Results 141 to 150 of about 22,593 (200)

Organocatalytic Switches of DNA Glycosylase OGG1 Catalyze a Highly Efficient AP‐Lyase Function

Chemistry – A European Journal, Volume 31, Issue 33, June 12, 2025.
Organocatalytic switches are small molecules that combine active site affinity and a nitrogen‐centered base for catalysis, effectively removing the rate‐determining step of the OGG1 biochemical cascade. Here, we identify a minimal structure and modulate the active nitrogen pKa, enabling the catalysis of ultrafast AP site incisions in a range of pH ...
Mario Kehler, Kaixin Zhou, Aurino M. Kemas, Alicia del Prado, Emma Scaletti Hutchinson, Elinor Hesslefors Nairn, Marek Varga, Yvonne Plattner, Yi Zhong, Oryn Purewal‐Sidhu, James Haslam, Elisée Wiita, Heather Gildie, Karolina Singerova, Zuzanna Szaruga, Ingrid Almlöf, Femke M. Hormann, Kang‐Cheng Liu, Olov Wallner, Florian Ortis, Evert J. Homan, Opher Gileadi, Sean G. Rudd, Pål Stenmark, Miguel de Vega, Thomas Helleday, Nicholas D. D'Arcy‐Evans, Volker M. Lauschke, Maurice Michel   +28 more
wiley   +1 more source

Sequencing accuracy and systematic errors of nanopore direct RNA sequencing

BMC Genomics
Background Direct RNA sequencing (dRNA-seq) on the Oxford Nanopore Technologies (ONT) platforms can produce reads covering up to full-length gene transcripts, while containing decipherable information about RNA base modifications and poly-A tail lengths.
Wang Liu-Wei, Wiep van der Toorn, Patrick Bohn, Martin Hölzer, Redmond P. Smyth, Max von Kleist   +5 more
doaj   +1 more source

Chemical Switching: A Concept Inspired by Strategies from Biocatalysis and Organocatalysis

ChemBioChem, Volume 26, Issue 11, June 3, 2025.
Chemical Switching is introduced as a novel technology that exploits the promiscuity of enzymatic transformations by applying small molecule organocatalytic switches (ORCAs) or gain‐of‐function protein variants. The modulation of underlying chemical principles thereby amplifies low‐rate or installs otherwise absent enzymatic activities to dominate ...
Torkild Visnes, Kaixin Zhou, Aurino M. Kemas, Dominic Campopiano, Volker M. Lauschke, Maurice Michel   +5 more
wiley   +1 more source

Organic Transistor‐Based Biosensors for Viral RNA Detection and Variant Monitoring: From SARS‐CoV‐2 to Effective Management of Future Pandemics

Advanced Sensor Research, Volume 4, Issue 6, June 2025.
A novel approach for direct sensing of viral RNA is proposed, allowing PCR‐free detection of viral RNA from genomic material, and variants screening by discrimination of polymorphic amplicons. The device is a cost‐effective, user‐friendly platform suitable for both mass screening kits and as a laboratory tool for an optimized management of resources ...
Stefano Lai, Roberto Cusano, Alessandra Scano, Giorgio Fotia, Annalisa Bonfiglio   +4 more
wiley   +1 more source

Assessment and Mitigation of CRISPR‐Cas9‐Induced Nontargeted Translocations

Advanced Science, Volume 12, Issue 21, June 5, 2025.
Various inverted repeat elements are dispersed throughout genomes. This study reveals that these elements can cause significant chromosomal rearrangements when CRISPR editing occurs in their proximity. The risk can be mitigated by incorporating inverted repeat‐homologous segments into the CRISPR system, which represents a promising strategy for ...
Zhiyang Hou, Qiyi Yi, Mengying Wu, Lijun Wu, Fanghua Li, Ting Wang, Po Bian   +6 more
wiley   +1 more source

Targeting PTBP3‐Mediated Alternative Splicing of COX11 Induces Cuproptosis for Inhibiting Gastric Cancer Peritoneal Metastasis

Advanced Science, Volume 12, Issue 21, June 5, 2025.
The splicing factor PTBP3 promotes COX11 exon skipping, allowing gastric cancer organoids to evade cuproptosis. Antisense oligonucleotide drugs targeting PTBP3‐mediated COX11 alternative splicing, in combination with copper ionophores, promote cuproptosis in organoids, thereby providing a therapeutic approach for gastric cancer peritoneal metastasis ...
Yajing Zhou, Chao Dong, Xiaochun Shen, Pengbo Wang, Tao Chen, Weikang Li, Xiaotong Sun, Peiyuan Li, Chengxiang Xu, Kaipeng Duan, Dongbao Li, Jin Zhou   +11 more
wiley   +1 more source

Exploring 2D Graphene‐Based Nanomaterials for Biomedical Applications: A Theoretical Modeling Perspective

Small Science, Volume 5, Issue 6, June 2025.
Two‐dimensional graphene‐based nanomaterials are of significant interest in biomedical applications due to their unique physicochemical properties. Computational modeling provides useful insights into their structure and interactions with biological systems, aiding the nanoparticle design.
Alexa Kamboukos, Nevena Todorova, Irene Yarovsky   +2 more
wiley   +1 more source

piRNA processing within non‐membrane structures is governed by constituent proteins and their functional motifs

The FEBS Journal, Volume 292, Issue 11, Page 2715-2736, June 2025.
The silencing of transposable elements is mediated by piRNAs through post‐transcriptional and transcriptional regulation. In Drosophila, piRNAs are processed within unique non‐membrane structures: nuage in germline cells and Yb bodies in somatic gonadal cells.
Ritsuko Suyama, Toshie Kai
wiley   +1 more source

Highly efficient Agrobacterium rhizogenes‐mediated gene editing system in Salvia miltiorrhiza inbred line bh2‐7

Plant Biotechnology Journal, Volume 23, Issue 6, Page 2406-2417, June 2025.
Summary The CRISPR/Cas9 system is a powerful tool for genomic editing with significant potential for gene function validation and molecular breeding in medicinal plants. Salvia miltiorrhiza, a model medicinal plant, was among the pioneers to utilize CRISPR/Cas9 technology, though achieving high‐efficiency homozygous knockout mutants has been ...
Mei Tian, Linglong Luo, Baolong Jin, Jianing Liu, Tong Chen, Jinfu Tang, Ye Shen, Haiyan Zhang, Juan Guo, Huawei Zhang, Guanghong Cui, Luqi Huang   +11 more
wiley   +1 more source

Elevated expression of SaMTP8.1 is involved in internal Mn detoxification in the hyperaccumulating ecotype of Sedum alfredii

The Plant Journal, Volume 122, Issue 5, June 2025.
SUMMARY Sedum alfredii (Sa) is known as a Cd/Zn hyperaccumulator, which usually grows in soil with high Mn in its natural habitat. However, it is unclear how S. alfredii copes with high Mn at both physiological and molecular levels. In this study, we characterized the Mn accumulation and tolerance in the hyperaccumulating ecotype (HE) of S. alfredii by
Jun Ge, Lingli Lu, Jian Feng Ma
wiley   +1 more source