Results 21 to 30 of about 205,532 (202)
A genetic assay for transcription errors reveals multilayer control of RNA polymerase II fidelity. [PDF]
PLoS Genetics, 2014 We developed a highly sensitive assay to detect transcription errors in vivo. The assay is based on suppression of a missense mutation in the active site tyrosine in the Cre recombinase.
Jordan D Irvin +6 more
doaj +1 more source
The Site-Specific Recombination System of the Escherichia coli Bacteriophage Φ24B
Frontiers in Microbiology, 2020 Stx bacteriophages are members of the lambdoid group of phages and are responsible for Shiga toxin (Stx) production and the dissemination of Shiga toxin genes (stx) across shigatoxigenic Escherichia coli (STEC).
Mohammed Radhi Mohaisen +4 more
doaj +1 more source
The XerC recombinase ofProteus mirabilis: characterization and interaction with other tyrosine recombinases [PDF]
FEMS Microbiology Letters, 2003 XerC and XerD are two site-specific recombinases, which act on different sites to maintain replicons in a monomeric state. This system, which was first discovered and studied in Escherichia coli, is present in several species including Proteus mirabilis, where the XerD recombinase was previously characterized by our laboratory. In this paper, we report
Manuela, Villion, George, Szatmari
openaire +2 more sources
Dual recombinase action in the normal and neoplastic mammary gland epithelium
Scientific Reports, 2021 We developed a transgenic mouse line that expresses the codon-optimized Flp recombinase under the control of the MMTV promoter in luminal epithelial cells of the mammary gland.
Patrick D. Rädler +9 more
doaj +1 more source
Staphylococcal Phages Adapt to New Hosts by Extensive Attachment Site Variability
mBio, 2021 Bacterial pathogens commonly carry prophages that express virulence factors, and human strains of Staphylococcus aureus carry Sa3int phages, which promote immune evasion.
Helena Leinweber +4 more
doaj +1 more source
Determinants of selectivity in Xer site-specific recombination [PDF]
, 1996 A remarkable property of some DNA-binding proteins that can interact with and pair distant DNA segments is that they mediate their biological function only when their binding sites are arranged in a specific configuration. Xer site-specific recombination
Blakely, G, Sherratt, D
core +1 more source
An att site-based recombination reporter system for genome engineering and synthetic DNA assembly
BMC Biotechnology, 2017 Background Direct manipulation of the genome is a widespread technique for genetic studies and synthetic biology applications. The tyrosine and serine site-specific recombination systems of bacteriophages HK022 and ΦC31 are widely used for stable ...
Michael J. Bland +3 more
doaj +1 more source
Scientific Reports, 2021 Due to redundancy with other tyrosine phosphatases, the ubiquitously expressed tyrosine phosphatase SHP-2 (encoded by Ptpn11) is not required for T cell development. However, Ptpn11 gene deletion driven by CD4 Cre recombinase leads to cartilage tumors in
Jeffrey T. McNamara +4 more
doaj +1 more source
The catalytic residues of Tn3 resolvase [PDF]
, 2009 To characterize the residues that participate in the catalysis of DNA cleavage and rejoining by the site-specific recombinase Tn3 resolvase, we mutated conserved polar or charged residues in the catalytic domain of an activated resolvase variant.
Olorunniji, F.J. +5 more
core +1 more source