Results 1 to 10 of about 44,652 (214)

High level in vivo mucin-type glycosylation in Escherichia coli

Microbial Cell Factories, 2018
Background Increasing efforts have been made to assess the potential of Escherichia coli strains for the production of complex recombinant proteins. Since a considerable part of therapeutic proteins are glycoproteins, the lack of the post-translational ...
Phillipp Mueller, Rahul Gauttam, Nadja Raab, René Handrick, Claudia Wahl, Sebastian Leptihn, Michael Zorn, Michaela Kussmaul, Marianne Scheffold, Bernhard Eikmanns, Lothar Elling, Sabine Gaisser   +11 more
doaj   +1 more source

Hydrophobic recognition allows the glycosyltransferase UGT76G1 to catalyze its substrate in two orientations

Nature Communications, 2019
UGT76G1 is an UDP-glucose dependent glycosyltransferase and a component of the biosynthesis pathway for the natural sugar substitute steviol glycoside.
Ting Yang, Jinzhu Zhang, Dan Ke, Wenxian Yang, Minghai Tang, Jian Jiang, Guo Cheng, Jianshu Li, Wei Cheng, Yuquan Wei, Qintong Li, James H. Naismith, Xiaofeng Zhu   +12 more
doaj   +1 more source

Rare ginsenoside Ia synthesized from F1 by cloning and overexpression of the UDP-glycosyltransferase gene from Bacillus subtilis: synthesis, characterization, and in vitro melanogenesis inhibition activity in BL6B16 cells

Journal of Ginseng Research, 2018
Background: Ginsenoside F1 has been described to possess skin-whitening effects on humans. We aimed to synthesize a new ginsenoside derivative from F1 and investigate its cytotoxicity and melanogenesis inhibitory activity in B16BL6 cells using ...
Dan-Dan Wang, Yan Jin, Chao Wang, Yeon-Ju Kim, Zuly Elizabeth Jimenez Perez, Nam In Baek, Ramya Mathiyalagan, Josua Markus, Deok-Chun Yang   +8 more
doaj   +1 more source

Molecular Cloning and Functional Analysis of IrUGT86A1-like Gene in Medicinal Plant Isodon rubescens (Hemsl.) Hara

Life, 2022
The synthesis of secondary metabolites in plants often includes glycosylation modifications. Often, the final step of constructing plant secondary metabolites is completed by glycosylation transferases, which are also involved in many cell processes.
Conglong Lian, Jinxu Lan, Bao Zhang, Hao Yang, Kaihua Guo, Jingjing Li, Suiqing Chen   +6 more
doaj   +1 more source

UGT76B1 and 41 Additional Arabidopsis UDP-Glycosyltransferases Show No Detectable In Vitro Glycosylation Activity Toward N-Hydroxypipecolic Acid

Life
N-hydroxypipecolic acid (NHP) is a key mobile signal in systemic acquired resistance in plants, and its glycosylation has been proposed to regulate immune signaling. Previous studies have demonstrated that the UDP-glycosyltransferase UGT76B1, known as an
Jiyuan Bao, Taiga Uchiyama, Kazuki Kusunoki, Yuka Shinohara, Yurika Tanigawa, Megumi Watanabe, Nanami Sakata, Hidenori Matsui, Kazuhiro Toyoda, Yuki Ichinose, Yoshiteru Noutoshi   +10 more
doaj   +1 more source

Synthesis and Preliminary Evaluation of Biological Activity of Glycoconjugates Analogues of Acyclic Uridine Derivatives

Molecules, 2018
Herein we present the methodology for obtaining glycosyltransferase inhibitors, analogues of natural enzyme substrates of donor-type: UDP-glucose and UDP-galactose.
Roman Komor, Gabriela Pastuch-Gawolek, Ewelina Krol, Wieslaw Szeja   +3 more
doaj   +1 more source

NleB2 from enteropathogenic Escherichia coli is a novel arginine-glucose transferase effector.

PLoS Pathogens, 2021
During infection, enteropathogenic Escherichia coli (EPEC) and enterohaemorrhagic E. coli (EHEC) directly manipulate various aspects of host cell function through the translocation of type III secretion system (T3SS) effector proteins directly into the ...
Cristina Giogha, Nichollas E Scott, Tania Wong Fok Lung, Georgina L Pollock, Marina Harper, Ethan D Goddard-Borger, Jaclyn S Pearson, Elizabeth L Hartland   +7 more
doaj   +1 more source

Enhancing Meat Quality Through Dietary Fiber: Insights Into the Gut Microbiota‐Derived Short‐Chain Fatty Acids‐Muscle Axis

Animal Research and One Health, EarlyView.
Dietary fiber (DF) modulates meat quality through the gut‐muscle axis by reshaping gut microbiota, improving intestinal integrity, reducing inflammation, and regulating muscle metabolism. These mechanisms influence muscle fiber characteristics and post‐mortem biochemistry, thereby enhancing tenderness, juiciness, color, flavor, and nutritional value of
Zeshan Zulfiqar, Muhammad Arslan Asif, Layla Al‐Mitib, Nabeela Ashiq, Haojie Wang, Yalei Cui, Boshuai Liu, Shucheng Huang, Yinghua Shi   +8 more
wiley   +1 more source

Combining phenotypic, physiological, and multi‐omics studies to provide new insights on the role of 2,4‐epibrassinolide in regulation of seed shattering in Elymus sibiricus L.

Grassland Research, EarlyView.
Abstract Background Seed shattering limits the production of Elymus sibiricus L., and application of exogenous brassinosteroid significantly alleviates plant organ abscission. Methods To explore the potential regulatory network of brassinosteroid on seed shattering, the abscission zone of E. sibiricus cv. Lanyu No.
Huanhuan Lu, Yiyang Wan, Liuban Tang, Yongsen Qiu, Yancui Zhao, Yuying Zheng, Wengang Xie   +6 more
wiley   +1 more source

Study on Revealing Peanut-Related Disease Prevention Gene Clusters via Whole Transcriptome Sequencing

Agriculture, 2023
Root rot caused by Fusarium oxysporum is a serious root disease that affects the growth and yield of peanut crops worldwide. Identifying resistance genes is of great significance for both conventional and molecular breeding.
Hongbo Du, Nan Lu, Chuanrong Li
doaj   +1 more source