Integration of metabolomics and proteomics reveals the mechanisms of seasonal accumulation and key regulatory networks of polysaccharides in Polygonatum odoratum. [PDF]
Zhu Y, Liang S, Fu D, Fu H, Zhao H.
europepmc +1 more source
IMACulate(DE3), an E. coli Strain for High Purity His‐Tagged Protein Purifications
ABSTRACT Immobilised Metal Affinity Chromatography (IMAC) is widely used to purify his‐tagged recombinant proteins from Escherichia coli. However, endogenous contaminants with histidine clusters, such as GFAT and PDH E1 proteins, are often co‐purified with the target protein.
Jia Q. Truong +5 more
wiley +1 more source
A Modular Nucleoside Kinase Cascade for the Synthesis of Ribonucleoside Triphosphates
Damm et al. report standardized enzymatic cascades for production of all canonical nucleoside triphosphates from the corresponding nucleosides, using kinase‐catalyzed phosphorylation from acetyl phosphate. Reactions afforded ∼ 100 mM product in near‐quantitative conversion.
Oliver T. Damm +3 more
wiley +1 more source
A lightweight machine learning approach for DDoS detection and classification. [PDF]
Ebrahem O, Dowaji S, Alhammoud S.
europepmc +1 more source
PharmVar GeneFocus: CYP1A2—Clinical Impact, Genetic Variation, and Updated Nomenclature
The Pharmacogene Variation Consortium (PharmVar) provides nomenclature for the highly polymorphic human CYP1A2 gene. CYP1A2 plays a crucial role in the biotransformation of several commonly used drugs, including antipsychotics, antidepressants, anxiolytics, and methylxanthines.
Katalin Monostory +13 more
wiley +1 more source
Molecular basis of collagen galactosylation by GLT25D1. [PDF]
Sun H +15 more
europepmc +1 more source
Benchmarking Non‐Port‐Hashing Routers With Multiple IP Addresses: Test Setup Recommendations
Contemporary routers distribute the load among their processing elements by using IP addresses and/or port numbers for hashing. Unbiased benchmarking of non‐port‐hashing routers requires the use of pseudorandom IP addresses. Two test setups are examined: a direct connection between the Tester and the Device Under Test, and a gateway‐based approach ...
Gábor Lencse, Keiichi Shima, Ole Trøan
wiley +1 more source
Structure-coevolution dual-guided engineering of C-glycosyltransferase enables high-level biosynthesis of vitexin in <i>Yarrowia lipolytica</i>. [PDF]
Shi CQ +7 more
europepmc +1 more source

