Reprogramming natural proteins using unnatural amino acids. [PDF]
RSC Adv, 2021 Incorporation of unnatural amino acids into protein offers wide array of applications in fundamental and applied science.
Adhikari A +9 more
europepmc +4 more sources
Directed-evolution of translation system for efficient unnatural amino acids incorporation and generalizable synthetic auxotroph construction [PDF]
Nature Communications, 2021 In this paper, the authors report the directed-evolution of translation systems that allow the incorporation of unnatural amino acids with similar efficiency to natural amino acids and the construction of synthetic auxotroph in a generalizable way.
Hongxia Zhao +10 more
doaj +2 more sources
Toward efficient multiple-site incorporation of unnatural amino acids using cell-free translation system [PDF]
Synthetic and Systems Biotechnology, 2022 Amber suppression has been widely used to incorporate unnatural amino acids (UNAAs) with unique structures or functional side-chain groups into specific sites of the target protein, which expands the scope of protein-coding chemistry.
Jiaqi Hou +7 more
doaj +2 more sources
Efficient Incorporation of Unnatural Amino Acids into Proteins with a Robust Cell-Free System [PDF]
Methods and Protocols, 2019 Unnatural proteins are crucial biomacromolecules and have been widely applied in fundamental science, novel biopolymer materials, enzymes, and therapeutics.
Wei Gao, Ning Bu, Yuan Lu
doaj +2 more sources
Advances and Challenges in Cell-Free Incorporation of Unnatural Amino Acids Into Proteins [PDF]
Frontiers in Pharmacology, 2019 Incorporation of unnatural amino acids (UNAAs) into proteins currently is an active biological research area for various fundamental and applied science.
Wei Gao +7 more
doaj +2 more sources
Editorial: Expansion of the Genetic Code: Unnatural Amino Acids and their Applications [PDF]
Frontiers in Chemistry, 2022 Subhendu Sekhar Bag +2 more
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Frontiers in Molecular Biosciences, 2022 Amino acid sequences of proteins are encoded in nucleic acids composed of four letters, A, G, C, and T(U). However, this four-letter alphabet coding system limits further functionalities of proteins by the twenty letters of amino acids.
Michiko Kimoto, Ichiro Hirao
doaj +1 more source
An expanded set of amino acid analogs for the ribosomal translation of unnatural peptides. [PDF]
PLoS ONE, 2007 The application of in vitro translation to the synthesis of unnatural peptides may allow the production of extremely large libraries of highly modified peptides, which are a potential source of lead compounds in the search for new pharmaceutical agents ...
Matthew C T Hartman +3 more
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Development and Testing of Force Field Parameters for Phenylalanine and Tyrosine Derivatives
Frontiers in Molecular Biosciences, 2020 Theoretical analyses are valuable for the exploration of the effects of unnatural amino acids on enzyme functions; however, many necessary parameters for unnatural amino acids remain lacking.
Xiaowen Wang, Xiaowen Wang, Wenjin Li
doaj +1 more source
Molecules, 2022 The hybrid peptides consisting of α and β-amino acids show great promise as peptidomimetics that can be used as therapeutic agents. Therefore, the development of new unnatural amino acids and the methods of their incorporation into the peptide chain is ...
Urszula Bąchor +3 more
doaj +1 more source