Results 91 to 100 of about 107,812 (233)

Adipocyte purinergic receptors activated by uracil nucleotides as obesity and type 2 diabetes targets. [PDF]

Curr Opin Pharmacol, 2022
Jain S, Jacobson KA.
europepmc   +1 more source

The continuing significance of chiral agrochemicals

Pest Management Science, Volume 81, Issue 4, Page 1697-1716, April 2025.
In the time frame 2018–2023, around 43% of the 35 chiral agrochemicals introduced to the market (herbicides, fungicides, insecticides, acaricides, and nematicides) contain one or more stereogenic centers in the molecule, and almost 69% of them have been marketed as racemic mixtures of enantiomers or stereoisomers.
Peter Jeschke
wiley   +1 more source

Increasing Cytosine Base Editing Scope and Efficiency With Engineered Cas9-PmCDA1 Fusions and the Modified sgRNA in Rice

Frontiers in Genetics, 2019
Base editors that do not require double-stranded DNA cleavage or homology-directed repair enable higher efficiency and cleaner substitution of targeted single nucleotides in genomic DNA than conventional approaches.
Ying Wu, Wen Xu, Feipeng Wang, Si Zhao, Feng Feng, Jinling Song, Chengwei Zhang, Jinxiao Yang   +7 more
doaj   +1 more source

Comparative Analysis of Stress Adaptation in the Yeast Microbiome of Cactus

Yeast, EarlyView.
Yeasts and related fungi isolated from cacti or nearby non‐cactus plants were characterized phenotypically and genomically, revealing differences that hint at modes of adaptation to the cactus host. ABSTRACT Together with other fungi, yeasts make up a significant component of the plant microbiome. As the planet warms, cacti expand their range.
Alya N. Hussain, Kevin T. Fotso, Michael A. McMurray   +2 more
wiley   +1 more source

Expedient single-round selection of hyper-modified aptamer targeting insulin receptor from over-represented dually nucleobase-modified DNA libraries

Nature Communications
Discovery of functional nucleic acids from randomized libraries typically relies on multiple, time-consuming iterative rounds of in vitro selection with low success rate.
Pablo Alberto Franco-Urquijo, Marek Ondruš, Jaroslav Kurfürst, Jana Škerlová, Irena Selicharová, Lucie Mužíková Čechová, Hana Šváchová, Alena Semerádtová, Anatolij Filimoněnko, Adéla Fejfarová, Jiří Homola, Tomáš Kouba, Michal Hocek   +12 more
doaj   +1 more source

Yeast Knockout Strain Collection: Driving Functional Genomics, Biotechnological Applications and Human Disease Research

Yeast, EarlyView.
ABSTRACT The knockout strain collection of Saccharomyces cerevisiae has served as a valuable resource for functional genomics and yeast‐based biotechnology studies. A comprehensive single‐gene knockout strain collection, covering nearly all non‐essential genes, together with complementary mutant collections for essential genes, including temperature ...
Takashi Hirasawa
wiley   +1 more source

Targeted rapid amplification of cDNA ends (T-RACE)-an improved RACE reaction through degradation of non-target sequences [PDF]

, 2010
Amplification of the 5' ends of cDNA, although simple in theory, can often be difficult to achieve. We describe a novel method for the specific amplification of cDNA ends. An oligo-dT adapter incorporating a dUTP-containing PCR primer primes first-strand
Bower, Neil I., I. A. Johnston, N. I. Bower, Johnston, Ian A.   +3 more
core   +1 more source

Changes of Microbial and Component Composition of Giraffe Milk During Mid‐ to Late Lactation

Zoo Biology, EarlyView.
Giraffe milk – graphical abstract text, Giraffe: late lactation milk, Involution at approximately 15 months of lactation, Content of saccharides and fat decrease, Protein content increases, Content of minerals increase, Microbiological quality high ABSTRACT The proximate, mineral and microbiological composition of milk from five giraffes from mid ...
G. Osthoff, C. J Hugo, S Van der Merwe, A Hugo, F Deacon   +4 more
wiley   +1 more source

Nucleic Acid Ligands With Protein-like Side Chains: Modified Aptamers and Their Use as Diagnostic and Therapeutic Agents

Molecular Therapy: Nucleic Acids, 2014
Limited chemical diversity of nucleic acid libraries has long been suspected to be a major constraining factor in the overall success of SELEX (Systematic Evolution of Ligands by EXponential enrichment).
John C Rohloff, Amy D Gelinas, Thale C Jarvis, Urs A Ochsner, Daniel J Schneider, Larry Gold, Nebojsa Janjic   +6 more
doaj   +1 more source

Transgenic selection and underlying mechanisms in apicomplexan parasites

The FEBS Journal, EarlyView.
Transgenic selection markers have driven genetic engineering in apicomplexans, enabling precise, iterative experiments. This review discusses mechanistic details of drug selection markers, strategies for marker recycling, and practical considerations for several clinically relevant parasites.
Swaroop Peddiraju, Rakhee Lohia, Nishith Gupta   +2 more
wiley   +1 more source