Results 21 to 30 of about 601,747 (289)

Turning On Uracil-DNA Glycosylase Using a Pyrene Nucleotide Switch [PDF]

Journal of Biological Chemistry, 2001
Base flipping is a highly conserved process by which enzymes swivel an entire nucleotide from the DNA base stack into their active site pockets. Uracil DNA glycosylase (UDG) is a paradigm enzyme that uses a base flipping mechanism to catalyze the hydrolysis of the N-glycosidic bond of 2'-deoxyuridine (2'-dUrd) in DNA as the first step in uracil base ...
Yu Jiang, Keehwan Kwon, James T. Stivers
openalex   +3 more sources

Real-time PCR method for detection of short DNA using a deoxyuridine probe and application for detection of fomivirsen

BioTechniques, 2022
This study sought to develop a short DNA detection method using a deoxyuridine probe and polymerase chain reaction. The probe was hybridized to the target short DNA, which was then extended by DNA polymerase.
Naoki Harikai, Yuko Tanaka, Satoshi Miyashita, Kazumasa Zaima, Kazufusa Shinomiya   +4 more
doaj   +1 more source

Clay Catalysis of Oligonucleotide Formation: Kinetics of the Reaction of the 5'-Phosphorimidazolides of Nucleotides with the Non-Basic Heterocycles Uracil and Hypoxanthine [PDF]

Origins of life and evolution of the biosphere, 1999
K. Kawamura, J. Ferris
semanticscholar   +2 more sources

Involvement of pyrimidinoceptors in the regulation of cell functions by uridine and by uracil nucleotides.

TIPS - Trends in Pharmacological Sciences, 1989
R. Seifert, G. Schultz
semanticscholar   +2 more sources

On the prebiotic selection of nucleotide anomers: A computational study

Heliyon, 2022
Present-day known predominance of the β- over the α-anomers in nucleosides and nucleotides emerges from a thermodynamic analysis of their assembly from their components, i.e. bases, sugars, and a phosphate group.
Lázaro A.M. Castanedo, Chérif F. Matta
doaj   +1 more source

Biochemical characterization of uracil phosphoribosyltransferase from Mycobacterium tuberculosis. [PDF]

PLoS ONE, 2013
Uracil phosphoribosyltransferase (UPRT) catalyzes the conversion of uracil and 5-phosphoribosyl-α-1-pyrophosphate (PRPP) to uridine 5'-monophosphate (UMP) and pyrophosphate (PP(i)). UPRT plays an important role in the pyrimidine salvage pathway since UMP
Anne Drumond Villela, Rodrigo Gay Ducati, Leonardo Astolfi Rosado, Carlos Junior Bloch, Maura Vianna Prates, Danieli Cristina Gonçalves, Carlos Henrique Inacio Ramos, Luiz Augusto Basso, Diogenes Santiago Santos   +8 more
doaj   +1 more source

Studies on Nucleosides and Nucleotides. IX. Nucleophilic Substitution of Secondary Sulfonyloxy Groups of Pyrimidine Nucleosides. II. Reaction of 2, 2'-Anhydro-1-(3'-O-tosyl-β-D-arabinofuranosyl) uracil with Sodium Bromide, Sodium Ethanethiol, and Sodium Azide

, 1968
M. Hirata
semanticscholar   +3 more sources

Prior metabolite extraction fully preserves RNAseq quality and enables integrative multi-‘omics analysis of the liver metabolic response to viral infection

RNA Biology, 2023
Here, we provide an in-depth analysis of the usefulness of single-sample metabolite/RNA extraction for multi-‘omics readout. Using pulverized frozen livers of mice injected with lymphocytic choriomeningitis virus (LCMV) or vehicle (Veh), we isolated RNA ...
Zachary B Madaj, Michael S. Dahabieh, Vijayvardhan Kamalumpundi, Brejnev Muhire, J. Pettinga, Rebecca A. Siwicki, Abigail E. Ellis, Christine Isaguirre, Martha L. Escobar Galvis, Lisa DeCamp, Russell G. Jones, Scott A. Givan, Marie Adams, Ryan D. Sheldon   +13 more
doaj   +1 more source

Generation of single-nucleotide repair patches following excision of uracil residues from DNA. [PDF]

Molecular and Cellular Biology, 1992
The extent and location of DNA repair synthesis in a double-stranded oligonucleotide containing a single dUMP residue have been determined. Gently prepared Escherichia coli and mammalian cell extracts were employed for excision repair in vitro.
Grigory L. Dianov, A. Price, Tomas Lindahl   +2 more
openalex   +4 more sources

Silencing NTPDase3 activity rehabilitates the osteogenic commitment of post-menopausal stem cell bone progenitors

Stem Cell Research & Therapy, 2023
Background Endogenously released adenine and uracil nucleotides favour the osteogenic commitment of bone marrow-derived mesenchymal stromal cells (BM-MSCs) through the activation of ATP-sensitive P2X7 and UDP-sensitive P2Y6 receptors.
José Bernardo Noronha-Matos, Rui Pinto-Cardoso, Catarina Bessa-Andrês, Maria Teresa Magalhães-Cardoso, Fátima Ferreirinha, Maria Adelina Costa, José Marinhas, Rolando Freitas, Rui Lemos, Adélio Vilaça, António Oliveira, Julie Pelletier, Jean Sévigny, Paulo Correia-de-Sá   +13 more
doaj   +1 more source