A VapBC Toxin-Antitoxin Module is a Post-Transcriptional Regulator of Metabolic Flux in Mycobacteria [PDF]
, 2012 Published ahead of print 24 February 2012 The largest family of toxin-antitoxin (TA) modules are encoded by the vapBC operons, but their roles in bacterial physiology remain enigmatic.
Cook, Gregory M. +16 more
core +2 more sources
3D model of leptospiral VapC closely matches the experimental X-ray structure of Shigella's VapC.
, 2014 Alignment of the sequences of VapCs from S. flexneri and L. interrogans is shown. On the left panel, the structure model (ribbon) of VapC from L. interrogans (green) was superimposed to the VapC template from S. flexneri (white) (PdB: 3TND-C).
André L. Sanson (602171) +8 more
core +1 more source
Determination of VapC sequence-specificity using MALDI-TOF MS and urea-denaturing PAGE.
, 2018 932 RNA Oligo 5 VapC39 MALDI-TOF MS results. RNA Only 0 min and 60 min refer to negative control assay reactions with no VapC protein; 5, 15, 30 and 60 min represent a time course assay with VapC39. Data analysed by Bruker Data Analysis.
Joanna L. Hicks (5706827) +4 more
core +1 more source
VapB-1 targets VapC-1 to the vapB-1 translation initiation region.
, 2013 Gel shift products from a titration of VapB-1 into samples following VapC-1 binding to (A) 50TIR or (B) 50US at a 100∶1 molar ratio of protein to DNA.
Sehresh Saleem (329519) +2 more
core +1 more source
Leptospiral VapC toxin cleaves tRNAfMet and is inhibited by the VapB antitoxin.
, 2014 Unless specified, the reactions were carried out under the following general condition: 5 pmol VapC was incubated with 3 ρmol of tRNAfMet in 10 mM Hepes pH 7.5, 15 mM KCl, 1 mM DTT, 10 mM MgCl and 10% glycerol at 37°C for 30 min and analyzed by ...
André L. Sanson (602171) +8 more
core +1 more source
Circular dichroism of VapB and VapC confirmed predicted secondary structure.
, 2014 (A) The VapB and VapC CD spectra were recorded in the wavelength range of 195–255 nm as average of five scans at 20°C. Measured ellipticities, θ (mdegree), were converted to molar mean residue ellipticities, [θ] (degree.cm2.dmol−1).
André L. Sanson (602171) +8 more
core +1 more source
Molecular target of enteric VapC toxins and regulation of vapBC transcription by conditional cooperativity [PDF]
, 2011 The uibiquitous Type II toxin – antitoxin (TA) loci encode two proteins, a toxin and an antitoxin. The antitoxin combines with and neutralizes a cognate toxin. Usually, the TA genes form an operon that is transcribed by a single promoter located upstream
Kristoffer Skovbo Winther +1 more
core
Masking of errors in transmission of VAPC-coded speech [PDF]
, 1990 A subjective evaluation is provided of the bit error sensitivity of the message elements of a Vector Adaptive Predictive (VAPC) speech coder, along with an indication of the amenability of these elements to a popular error masking strategy (cross frame ...
Cox, Neil B., Froese, Edwin L.
core
Determination of ribonuclease sequence-specificity using Pentaprobes and mass spectrometry
, 2012 The VapBC toxin-antitoxin (TA) family is the largest of nine identified TA families. The toxin, VapC, is a metal-dependent ribonuclease that is inhibited by its cognate antitoxin, VapB. Although the VapBCs are the largest TA family, little is known about
Cook, Gregory M. +13 more
core +1 more source
VapC-1 of nontypeable Haemophilus influenzae is a ribonuclease [PDF]
, 2007 Nontypeable Haemophilus influenzae (NTHi) organisms are obligate parasites of the human upper respiratory tract that can exist as commensals or pathogens.
Wu, Mack H +2 more
core