Results 71 to 80 of about 669,704 (210)

Coupling of both a transactivation module and a double‐stranded DNA‐binding domain boosts Cas12i3 variant‐based cytosine and adenine editing in plants

Journal of Integrative Plant Biology, EarlyView.
Fusions of a transactivation module and a double‐stranded DNA‐binding domain produced effective Cas12i3 variant‐based cytosine and adenine base editors, with improved C‐to‐T and A‐to‐G base editing efficiency and expanded editing window. Generated herbicide‐resistant rice germplasm by using these base editors demonstrates their potential for precise ...
Chen Zhang, Jingying Li, Yucai Li, Lei Yan, Christina Seok Yien Yong, Shaoya Li, Yubing He, Lanqin Xia   +7 more
wiley   +1 more source

Stress-Induced Low Complexity RNA Activates Physiological Amyloidogenesis

Cell Reports, 2018
Summary: Amyloid bodies (A-bodies) are inducible membrane-less nuclear compartments composed of heterogeneous proteins that adopt an amyloid-like state. A-bodies are seeded by noncoding RNA derived from stimuli-specific loci of the rDNA intergenic spacer
Miling Wang, Xianzun Tao, Mathieu D. Jacob, Clayton A. Bennett, J.J. David Ho, Mark L. Gonzalgo, Timothy E. Audas, Stephen Lee   +7 more
doaj   +1 more source

Enhancing CRISPR‐Cas12a base editing in plants with LbCas12a variants and introns

Journal of Integrative Plant Biology, EarlyView.
Intron optimization of LbCas12a‐RRV improves cytosine and adenine base editing efficiency in plants, supports multiplexed and double‐strand break‐free genome modification, and expands precise genome engineering tools for crop breeding and plant functional research. ABSTRACT Cytosine base editors (CBEs) and adenine base editors (ABEs) are powerful tools
Yanhao Cheng, Gen Li, Man Zhou, Rushil Mandlik, Doris Wang, Yiping Qi   +5 more
wiley   +1 more source

Divergent roles of ent‐kaurene oxidase paralogs in rice momilactone biosynthesis

Journal of Integrative Plant Biology, EarlyView.
Rice contains a five‐gene tandem array of genes encoding members of the ent‐kaurene oxidase (KO) family, with OsKOL4 and OsKOL5 playing divergent roles in momilactone metabolism, but both acting in the production of antifungal and antibacterial phytoalexins, which are important for disease resistance.
Zhibiao Wang, Yiling Feng, Leiming You, Meimei Xu, Kristin Helwig, Riqing Li, Si Nian Char, Bing Yang, Reuben J. Peters   +8 more
wiley   +1 more source

Adipocyte purinergic receptors activated by uracil nucleotides as obesity and type 2 diabetes targets. [PDF]

Curr Opin Pharmacol, 2022
Jain S, Jacobson KA.
europepmc   +1 more source

Structure-Activity Relationship of Purine and Pyrimidine Nucleotides as Ecto-5′-Nucleotidase (CD73) Inhibitors

Journal of Medicinal Chemistry, 2019
CD73 converts AMP to immunosuppressive adenosine, and its inhibition was proposed as a new strategy for cancer treatment. We synthesized 5′-O-[(phosphonomethyl)phosphonic acid] derivatives of purine and pyrimidine nucleosides, which represent nucleoside ...
Anna Junker, Christian Renn, Clemens Dobelmann, V. Namasivayam, Shanu Jain, K. Losenkova, H. Irjala, Sierra Duca, R. Balasubramanian, Saibal Chakraborty, Frederik Börgel, H. Zimmermann, G. Yegutkin, C. Müller, K. Jacobson   +14 more
semanticscholar   +1 more source

Optimized TadA‐derived base editors efficiently manipulate mRNA splicing by A‐to‐G and C‐to‐K editing in potato

Journal of Integrative Plant Biology, EarlyView.
Two optimized TadA‐derived base editors efficiently generate diverse splicing variants by targeting specific splice sites in potato. ABSTRACT Pre‐messenger RNA (pre‐mRNA) splicing is a critical mechanism for post‐transcriptional regulation in plants. Through alternative splicing, plants produce diverse transcriptomes and proteomes that finely regulate ...
Kaiyuan Chen, Yan Zhang, Jiuzhou Deng, Lumin Zhang, Huiying Zhou, Yaxin Duan, Enle Xiao, Guangtao Zhu, Chunzhi Zhang   +8 more
wiley   +1 more source

Increasing Cytosine Base Editing Scope and Efficiency With Engineered Cas9-PmCDA1 Fusions and the Modified sgRNA in Rice

Frontiers in Genetics, 2019
Base editors that do not require double-stranded DNA cleavage or homology-directed repair enable higher efficiency and cleaner substitution of targeted single nucleotides in genomic DNA than conventional approaches.
Ying Wu, Wen Xu, Feipeng Wang, Si Zhao, Feng Feng, Jinling Song, Chengwei Zhang, Jinxiao Yang   +7 more
doaj   +1 more source

A cytochrome P450 gene, GmSUR2a, confers submergence tolerance and improves yield in soybean by modulating auxin homeostasis

Journal of Integrative Plant Biology, EarlyView.
The key cytochrome P450 gene GmSUR2a enables soybeans to withstand destructive submergence stress. By lowering the level of the plant hormone indole‐3‐acetic acid, this gene improves soybean survival and increases field yield. It offers an important tool for breeding stress‑resilient soybeans, securing food production against extreme weather conditions.
Yangyang Chen, Pingzhang Feng, Mengwei Zheng, Lipei Zhao, Yi Li, Yali Jing, Yisa Wang, Bo Zhang, Changzhong Liu, Chengyu Wei, Jijun Yan, Jinfang Chu, Shanshan Chu, Erhui Xiong, Yongqing Jiao   +14 more
wiley   +1 more source

Expedient single-round selection of hyper-modified aptamer targeting insulin receptor from over-represented dually nucleobase-modified DNA libraries

Nature Communications
Discovery of functional nucleic acids from randomized libraries typically relies on multiple, time-consuming iterative rounds of in vitro selection with low success rate.
Pablo Alberto Franco-Urquijo, Marek Ondruš, Jaroslav Kurfürst, Jana Škerlová, Irena Selicharová, Lucie Mužíková Čechová, Hana Šváchová, Alena Semerádtová, Anatolij Filimoněnko, Adéla Fejfarová, Jiří Homola, Tomáš Kouba, Michal Hocek   +12 more
doaj   +1 more source