Results 61 to 70 of about 1,080 (170)

High‐quality genome of a novel Thermosynechococcaceae species from Namibia and characterization of its protein expression patterns at elevated temperatures

open access: yesMicrobiologyOpen, Volume 13, Issue 5, October 2024.
In this study, the genome of a new thermophilic cyanobacterium, Thermosynechococcaceae cyanobacterium sp. Okahandja, isolated from a hot spring near Okahandja in Namibia, was sequenced. Additionally, cultivations were conducted at elevated temperatures of 40, 50, and 55°C, followed by analyses of the respective adapted proteomes based on the annotated ...
Nathanael D. Arnold   +11 more
wiley   +1 more source

Solubility and availability of VapC influences the growth rate of E. coli. [PDF]

open access: yes, 2014
(A) The growth of E. coli transformed with control pAEØ (◊), pAE-vapB (▪), pAE-vapC (•) or pAE-vapBC (▴) was followed after induction, for 5 h to 8 h. E. coli harboring the empty vector pAE was used as control. The data represents one experiment that was
André L. Sanson (602171)   +8 more
core   +1 more source

Crystal structure of proteolyzed VapBC and DNA‐bound VapBC from Salmonella enterica Typhimurium LT2 and VapC as a putative Ca 2+ ‐dependent ribonuclease [PDF]

open access: yesThe FASEB Journal, 2019
Abstract Bacterial toxin‐antitoxin (TA) system has gained attention for its essential roles in cellular maintenance and survival under harsh environmental conditions such as nutrient deficiency and antibiotic treatment. There are at least 14 TA systems in Salmonella enterica
DongWon, Park   +7 more
openaire   +2 more sources

The Great Codon Escape: Vacating Codons for Genetic Code Expansion and Ribosome Stalling

open access: yesIsrael Journal of Chemistry, Volume 64, Issue 8-9, September 2024.
Abstract In ribosomal synthesis of peptides and proteins, genetic information is translated into an amino acid polymer according to the genetic code, which describes the translational command encoded by each codon. However, parts of the genetic code can be adjusted to customize translations.
Antonius J. P. Hopstaken   +2 more
wiley   +1 more source

Toxin-antitoxin loci vapBC-1 and vapXD contribute to survival and virulence in nontypeable Haemophilus influenzae

open access: yesBMC Microbiology, 2012
Background Nontypeable Haemophilus influenzae (NTHi) is a significant human pathogen responsible for respiratory tract infections and the most common cause of recurrent otitis media. Type II toxin-antitoxin (TA) systems are genetic elements that code for
Ren Dabin, Walker Anna N, Daines Dayle A
doaj   +1 more source

VapC toxins drive cellular dormancy under uranium stress for the extreme thermoacidophile Metallosphaera prunae

open access: yesEnvironmental Microbiology, 2017
Summary When abruptly exposed to toxic levels of hexavalent uranium, the extremely thermoacidophilic archaeon Metallosphaera prunae , originally isolated from an abandoned uranium mine, ceased to grow, and concomitantly exhibited heightened levels of cytosolic ribonuclease ...
Arpan, Mukherjee   +5 more
openaire   +3 more sources

Association of BKV viremia and nephropathy with adverse alloimmune outcomes in kidney transplant recipients

open access: yesClinical Transplantation, Volume 38, Issue 5, May 2024.
Abstract Background Immunosuppression reduction for BK polyoma virus (BKV) must be balanced against risk of adverse alloimmune outcomes. We sought to characterize risk of alloimmune events after BKV within context of HLA‐DR/DQ molecular mismatch (mMM) risk score.
Christie Rampersad   +14 more
wiley   +1 more source

Characterisation of VapC in Mycobacterium smegmatis [PDF]

open access: yes, 2016
Members of the VapC family of proteins cleave RNA at specific sites in order to regulate biological processes with a cell. Characterization of the sites targeted by a specific protein using conventional biochemical techniques is resource intensive.
Willcock, Duncan Mark Koorey
core  

Determination of VapC sequence-specificity using MALDI-TOF MS and urea-denaturing PAGE. [PDF]

open access: yes, 2018
932 RNA Oligo 5 VapC39 MALDI-TOF MS results. RNA Only 0 min and 60 min refer to negative control assay reactions with no VapC protein; 5, 15, 30 and 60 min represent a time course assay with VapC39. Data analysed by Bruker Data Analysis.
Joanna L. Hicks (5706827)   +4 more
core   +1 more source

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