Results 111 to 120 of about 107,812 (233)

Resolution and Quantitation of Ribonucleosides and Deoxyribonucleosides in Digested Genomic DNA by UHPLC–MS/MS

Biomedical Chromatography, Volume 40, Issue 10, October 2026.
ABSTRACT Deoxyuridine (dUrd) is often misincorporated into genomic DNA in cancer patients receiving antimetabolite chemotherapy. This activates base excision repair and recruits ataxia telangiectasia‐mutated and Rad3‐related (ATR), which coordinates DNA repair with nucleotide metabolism by facilitating ribonucleotide reductase (RNR) activity ...
Joshua J. Deppas, Reyna Jones, Robert A. Parise, Pinakin Pandya, Christopher J. Bakkenist, Jan H. Beumer   +5 more
wiley   +1 more source

Neq2X7: a multi-purpose and open-source fusion DNA polymerase for advanced DNA engineering and diagnostics PCR

BMC Biotechnology
Thermostable DNA polymerases, such as Taq isolated from the thermophilic bacterium Thermus aquaticus, enable one-pot exponential DNA amplification known as polymerase chain reaction (PCR). However, properties other than thermostability - such as fidelity,
Cristina Hernández-Rollán, Anja K. Ehrmann, Arsenios Vlassis, Vijayalakshmi Kandasamy, Morten H. H. Nørholm   +4 more
doaj   +1 more source

Receptors responsive to extracellular pyrimidine nucleotides.

, 1997
Extracellular adenine nucleotides have long been known to mediate pharmacological responses via several subtypes of P2 purinoceptors. More recently, however, similar responses to uracil nucleotides have been demonstrated.
Boeynaems, Jean-Marie, Communi, Didier
core  

A bacterial protein inhibitor of dUTPase disrupts zebrafish embryogenesis through a conserved active‐site mechanism

Protein Science, Volume 35, Issue 10, October 2026.
Abstract Deoxyuridine triphosphatase (dUTPase) is essential for DNA replication fidelity and embryonic development in metazoa, yet whether a protein inhibitor can acutely disrupt dUTPase function in a living vertebrate has not been tested. The Staphylococcus aureus Stl protein inhibits diverse dUTPases in vitro, but its efficacy in a eukaryotic ...
Viktória Perey‐Simon, Zoé Sára Tóth, Dániel Dombovári, Latifa Kazzazy, Máté Varga, Beáta G. Vértessy, Gergely Nándor Nagy, Kinga Nyíri   +7 more
wiley   +1 more source

Loss of DNA methylation is not associated with venetoclax–hypomethylating agents synergy in acute myeloid leukaemia cells

British Journal of Haematology, EarlyView.
Aida Selimović‐Pašić, Lisa Haglund, Maike Bensberg, Júlia Goldmann, Padraig D'arcy, Sandra Hellberg, Colm E. Nestor   +6 more
wiley   +1 more source

Comparing the Performance of Double‐Stranded and Single‐Stranded DNA Libraries for Ancient Oral Microbiome Reconstruction

Molecular Ecology Resources, Volume 26, Issue 7, October 2026.
ABSTRACT DNA library construction methods can affect the recovery of ancient DNA, thus influencing downstream analyses. While single‐stranded library preparation (ssLib) has been shown to outperform double‐stranded (dsLib) for highly degraded vertebrate host DNA, especially for samples older than 40,000 years, few studies have examined how library ...
Keri Burge, Irina M. Velsko, Domingo C. Salazar‐García, María Haber Uriarte, Joaquín Lomba Maurandi, Christina Warinner   +5 more
wiley   +1 more source

YIPFα1A expression is regulated by multilayered molecular mechanisms

FEBS Open Bio, Volume 16, Issue 9, Page 1669-1690, September 2026.
YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji, Yurika Nakanishi, Nobuhiro Nakamura   +2 more
wiley   +1 more source

Ribosylurea accumulates in yeast urc4 mutants.

, 2010
Yeast Saccharomyces (Lachancea) kluyveri urc4 mutants, unable to grow on uracil, biotransformed (14)C(2)-uracil into two labeled compounds, as detected by high performance liquid chromatography (HPLC). These two compounds could also be obtained following
G. Andersen, Domkin, Vladimir,, Rasmussen, Anna, V. Domkin, Björnberg, O, Vodnala, Munender,, A. Hofer, M. Vodnala, Andersen, G, J. Piškur, Piskur, J, A. Rasmussen, Piskur, Jure, Hofer, Anders,, Björnberg, Olof, Vodnala, M, O. Björnberg, Hofer, A, Domkin, V, Rasmussen, A   +19 more
core   +1 more source

CREAT: A CRISPR‐Based Genome Trimming Strategy for Systematic Identification of Dispensable Regions and Rapid Genome Reduction

Advanced Science, Volume 13, Issue 50, 7 September 2026.
Current genome‐reduction methods work in a trial‐and‐error fashion, constituting a long‐standing bottleneck in the research. We developed CREAT (CRISPR‐based genome trimming with a multi‐homology‐arm template), a CRISPR‐based genetic approach that enables systematically classifying essential versus non‐essential genomic regions and subsequently ...
Guanhua Yuan, Zhe Gao, Yingxuan Qi, Yang Zhang, Xuhui Tian, Pengpeng Zhao, Xu Feng, Qunxin She   +7 more
wiley   +1 more source

Synergistic HMGN1 and VP64 Fusions Potentiate High‐Precision and PAM‐Flexible Base Editing

Advanced Science, Volume 13, Issue 50, 7 September 2026.
A novel CDA1Δ‐SpRY architecture fused with HMGN1 and VP64 yields a nearly PAM‐less base editing platform. By focusing cytosine conversion predominantly at position −18, this synergistic complex ensures highly precise targeting. Demonstrating enhanced efficiency across diverse models, including yeast and rice, the platform offers a robust solution for ...
Xi Luo, Yuyao Qu, Zhengyan Ye, Zheng Li, Yuanyan Zhang, Longjiong Luo, Shaokang Li, Wei Zhao, Ming Wang, Ralph Bock, Jianmin Wan, Junjie Tan   +11 more
wiley   +1 more source