Osmotically balanced, large unilamellar liposomes that enable sustained bupivacaine release for prolonged pain relief in in vivo rat models [PDF]
Scientific Reports, 2021 To efficiently prolong analgesic effects, we developed osmotically balanced, large unilamellar liposomes (~ 6 μm in diameter) in which highly concentrated bupivacaine (up to 30 mg/mL) was encapsulated, and their sustained bupivacaine release was highly ...
Hyebin Yoo +3 more
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Encapsulation of protein/DNA complexes into unilamellar liposomes via annexin-mediated membrane recruitment and sonication [PDF]
Cell Reports: MethodsSummary: This paper reports an effective protocol to encapsulate native protein/DNA complexes into unilamellar vesicles composed of natural lipids without the use of organic solvents, in physiological buffers, and at low protein/DNA concentrations.
Michael Burger +3 more
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Preparation of unilamellar liposomes v1 [PDF]
, 2023 We present here a protocol for preparing liposomes that can be used to monitor binding of proteins to vesicles of various membrane curvatures. We used this to study the association of the PPM1H phosphatase with highly curved membranes due to its N-terminal amphipathic helix.
Ayan Adhikari
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Encapsulation of Hydrophobic Apigenin into Small Unilamellar Liposomes Coated with Chitosan Through Ethanol Injection and Spray Drying. [PDF]
Food Bioproc Tech, 2023 Ang SS, Thoo YY, Siow LF.
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Effect of ultrasound parameters for unilamellar liposome preparation [PDF]
Ultrasonics Sonochemistry, 2009 In this study, it was investigated the effects of ultrasound, namely power input, distance from ultrasound tip to base of reactor and treatment time, in the formation of liposomes. Results indicate a dependence on cavitation events that are a function of power input, and consequently dependent on the position of the probe within the reaction vessel and
Raquel Silva +3 more
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Efficient gene transfer using unilamellar fusogenic liposomes.
Drug Delivery System, 1994 We have developed fusogenic liposomes with virus envelope proteins on the surface. These liposomes can introduce their contents (DNA, RNA, and protein) into cell cytoplasm directly by membrane fusion in vitro and in vivo. The liposomes can fuse with cell membrane as efficient as virus particles and can maintain their activity indefinitely in liquid ...
Hiroyuki Mizuguchi +4 more
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Preparation of unilamellar fusogenic liposomes using the Sendai virus.
Folia Pharmacologica Japonica, 1998 We describe a method to prepare unilamellar fusogenic liposomes (FL) that can deliver their content directly into tissue cells and cancer cells of living animals as well as into cultured animal cells. The method consists of three important steps: 1) preparation of unilamellar liposomes encapsulating the molecules to be delivered: 2) preparation of the ...
Mahito Nakanishi, Hiroyuki Mizuguchi
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Ultrasonic evidence for structural relaxation in large unilamellar liposomes [PDF]
The Journal of the Acoustical Society of America, 1983 The ultrasonic absorption of large unilamellar vesicles (average diameter 0.2 μm) was determined in the frequency range 0.5–5 MHz. The liposomes were composed of a 4:1 mixture by weight of dipalmitoyl phosphatidylcholine and dipalmitoyl phosphatidylglycerol.
Philip R. Strom-Jensen +2 more
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Interaction of unilamellar liposomes with serum lipoproteins and apolipoproteins
Journal of Lipid Research, 1980 The effect of rat whole blood plasma, serum, serum lipoproteins, and apolipoproteins on the stability of unilamellar liposomes prepared with French pressure cell was evaluated by measuring the release of entrapped carboxyfluorescein and by electron ...
L S Guo +4 more
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A Systematic Preparation of Liposomes with Yerba Mate (Ilex paraguariensis) Extract [PDF]
PlantsLipid vesicles, liposomes and phytosomes have been gaining significant attention in various applications for phytochemical preservation. Furthermore, yerba mate (Ilex paraguariensis) contains a high content of bioactive compounds with functional ...
Yasmine Miguel Serafini Micheletto +3 more
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