Results 31 to 40 of about 59,465 (263)
Isoforms of mammalian ubiquitin-activating enzyme.
Journal of Biological Chemistry, 1992 Ubiquitin-activating enzyme, "E1," is the first enzyme in the pathway leading to formation of ubiquitin-protein conjugates and represents a potential target for regulation in the metabolic control of the conjugation reaction. Antiserum raised against human E1 recognizes two immunoreactive proteins in extracts from several human cell lines and animal ...
J C, Cook, P B, Chock
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Pleiotropic Effects of ATP·Mg2+ Binding in the Catalytic Cycle of Ubiquitin-activating Enzyme [PDF]
Journal of Biological Chemistry, 2006 Conjugation of ubiquitin and other Class 1 ubiquitin-like polypeptides to specific protein targets serves diverse regulatory functions in eukaryotes. The obligatory first step of conjugation requires ATP-coupled activation of the ubiquitin-like protein by members of a superfamily of evolutionarily related enzymes. Kinetic and equilibrium studies of the
Zeynep, Tokgöz +2 more
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VEXAS Syndrome: A Call for Diagnostic Awareness Based on a Case Series of Seven Patients
Acta Médica Portuguesa, 2023 N/a.
Filipe R. Pinto +4 more
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In vitro Ubiquitin Dimer Formation Assay
Bio-Protocol, 2017 The process of protein ubiquitination typically consists of three sequential steps to add an ubiquitin (Ub) or Ub chain to a substrate protein, requiring three different enzymes, ubiquitin activating enzyme (E1), ubiquitin conjugating enzyme (E2), and ...
Sheng Wang, Ling Cao, Hong Wang
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"Covalent affinity" purification of ubiquitin-activating enzyme.
Journal of Biological Chemistry, 1982 We have previously described an enzyme that activates ubiquitin, the heat-stable polypeptide of the ATP-dependent proteolytic system from reticulocytes (Ciechanover, A., Heller, H., Katz-Etzion, R., and Hershko, A. (1981) Proc. Natl. Acad. Sci. U. S. A. 78, 761-765). It carries out ubiquitin-dependent PPi-ATP and AMP-ATP exchange reactions and binds to
A, Ciechanover +3 more
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Structural Insights into E1-Catalyzed Ubiquitin Activation and Transfer to Conjugating Enzymes [PDF]
Cell, 2008 Ubiquitin (Ub) and ubiquitin-like proteins (Ubls) are conjugated to their targets by specific cascades involving three classes of enzymes, E1, E2, and E3. Each E1 adenylates the C terminus of its cognate Ubl, forms a E1 approximately Ubl thioester intermediate, and ultimately generates a thioester-linked E2 approximately Ubl product. We have determined
Lee, Imsang, Schindelin, Hermann
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HyperISGylation of Old World monkey ISG15 in human cells. [PDF]
PLoS ONE, 2008 BACKGROUND: ISG15 is an Ubiquitin-like protein, highly induced by Type I Interferons. Upon the cooperative activity of specific Ubiquitinating enzymes, ISG15 can be conjugated to its substrates. Increasing evidence points to a role for protein ISGylation
Els Pattyn +8 more
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Frontiers in Endocrinology, 2023 Ubiquitin-fold modifier 1 (UFM1) is a ubiquitin-like molecule (UBL) discovered almost two decades ago, but our knowledge about the cellular and molecular mechanisms of this novel protein post-translational modification is still very fragmentary.
Yun Cheng +3 more
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Mathematical model for the ubiquitin activating enzyme E1
Journal of Biomedical Science and Engineering, 2010 The ubiquitin-activating enzyme E1 (EC 6.3.2.19) represents the first step in the degradation of proteins by the ubiquitin proteasome pathway. E1 transfers ubiquitin from the ubiquitinated E1 to the ubiquitin carrier proteins (E2), ubiquitin-protein ligases (E3) and proteins. This process is rather complex, and known from the work of Haas, Ciechanover,
Francisco Javier López-Cánovas +3 more
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Phosphorylation of ubiquitin-activating enzyme in cultured cells.
Proceedings of the National Academy of Sciences, 1995 Ubiquitin-activating enzyme, E1, is the first enzyme in the pathway leading to formation of ubiquitin-protein conjugates. E1 exists as two isoforms in human cells which are separable by electrophoresis. These isoforms migrate with apparent molecular sizes of 110 kDa and 117 kDa in SDS/polyacrylamide gels.
J C, Cook, P B, Chock
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