Results 211 to 220 of about 62,350 (249)

Purification of the membrane-form variant surface glycoprotein of Trypanosoma brucei

Journal of Chromatography A, 1990
The membrane-form variant surface glycoprotein (mfVSG) is anchored in the plasma membrane of African trypanosomes by a diacylglycerol residue. On cell rupture the anchor is rapidly cleaved by an endogenous phospholipase C. A purification procedure is described which results in native mfVSG devoid of lipase activity.
D, Schell, P, Overath
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Expression of a minichromosomal variant surface glycoprotein gene in Trypanosoma brucei

Nature, 1985
African trypanosomes contain numerous variant surface glycoprotein (VSG) genes, but express only one at a time. When different VSG genes are activated by gene duplicative or non-duplicative mechanisms, antigenic variation occurs. Although transcriptionally inactive VSG genes can have either an intra-chromosomal or a telomeric location, all expressed ...
V, Rothwell, R, Aline, M, Parsons, N, Agabian, K, Stuart   +4 more
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Endocytosis and intracellular occurrence of the variant surface glycoprotein in Trypanosoma congolense

Journal of Ultrastructure and Molecular Structure Research, 1988
Trypanosoma congolense bloodstream forms were examined for binding sites of polyclonal anti-variant surface glycoprotein (VSG) antibodies using immunoelectron microscopy. Besides the surface, the antibodies labeled intracellular vesicles, the tubular membrane system, secondary lysosomes, and the digestive vacuole. Protein A gold (PAG), peroxidase gold (
U, Frevert, E, Reinwald
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Biochemistry of the Variant Surface Glycoproteins of Salivarian Trypanosomes

1982
Publisher Summary This chapter describes the progress made over the past years in characterizing antigens of the principal pathogenic salivarian trypanosomes. Although bulk of information comes from work on the variant antigens of T. b. brucei in particular, much interesting work has been done on the variant antigens of other species and is discussed
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Changes in cell surface glycoproteins on variant hamster cell lines

International Journal of Biochemistry, 1979
Abstract 1. 1. Resistance to concanavalin A was accompanied by modifications to the fundamental growth and membrane-associated properties of two concanavalin A-resistant cell lines which were independently selected by either single step or cycling procedures. 2. 2.
H, Ceri, J A, Wright
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Variable and conserved structural elements of trypanosome variant surface glycoproteins

Molecular and Biochemical Parasitology, 1992
The characterization of B cell epitopes on the trypanosome variant surface glycoprotein (VSG) rests on elucidation of variant specific amino acid sequences that may be exposed or buried as a result of the natural conformation of these molecules in the surface coat.
D M, Reinitz, B D, Aizenstein, J M, Mansfield   +2 more
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Structure and variationwithin variant surface glycoproteins of Trypanosoma brucei

Annales de l'Institut Pasteur / Immunologie, 1985
Variant surface glycoproteins of the African trypanosomes are members of a multigene family which show extraordinary amino sequence diversity. The extent of this diversity and the significance of homologies both in the amino acid sequence and in the post-translational modifications are discussed in the light of what is predicted for the structure of ...
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Biosynthesis, Attachment and Release of Variant Surface Glycoproteins of the African Trypanosome

1985
Antigenic variation in the African trypanosome is a consequence of changes in the composition of the surface coat which covers the entire surface of the trypanosome, when it is in the blood stream of its mammalian host (reviewed by Vickerman and Barry 1982). The surface coat is made up of a matrix of about 107 identical glycoprotein molecules which are
M J, Turner, M L, Cardoso de Almeida, A M, Gurnett, J, Raper, J, Ward   +4 more
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Trypanosome variant surface glycoprotein genes expressed early in infection

Journal of Molecular Biology, 1985
We have studied further the genes for trypanosomal variant surface glycoproteins expressed during a chronic infection of rabbits with Trypanosoma brucei, strain 427. We show that there are three closely related chromosomal-internal isogenes for VSG 121; expression of one of these genes is accompanied by the duplicate transposition of the gene to a ...
A Y, Liu, P A, Michels, A, Bernards, P, Borst   +3 more
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Rapid evolution of genes coding for variant surface glycoproteins in trypanosomes

Gene, 1982
We have used cloned DNA complementary to the messenger RNAs (mRNAs) for different variant surface glycoproteins (VSGs) of Trypanosoma brucei, stock 427, to study the degree of conservation of the corresponding nuclear genes in related trypanosome stocks.
A C, Frasch, P, Borst, J, Van den Burg
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