Results 141 to 150 of about 3,097 (169)
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In Vitro Cellular & Developmental Biology - Plant, 2000
Tissue-specific patterns and levels of gene expression were characterized in transgenic Gladiolus plants that contained the phosphinothricin acetyltransferase (bar)-β-glucuronidase (uidA) fusion gene under transcriptional control of the promoter from either the cauliflower mosaic virus 35S (CaMV 35S), duplicated CaMV 35S (2×CaMV 35S), rice actin (Act1),
Kathryn Kamo +2 more
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Tissue-specific patterns and levels of gene expression were characterized in transgenic Gladiolus plants that contained the phosphinothricin acetyltransferase (bar)-β-glucuronidase (uidA) fusion gene under transcriptional control of the promoter from either the cauliflower mosaic virus 35S (CaMV 35S), duplicated CaMV 35S (2×CaMV 35S), rice actin (Act1),
Kathryn Kamo +2 more
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Nucleotide sequence of a regulatory region of the uidA gene in Escherichia coli K12
Molecular and General Genetics MGG, 1985Multiple regulatory events are involved in the expression of the uidA gene. A regulatory region of this gene has been located on a 460 base pair Sau3A-EcoRI fragment and its nucleotide sequence was determined by the dideoxy method using pEMBL plasmids. A preliminary analysis of this sequence revealed the presence of numerous palindromic structures with
C, Blanco +2 more
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A change in the stability of marker nptII and uidA gene expression in transgenic tobacco plants
Russian Journal of Genetics, 2006A comparative analysis of variability of heterologous gene expression was conducted in original (T0) transgenic tobacco plants, differing with respect to the copy number of uidA gene (a single and two copies as a direct duplication) within the T-DNA insertion, and among progenies obtained by their self-pollination (T1).
T V, Marenkova, E V, Deĭneko
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Nucleotide sequence corrections of the uidA open reading frame encoding β-glucuronidase
Gene, 1994Part of the open reading frame of uidA, encoding beta-glucuronidase, was sequenced and two differences were found with the previously reported nucleotide sequence [Jefferson et al., Proc. Natl. Acad. Sci. USA 83 (1986) 8447-8451]. One is a silent mutation, the other results in the Glu279-->Gln substitution.
H R, Schlaman +3 more
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Detection of faecal pollution in water by an Escherichia coli uidA gene probe
Journal of Microbiological Methods, 1991Abstract This paper describes a method for the detection of faecal pollution in water, using a gene probe based on the Escherichia coli K-12 gene, uidA , which encodes β-glucuronidase (GUD). GUD has been reported to be produced by 97–99.5% of E. coli and we report that all E.
David H. Green +3 more
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uidA gene transfer and expression in maize microspores using the biolistic method
Protoplasma, 1995The ability to recover male gametophyte derived plants, which is necessary to get transformed haploid plants, was verified for a hybrid of maize. Using the isolated microspore culture technique, a 9 × 10−5 plant regeneration frequency was obtained. Maize microspores were bombarded with tungsten particles using a PDS He/1000 apparatus. GUS expression in
Jardinaud, M.F. +3 more
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Plant Science, 1994
Abstract Transient GUS expression levels in microprojectile bombarded, microspore-derived embryoids of bread wheat ( Triticum aestivum cv. Pavon) were evaluated when varying the stage of embryoid development, time in culture, type of promoter, and other physical parameters. GUS expression was histochemically measured 48 h after delivery of the uidA
Terry A. Loeb, Thomas L. Reynolds
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Abstract Transient GUS expression levels in microprojectile bombarded, microspore-derived embryoids of bread wheat ( Triticum aestivum cv. Pavon) were evaluated when varying the stage of embryoid development, time in culture, type of promoter, and other physical parameters. GUS expression was histochemically measured 48 h after delivery of the uidA
Terry A. Loeb, Thomas L. Reynolds
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Optimization of the uidA gene transfer into somatic embryos of rose via Agrobacterium tumefaciens
Plant Physiology and Biochemistry, 2002The effects of different concentrations of kanamycin (10-250 mg l –1 ), carbencillin (250 and 500 mg l –1 ), cefotaxime (250 and 500 mg l –1 ), and combinations of carbencillin and cefotaxime (each at 150 mg l –1 ) on regeneration from different tissues of rose ( Rosa hybrida cv.
Xiangqian Li +2 more
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Plant Cell, Tissue and Organ Culture, 1999
Transfer and expression of the β-glucuronidase gene (uidA) in cultured cotyledons of stone pine (Pinus pinea L.) was obtained by microprojectile bombardment. Conditions for optimum transient expression were established by using plasmid pBI121 delivered by 1.0 μm-diameter gold particles, into 1-day-old cultured cotyledons.
Jaime M. Humara +2 more
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Transfer and expression of the β-glucuronidase gene (uidA) in cultured cotyledons of stone pine (Pinus pinea L.) was obtained by microprojectile bombardment. Conditions for optimum transient expression were established by using plasmid pBI121 delivered by 1.0 μm-diameter gold particles, into 1-day-old cultured cotyledons.
Jaime M. Humara +2 more
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Transforming embryogenic cell lines of Gladiolus with either a bar-uidA fusion gene or cobombardment
In Vitro Cellular & Developmental Biology - Plant, 2000Embryogenic cell lines of Gladiolus were bombarded with the bar-uidA fusion gene under the cauliflower mosaic virus (CaMV) 35S promoter (pDM327) or cobombarded with uidA under the CaMV 35S promoter (pBCG) and bar under the CaMV 35S promoter (pDM307). Over 500 cell lines were isolated for either the fusion gene or cobombarded cells following selection ...
Kathryn Kamo +2 more
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