Results 171 to 180 of about 8,951 (194)

Effects of tyrosine-26 and tyrosine-64 nitration on the photoreactions of bacteriorhodopsin

Biochemistry, 1985
In the dark, all titratable tyrosine residues of bacteriorhodopsin have pK's of greater than 11.0, which may be caused by the hydrophobic environment for buried residues and by high negative charge density for surface residues [Scherrer, P., & Stoeckenius, W. (1984) Biochemistry 23, 6195-6202].
P, Scherrer, W, Stoeckenius
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Mass Mapping Sites of Nitration in Tyrosine Hydroxylase:  Random vs Selective Nitration of Three Tyrosine Residues

Chemical Research in Toxicology, 2003
Previous work has shown that tyrosine residues Y423, Y428, and Y432 are potential targets for nitration when tyrosine hydroxylase is exposed to peroxynitrite. For any given protein molecule, up to three nitration events involving these residues may occur.
Chad R, Borges, Donald M, Kuhn, J Throck, Watson   +2 more
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Modifications of tyrosine and catecholamines by peroxynitrite nitrite and nitrate

Frontiers in Bioscience, 2003
At pH 7.0 in non-"deaired" potassium phosphate buffer, the reactions of L-tyrosine, L-dopa, dopamine, L-norepinephrine, and L-epinephrine with peroxynitrite (PN) or nitrite, generated colored products. These products displayed not only unique colors and patterns of mobility on silica thin layer chromatographic plates, but also varied increase of ...
Wu-Nan, Kuo, Joseph M, Kocis, Luvina K, Guruvadoo   +2 more
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Neopterin Derivatives Modulate the Nitration of Tyrosine by Peroxynitrite

Biochemical and Biophysical Research Communications, 1998
The impact of neopterin and 7,8-dihydroneopterin on peroxynitrite-induced nitration of l-tyrosine was studied. Neopterin derivatives and peroxynitrite are formed during immune response. Tyrosine nitration represents one major effect of nitric oxide-mediated cytotoxicity.
B, Widner, G, Baier-Bitterlich, I, Wede, B, Wirleitner, D, Fuchs   +4 more
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Proteomic Method for Identification of Tyrosine-Nitrated Proteins

2004
Biologic nitration of protein tyrosine (to form 3-nitrotyrosine) is a recently described phenomenon that is associated with many diseases. We have devised a proteomic methodology to identify these modified proteins. This utilizes protein fractionation by two-dimensional polyacrylamide gel electrophoresis (2-D PAGE), partial transfer onto polyvinylidene
Kulwant S, Aulak, Thomas, Koeck, John W, Crabb, Dennis J, Stuehr   +3 more
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Nitration of tyrosine and its effect on DNA hybridization

Biosensors and Bioelectronics, 2018
One major marker of nitrosative stress is the formation of 3-Nitrotyrosine (3-NT) from Tyrosine (Tyr) by adding a nitro group (-NO2) with nitrating agents. Nitration of Tyr often causes loss of protein activity and is linked with many diseases. In this article, we detect 3-NT and discriminate it from Tyr with Differential Pulse Voltammetry (DPV) as it ...
Topkaya, Seda Nur, Ozyurt, Vasfiye Hazal, Cetin, Arif E., Otles, Semih   +3 more
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Identification of tyrosine nitration in UCH‐L1 and GAPDH

ELECTROPHORESIS, 2011
AbstractProtein tyrosine nitration is a post‐translational modification commonly used as a marker of cellular oxidative stress associated with numerous pathophysiological conditions. We focused on ubiquitin carboxyl terminal hydrolase‐L1 (UCH‐L1) and glyceraldehyde‐3‐phosphate (GAPDH) which are high‐abundant brain proteins that have been identified to ...
Joy D, Guingab-Cagmat, Stanley M, Stevens, Mary V, Ratliff, Zhiqun, Zhang, Mark S, Gold, John, Anagli, Kevin K W, Wang, Firas H, Kobeissy   +7 more
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Protein Tyrosine Nitration in Hyperammonemia and Hepatic Encephalopathy

Metabolic Brain Disease, 2005
Hepatic encephalopathy is seen as a clinical manifestation of a chronic low grade cerebral edema, which is thought to trigger disturbances of astrocyte function, glioneuronal communication, and finally HE symptoms. In cultured astrocytes, hypoosmotic swelling triggers a rapid oxidative stress response, which involves the action of NADPH oxidase ...
Dieter, Häussinger, Boris, Görg, Roland, Reinehr, Freimut, Schliess   +3 more
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Peroxynitrite and protein tyrosine nitration of prostacyclin synthase

Prostaglandins & Other Lipid Mediators, 2007
When working on the regulation of prostacyclin synthase (PGIS), we found that PGIS was selectively inhibited by peroxynitrite (ONOO-), a potent oxidant formed by the combination of superoxide anion and nitric oxide (NO) at a rate of diffusion-controlled. None of the cellular antioxidants studied (i.e.
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Protein tyrosine nitration and peroxynitrite

The FASEB Journal, 2002
William M. Deen, Steven R. Tannenbaum, Joseph S. Beckman   +2 more
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