Results 41 to 50 of about 1,681,314 (226)

Effect of disinfectants on variola virus in cell culture [PDF]

open access: bronzeApplied and Environmental Microbiology, 1976
Twenty kinds of disinfectants were examined for ability to inactivate variola virus. Cytopathic effect and plaque formation on monolayer cultures of an established monkey kidney cell line were used as indicators of virus inactivation. A micromethod using microplate cultures, and not requiring a CO2 incubator, was adopted.
I Tanabe, S Hotta
openalex   +4 more sources

An Antigenically Atypical Strain of Variola Virus

open access: bronzeJournal of General Virology, 1976
The Kuwait-5-67 strain of variola virus, although indistinguishable from variola virus in its other properties, behaved atypically in immuno-diffusion tests with antivaccinia serum. The precipitation line pattern produced was similar to that of cowpox virus but lacked one of the main precipitation bands given by all other strains of variola, alastrim ...
S. S. Marennikova   +3 more
openalex   +4 more sources

Immunofluorescence staining for detection of variola virus [PDF]

open access: bronzeJournal of Clinical Microbiology, 1981
In September 1975 Bangladesh was the only country in the world with endemic variola major, and the eradication of the disease was imminent. A rapid and accurate laboratory diagnostic method was required to supplement immunodiffusion in agar gel and culture on chorioallantoic membrane of embryonated egg available at the Institute of Public Health in ...
Daniel Tarantola   +3 more
openalex   +3 more sources

Assay of Variola Virus by the Fluorescent Cell-Counting Technique [PDF]

open access: goldApplied Microbiology, 1965
A quantitative assay for infective variola virus particles was developed which is based on the enumeration of cells containing fluorescent viral antigen after infection of McCoy cell monolayers. The direct fluorescent-antibody technique was employed to stain cells.
Nicholas Hahon
  +6 more sources

Variola—a Probable Virus Disease of Citrus [PDF]

open access: bronzeInternational Organization of Citrus Virologists Conference Proceedings (1957-2010), 1965
Ary A. Salibe, Sylvio Moreira
openalex   +4 more sources

Neutralization Determinants on Poxviruses

open access: yesViruses, 2023
Smallpox was a highly contagious disease caused by the variola virus. The disease affected millions of people over thousands of years and variola virus ranked as one of the deadliest viruses in human history. The complete eradication of smallpox in 1980,
Vernuccio Riccardo, Guardado-Calvo Pablo
doaj   +1 more source

Development of a High-Content Orthopoxvirus Infectivity and Neutralization Assays. [PDF]

open access: yesPLoS ONE, 2015
Currently, a number of assays measure Orthopoxvirus neutralization with serum from individuals, vaccinated against smallpox. In addition to the traditional plaque reduction neutralization test (PRNT), newer higher throughput assays are based on ...
Irina Gates   +5 more
doaj   +1 more source

Multi-epitope mRNA Vaccine Design that Exploits Variola Virus and Monkeypox Virus Proteins for Elicitation of Long-lasting Humoral and Cellular Protection Against Severe Disease

open access: yesJournal of Medical Sciences, 2022
Human monkeypox represents a relatively underexplored infection that has received increased attention since the reported outbreak in May 2022. Due to its clinical similarities with human smallpox, this virus represents a potentially tremendous health ...
Dženan Kovačić, Adna Salihović
semanticscholar   +1 more source

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