Results 31 to 40 of about 519,123 (352)

Site-specific identification and quantitation of endogenous SUMO modifications under native conditions. [PDF]

, 2017
Small ubiquitin-like modifier (SUMO) modification regulates numerous cellular processes. Unlike ubiquitin, detection of endogenous SUMOylated proteins is limited by the lack of naturally occurring protease sites in the C-terminal tail of SUMO proteins ...
Ahmad, Alla S, Bennett, Eric J, Clauser, Karl R, Gu, Hongbo, Komives, Elizabeth A, Leonard, Marilyn, Lumpkin, Ryan J, Meyer, Jesse G, Zhu, Yiying   +8 more
core   +2 more sources

Interplay between the Ubiquitin Proteasome System and Ubiquitin-Mediated Autophagy in Plants

Cells, 2020
All eukaryotes rely on the ubiquitin-proteasome system (UPS) and autophagy to control the abundance of key regulatory proteins and maintain a healthy intracellular environment. In the UPS, damaged or superfluous proteins are ubiquitinated and degraded in
Tong Su, Mingyue Yang, Pingping Wang, Yanxiu Zhao, Changle Ma   +4 more
doaj   +1 more source

RBR E3 ubiquitin ligases: new structures, new insights, new questions [PDF]

, 2014
The RBR (RING-BetweenRING-RING) or TRIAD [two RING fingers and a DRIL (double RING finger linked)] E3 ubiquitin ligases comprise a group of 12 complex multidomain enzymes.
Shaw, Gary S., Spratt, Donald E., Walden, Helen   +2 more
core   +1 more source

Ubiquitination of GPCRs [PDF]

, 2011
In this chapter, we describe a method for detecting the ubiquitination status of G protein-coupled receptors (GPCRs). This involves co-expression of a GPCR with an epitope-tagged ubiquitin construct in a -heterologous mammalian expression system. Stimulus-dependent modification of the GPCR by -ubiquitin is detected by immunoprecipitation and subsequent
Adriana, Caballero, Adriano, Marchese
openaire   +2 more sources

Ubiquitin in Motion: Structural Studies of the Ubiquitin-Conjugating Enzyme∼Ubiquitin Conjugate [PDF]

Biochemistry, 2011
Ubiquitination of proteins provides a powerful and versatile post-translational signal in the eukaryotic cell. The formation of a thioester bond between ubiquitin (Ub) and the active site of a ubiquitin-conjugating enzyme (E2) is critical for the transfer of Ub to substrates. Assembly of a functional ubiquitin ligase (E3) complex poised for Ub transfer
Jonathan N, Pruneda, Kate E, Stoll, Laura J, Bolton, Peter S, Brzovic, Rachel E, Klevit   +4 more
openaire   +2 more sources

Cryopreservation of fluorescence activated cell sorted boar spermatozoa based on extracellular ubiquitination

Czech Journal of Animal Science, 2016
The present study is focused on the methodology of fluorescence activated cell sorting (FACS) of spermatozoa stained by the antibody against extracellular surface marker ubiquitin (eUb) and subsequent protocol for their long term storage in liquid ...
A. Petelák, V. Krylov
doaj   +1 more source

Immunogenic cell death triggered by impaired deubiquitination in multiple myeloma relies on dysregulated type I interferon signaling

Frontiers in Immunology, 2023
IntroductionProteasome inhibition is first line therapy in multiple myeloma (MM). The immunological potential of cell death triggered by defects of the ubiquitin-proteasome system (UPS) and subsequent perturbations of protein homeostasis is, however ...
Zeinab Waad Sadiq, Annamaria Brioli, Annamaria Brioli, Ruba Al-Abdulla, Gonca Çetin, Jacqueline Schütt, Hugo Murua Escobar, Elke Krüger, Frédéric Ebstein   +8 more
doaj   +1 more source

Triad3a induces the degradation of early necrosome to limit RipK1-dependent cytokine production and necroptosis. [PDF]

, 2018
Understanding the molecular signaling in programmed cell death is vital to a practical understanding of inflammation and immune cell function. Here we identify a previously unrecognized mechanism that functions to downregulate the necrosome, a central ...
A Degterev, A Degterev, A Kaczmarek, A Polykratis, B Shutinoski, C Fearns, CP Dillon, D Ofengeim, DE Christofferson, DM Monack, DM Moquin, DR Green, EW Harhaj, F Ginhoux, G Dougan, H Sakamoto, I Jaco, J Hitomi, JW Upton, K Newton, KE Lawlor, L Duprez, L Eckmann, L Galluzzi, LA O’Neill, M Belosevic, M Pasparakis, MA Brennan, MJ Bertrand, N Arpaia, N Bidere, N Festjens, N Robinson, N Shembade, N Takahashi, N Vanlangenakker, N. Peltzer, P Mandal, P Vandenabeele, P Vandenabeele, R Bellamy, R Patel, RE Voll, S Akira, S He, S He, S Malladi, S McComb, S McComb, S McComb, S McComb, SB Berger, SC Sun, SP Salcedo, ST Beug, T McQuade, T Vanden Berghe, T Xie, TH Chuang, W Declercq, W Reiley, WD Cook, WJ Kaiser, WW Reiley, XN Wu, Y Dondelinger, YS Cho, Z Cai   +67 more
core   +2 more sources

SUMO conjugation regulates immune signalling

Fly, 2020
Post-translational modifications (PTMs) are critical drivers and attenuators for proteins that regulate immune signalling cascades in host defence. In this review, we explore functional roles for one such PTM, the small ubiquitin-like modifier (SUMO ...
Sushmitha Hegde, Amarendranath Soory, Bhagyashree Kaduskar, Girish S. Ratnaparkhi   +3 more
doaj   +1 more source

Processing of the ribosomal ubiquitin-like fusion protein FUBI-eS30/FAU is required for 40S maturation and depends on USP36

eLife, 2021
In humans and other holozoan organisms, the ribosomal protein eS30 is synthesized as a fusion protein with the ubiquitin-like protein FUBI. However, FUBI is not part of the mature 40S ribosomal subunit and cleaved off by an as-of-yet unidentified ...
Jasmin van den Heuvel, Caroline Ashiono, Ludovic C Gillet, Kerstin Dörner, Emanuel Wyler, Ivo Zemp, Ulrike Kutay   +6 more
doaj   +1 more source