmental Biology, 2022
Microtubules are cytoskeletal filaments underlying the morphology and functions of all eukaryotic cells. In higher eukaryotes, the basic building blocks of these non-covalent polymers, ɑ- and β-tubulins, are encoded by expanded tubulin family genes (i.e.,
Shih-Chieh Ti
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SRC Kinase-Mediated Tyrosine Phosphorylation of TUBB3 Regulates Its Stability and Mitotic Spindle Dynamics in Prostate Cancer Cells

Pharmaceutics, 2022
Tubulin is an integral part of the cytoskeleton and plays a pivotal role in cellular signaling, maintenance, and division. β-tubulin is also the molecular target for taxane compounds such as docetaxel (DTX) and cabazitaxel (CTX), both first-line ...
Alan Alfano, Jin Xu, Xi Yang, Dhanraj Deshmukh, Yun Qiu   +4 more
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Design, Synthesis and Cytotoxic Activity Evaluation of Newly Synthesized Amides-Based TMP Moiety as Potential Anticancer Agents over HepG2 Cells

Molecules, 2022
A novel series of amides based TMP moiety was designed, synthesized and evaluated for their antiproliferative as well as enzyme inhibition activity. Compounds 6a and 6b showed remarkable cytotoxic activity against HepG2 cells with IC50 values 0.65 and 0 ...
Tarfah Al-Warhi, Adil Aldhahrani, Fayez Althobaiti, Eman Fayad, Ola A. Abu Ali, Sarah Albogami, Ali H. Abu Almaaty, Amgad I. M. Khedr, Syed Nasir Abbas Bukhari, Islam Zaki   +9 more
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Reformulation of an extant ATPase active site to mimic ancestral GTPase activity reveals a nucleotide base requirement for function

eLife, 2021
Hydrolysis of nucleoside triphosphates releases similar amounts of energy. However, ATP hydrolysis is typically used for energy-intensive reactions, whereas GTP hydrolysis typically functions as a switch.
Taylor B Updegrove, Jailynn Harke, Vivek Anantharaman, Jin Yang, Nikhil Gopalan, Di Wu, Grzegorz Piszczek, David M Stevenson, Daniel Amador-Noguez, Jue D Wang, L Aravind, Kumaran S Ramamurthi   +11 more
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Actin and Microtubules Differently Contribute to Vacuolar Targeting Specificity during the Export from the ER

Membranes, 2021
Plants rely on both actin and microtubule cytoskeletons to fine-tune sorting and spatial targeting of membranes during cell growth and stress adaptation.
Monica De Caroli, Fabrizio Barozzi, Luciana Renna, Gabriella Piro, Gian-Pietro Di Sansebastiano   +4 more
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Organization of the Cytoskeleton in Ectopic Foci of the Endometrium with Rare Localization

Biomedicines, 2021
(1) Background: Endometriosis is a common pathology of the female reproductive system, often accompanied by pain and decreased fertility. However, its pathogenesis has not been sufficiently studied regarding the role of the cytoskeleton.
Konstantin A. Toniyan, Victoria V. Povorova, Elena Yu. Gorbacheva, Valery V. Boyarintsev, Irina V. Ogneva   +4 more
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Housekeeping Proteins in Meat Quality Research: Are They Reliable Markers for Internal Controls in Western Blot? A Mini Review

Meat and Muscle Biology, 2022
Advancements in technology and analytical methods enable researchers to explore the biochemical events that cause variation in meat quality. Among those, western blot techniques have been successfully used in identifying and quantifying the key proteins ...
Chaoyu Zhai, Elisabeth J. Huff-Lonergan, Mahesh N. Nair, Steven M. Lonergan   +3 more
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Evolutionary Divergence of Enzymatic Mechanisms for Tubulin Detyrosination

Cell Reports, 2019
Summary: The two related members of the vasohibin family, VASH1 and VASH2, encode human tubulin detyrosinases. Here we demonstrate that, in contrast to VASH1, which requires binding of small vasohibin binding protein (SVBP), VASH2 has autonomous tubulin ...
Siem van der Laan, Maude F. Lévêque, Guillaume Marcellin, Lubomir Vezenkov, Yoann Lannay, Geronimo Dubra, Guillaume Bompard, Sara Ovejero, Serge Urbach, Andrew Burgess, Muriel Amblard, Yvon Sterkers, Patrick Bastien, Krzysztof Rogowski   +13 more
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Tubulin isotype regulation maintains asymmetric requirement for α-tubulin over β-tubulin

Journal of Cell Biology, 2023
How cells regulate α- and β-tubulin to meet the demand for αβ-heterodimers and avoid consequences of monomer imbalance is not understood. We investigate the role of gene copy number and how shifting expression of α- or β-tubulin genes impacts tubulin proteostasis and microtubule function in Saccharomyces cerevisiae.
Linnea C. Wethekam, Jeffrey K. Moore
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