In this study, the genome of a new thermophilic cyanobacterium, Thermosynechococcaceae cyanobacterium sp. Okahandja, isolated from a hot spring near Okahandja in Namibia, was sequenced. Additionally, cultivations were conducted at elevated temperatures of 40, 50, and 55°C, followed by analyses of the respective adapted proteomes based on the annotated ...
Nathanael D. Arnold +11 more
wiley +1 more source
Solubility and availability of VapC influences the growth rate of E. coli. [PDF]
(A) The growth of E. coli transformed with control pAEØ (◊), pAE-vapB (▪), pAE-vapC (•) or pAE-vapBC (▴) was followed after induction, for 5 h to 8 h. E. coli harboring the empty vector pAE was used as control. The data represents one experiment that was
André L. Sanson (602171) +8 more
core +1 more source
Additional file 1 of Bioinformatic and literature assessment of toxicity and allergenicity of a CRISPR-Cas9 engineered gene drive to control Anopheles gambiae the mosquito vector of human malaria [PDF]
Additional file 1. Searches for homologues of F.
John B. Connolly (7674728) +1 more
core +1 more source
Crystal structure of proteolyzed VapBC and DNA‐bound VapBC from Salmonella enterica Typhimurium LT2 and VapC as a putative Ca 2+ ‐dependent ribonuclease [PDF]
Abstract Bacterial toxin‐antitoxin (TA) system has gained attention for its essential roles in cellular maintenance and survival under harsh environmental conditions such as nutrient deficiency and antibiotic treatment. There are at least 14 TA systems in Salmonella enterica
DongWon, Park +7 more
openaire +2 more sources
The Great Codon Escape: Vacating Codons for Genetic Code Expansion and Ribosome Stalling
Abstract In ribosomal synthesis of peptides and proteins, genetic information is translated into an amino acid polymer according to the genetic code, which describes the translational command encoded by each codon. However, parts of the genetic code can be adjusted to customize translations.
Antonius J. P. Hopstaken +2 more
wiley +1 more source
Background Nontypeable Haemophilus influenzae (NTHi) is a significant human pathogen responsible for respiratory tract infections and the most common cause of recurrent otitis media. Type II toxin-antitoxin (TA) systems are genetic elements that code for
Ren Dabin, Walker Anna N, Daines Dayle A
doaj +1 more source
Summary When abruptly exposed to toxic levels of hexavalent uranium, the extremely thermoacidophilic archaeon Metallosphaera prunae , originally isolated from an abandoned uranium mine, ceased to grow, and concomitantly exhibited heightened levels of cytosolic ribonuclease ...
Arpan, Mukherjee +5 more
openaire +3 more sources
Abstract Background Immunosuppression reduction for BK polyoma virus (BKV) must be balanced against risk of adverse alloimmune outcomes. We sought to characterize risk of alloimmune events after BKV within context of HLA‐DR/DQ molecular mismatch (mMM) risk score.
Christie Rampersad +14 more
wiley +1 more source
Characterisation of VapC in Mycobacterium smegmatis [PDF]
Members of the VapC family of proteins cleave RNA at specific sites in order to regulate biological processes with a cell. Characterization of the sites targeted by a specific protein using conventional biochemical techniques is resource intensive.
Willcock, Duncan Mark Koorey
core
Determination of VapC sequence-specificity using MALDI-TOF MS and urea-denaturing PAGE. [PDF]
932 RNA Oligo 5 VapC39 MALDI-TOF MS results. RNA Only 0 min and 60 min refer to negative control assay reactions with no VapC protein; 5, 15, 30 and 60 min represent a time course assay with VapC39. Data analysed by Bruker Data Analysis.
Joanna L. Hicks (5706827) +4 more
core +1 more source

