Results 21 to 30 of about 59,465 (263)
Synthesis of (di)nucleoside polyphosphates by the ubiquitin activating enzyme E1 [PDF]
FEBS Letters, 2005 Previous work from this laboratory had shown that ligases may catalyze the synthesis of (di)nucleoside polyphosphates. Here, we show that one of the enzymes of the proteasome system (E1 or the ubiquitin (Ub) activating enzyme, EC 6.3.2.19) catalyzes very effectively (k cat = 0.29 ± 0.05 s−1) the transfer of AMP from the E–AMP–ubiquitin complex to ...
Günther Sillero, Maria A. +3 more
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E3 ubiquitin ligases: styles, structures and functions
Molecular Biomedicine, 2021 E3 ubiquitin ligases are a large family of enzymes that join in a three-enzyme ubiquitination cascade together with ubiquitin activating enzyme E1 and ubiquitin conjugating enzyme E2.
Quan Yang +3 more
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Journal of Fungi, 2021 Csn5 is a subunit ofthe COP9/signalosome complex in model fungi. Here, we report heavier accumulation of orthologous Csn5 in the nucleus than in the cytoplasm and its indispensability to insect pathogenicity and virulence-related cellular events of ...
Ya-Ni Mou +4 more
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An Essential Yeast Gene Encoding a Homolog of Ubiquitin-activating Enzyme [PDF]
Journal of Biological Chemistry, 1995 Ubiquitin (Ub) activation by the Ub-activating (E1) enzyme is the initial and essential step common to all of the known processes that involve post-translational conjugation of Ub to itself or other proteins. The "activated" Ub, linked via a thioester bond to a specific cysteine residue in one of several Ub-conjugating (E2) enzymes, which catalyze the ...
Dohmen, R. Jürgen +6 more
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Proteasome inhibitors: new class of antitumor agents
Biomolecules & Biomedicine, 2003 The ubiquitin-proteasome pathway is the principal pathway for intracellular protein degradation1,2 (Fig 1). This pathway selectively degrades an extensive number of short-lived regulatory proteins involved in the control of normal cellular processes. In
Gordan Srkalović
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Nature Communications, 2023 UBA1 is the primary E1 ubiquitin-activating enzyme responsible for generation of activated ubiquitin required for ubiquitination, a process that regulates stability and function of numerous proteins.
Wenjing Yan +17 more
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In vitro Di-ubiquitin Formation Assay and E3 Cooperation Assay
Bio-Protocol, 2022 Ubiquitination is a post-translational modification conserved across eukaryotic species. It contributes to a variety of regulatory pathways, including proteasomal degradation, DNA repair, and cellular differentiation.
Rebecca Burge +3 more
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UBA 1: an essential yeast gene encoding ubiquitin‐activating enzyme. [PDF]
The EMBO Journal, 1991 All known functions of ubiquitin are mediated through its covalent attachment to other proteins. The post-translational formation of ubiquitin--protein conjugates is preceded by an ATP-requiring step in which the carboxyl terminus of ubiquitin is adenylated and subsequently joined, through a thiolester bond, to a cysteine residue in the ubiquitin ...
McGrath, John P. +2 more
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Incorporating distant sequence features and radial basis function networks to identify ubiquitin conjugation sites. [PDF]
PLoS ONE, 2011 Ubiquitin (Ub) is a small protein that consists of 76 amino acids about 8.5 kDa. In ubiquitin conjugation, the ubiquitin is majorly conjugated on the lysine residue of protein by Ub-ligating (E3) enzymes.
Tzong-Yi Lee +3 more
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Life Science Alliance, 2021 This study shows that ubiquitin conjugates associate with various types of stress granules and that an active ubiquitin system is required for the efficient clearance of some types of stress granules.
Nazife Tolay, Alexander Buchberger
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