Results 31 to 40 of about 4,673 (179)

Intelligent design of artificial biocatalyst for biomedical diseases

Journal of Intelligent Medicine, EarlyView.
This review summarizes recent advances in the intelligent design of artificial biocatalysts for biomedical diseases. By leveraging tailored design strategies, including environment‐responsive engineering and rational/artificial intelligence‐aided optimization, these biocatalysts enable precise modulation of pathological microenvironments and targeted ...
Lijie Zhang, Nan Song, Huanhuan Qiao, Si Sun   +3 more
wiley   +1 more source

Genetically Encoded Green Fluorescent Biosensors for Monitoring UDP-GlcNAc in Live Cells

, 2021
Uridine diphosphate N-acetylglucosamine (UDP-GlcNAc) is a nucleotide sugar used by glycosyltransferases to synthesize glycoproteins, glycosaminoglycans, glycolipids, and glycoRNA.
Hui-wang Ai (1645879), Zefan Li (3323217), Jing Zhang (23775)   +2 more
core   +1 more source

Identification of endoplasmic reticulum proteins involved in glycan assembly: synthesis and characterization of P3-(4-azidoanilido)uridine 5′-triphosphate, a membrane-topological photoaffinity probe for uridine diphosphate-sugar binding proteins [PDF]

Biochemical Journal, 1998
Much of the enzymic machinery required for the assembly of cell surface carbohydrates is located in the endoplasmic reticulum (ER) of eukaryotic cells. Structural information on these proteins is limited and the identity of the active polypeptide(s) is generally unknown.
D M, Rancour, A K, Menon
openaire   +2 more sources

Review of Congenital Myasthenic Syndrome Caused by Pathogenic Variants in GFPT1

Muscle &Nerve, EarlyView.
ABSTRACT Glutamine:fructose‐6‐phosphate transaminase 1 (GFPT1) catalyzes the first and rate‐limiting step of the hexosamine biosynthetic pathway (HBP) to generate UDP‐GlcNAc. GFPT1 exon 9 is specifically spliced in in striated muscles, which makes a long isoform of GFPT1 (GFPT1‐L).
Kinji Ohno, Mohammad Nazim, Ruchen Zhang, Paniz Farshadyeganeh, Mikako Ito, Bisei Ohkawara   +5 more
wiley   +1 more source

Chemical synthesis and isolation of UDP-2-deoxy glucose and galactose

, 2016
2-Deoxy sugars are attractive compounds in synthetic chemistry with regard to reactivity and stereoselectivity. Moreover, their ability to inhibit enzymes and metabolism is significant in biology.
Kenta Kurimoto (2519545), Shunya Takeuchi (3090003), Sanami Toyama (3089997), Shinji Itoda (3090000), Yukishige Ito (249123), Atsushi Miyagawa (39331), Hatsuo Yamamura (2518819)   +6 more
core   +1 more source

Substrate promiscuities of a bacterial galactokinase and a glucose-1-phosphate uridyltransferase enable xylose salvaging

, 2022
Galactokinases (GalKs) are structurally conserved proteins that exist in all domains of life. These enzymes catalyse the transfer of a phosphate group from adenosine triphosphate (ATP) to the anomeric hydroxyl group of galactose and show only negligible ...
Liu, Li, Voglmeir, Josef, Widmalm, Göran,, Shi, Jin-Min, Zhou, Hui, Wu, Ting-Ting, Zhang, Yao-Yao   +6 more
core   +1 more source

Comparison of Three Sympatric Desert Lizards: Digestive Tract Structure, Digestive Enzyme Activities, Gut Microbiota, and Metabolites

Integrative Zoology, EarlyView.
We performed 16S rDNA sequencing and metabolite profiling for three sympatric lizard species—Teratoscincus roborowskii, Phrynocephalus axillaris, and Eremias roborowskii—and compared their goblet cell and enzyme activities in the digestive tract. Our study suggests that the dietary niche may promote divergence or convergence of microbiota across host ...
Yi Yang, Ziyi Wang, Ruichen Wu
wiley   +1 more source

Engineered Saccharomyces cerevisiae as a biosynthetic platform of nucleotide sugars and heterologous glycosylation

, 2023
Glycosylation is a vital modification found all over biology. It can affect both structure and function of biomolecules as well as solubility and stability. Despite its ubiquity, glycosylation is still vastly understudied.
Crowe, Samantha Aiko
core  

Mutants of Escherichia coli K-12 “Cryptic,” or Deficient in 5′-Nucleotidase (Uridine Diphosphate-Sugar Hydrolase) and 3′-Nucleotidase (Cyclic Phosphodiesterase) Activity [PDF]

Journal of Bacteriology, 1973
Mutants of Escherichia coli have been selected for the absence of 5′-nucleotidase (uridine diphosphate-sugar hydrolase) and 3′-nucleotidase (2′,3′-cyclic phophodiesterase). Mutants selected for the absence of 5′-nucleotidase are of two kinds: those that lack detectable activity for the enzyme (Ush −
I R, Beacham, R, Kahana, L, Levy, E, Yagil   +3 more
openaire   +2 more sources

Metabolome Diversity Enhances Resistance of Intertidal Clams to Thermal Stress

Integrative Zoology, EarlyView.
Meretrix species are widely distributed intertidal bivalves in China with significant ecological and economic importance, which are sensitive to thermal stress. Our results showed that thermal environments of clam habitats shaped metabolome diversity, which can enhance the resistance of intertidal clams to thermal stress.
Zhi Hu, Xiang‐Tao Wei, Xiaonie Lin, Linxuan Ma, Tianzhe Zhang, Yunwei Dong   +5 more
wiley   +1 more source