Quantitative Multiplex Assay for Simultaneous Detection and Identification of Indiana and New Jersey Serotypes of Vesicular Stomatitis Virus [PDF]
Journal of Clinical Microbiology, 2005 ABSTRACT In order to establish a rapid and reliable system for the detection of vesicular stomatitis virus (VSV), we developed a quantitative reverse transcription-PCR assay for the detection, quantification, and differentiation of the major serotypes, VSV Indiana and VSV New Jersey, using a closed-tube multiplex format.
Rasmussen, Thomas B. +3 more
openaire +6 more sources
USE OF REAL-TIME qRT-PCR FOR FMD VIRUS DETECTION IN CATTLE MILK
Ветеринария сегодня, 2019 FMDV can be shed with milk of infected animals and infect susceptible animals. The possibility of using real-time qRT-PCR for FMD virus detection in cattle milk was assessed. To prepare samples of FMDV infected milk the following strains were used: А No.
A. M. Timina +2 more
doaj +1 more source
Single virus fingerprinting by widefield interferometric defocus-enhanced mid-infrared photothermal microscopy [PDF]
arXiv, 2022 Clinical identification and fundamental study of viruses rely on the detection of viral proteins or viral nucleic acids. Yet, amplification-based and antigen-based methods are not able to provide precise compositional information of individual virions due to small particle size and low-abundance chemical contents (e.g., ~ 5000 proteins in a vesicular ...
arxiv
Characterization of Antibody Interactions with the G Protein of Vesicular Stomatitis Virus Indiana Strain and Other Vesiculovirus G Proteins [PDF]
Journal of Virology, 2018 VSVind.G is currently regarded as the gold-standard envelope glycoprotein to pseudotype lentiviral vectors. However, recently other G proteins derived from vesiculoviruses have been proposed as alternative envelopes. Here, we investigated two commercially available anti-VSVind.G monoclonal antibodies for their ability to cross-react with other ...
Mary Collins +8 more
openaire +6 more sources
Predicting essential components of signal transduction networks: a dynamic model of guard cell abscisic acid signaling [PDF]
PLoS Biology 4 (10), e312 (2006), 2006 Plants both lose water and take in carbon dioxide through microscopic stomatal pores, each of which is regulated by a surrounding pair of guard cells. During drought, the plant hormone abscisic acid (ABA) inhibits stomatal opening and promotes stomatal closure, thereby promoting water conservation.
arxiv +1 more source
In-Silico Approaches for Molecular Characterization, Structural Function Prediction and Peptide Toxicity Analysis of the Matrix Protein of Vesicular stomatitis Indiana Virus (VSIV) [PDF]
BIO Web of ConferencesVesicular Stomatitis Indiana Virus (VSIV) is a primary RNA virus studied for its effects in virology and in biotechnology the matrix protein of VSIV is key in linking the viral envelope to the core which is essential role in the assembly and release of ...
S Ramya +3 more
doaj +1 more source
Coronins and their role in immunological phenomena
Central European Journal of Immunology, 2017 Coronins are a large family of proteins occurring in many eukaryotes. In mammals, seven coronin genes have been identified, evidencing that coronins 1 to 6 present classic coronin structure, while coronin 7 is a tandem coronin particle, without a spiral ...
Beata Tokarz-Deptuła +3 more
doaj +1 more source
Human ACE2 Genetic Polymorphism Affecting SARS-CoV and SARS-CoV-2 Entry into Cells
Microbiology Spectrum, 2022 Severe acute respiratory syndrome coronavirus (SARS-CoV) and SARS-CoV-2 have a single envelope glycoprotein (S protein) that binds to human angiotensin-converting enzyme 2 (ACE2) on the host cell membrane.
Takanari Hattori +7 more
doaj +1 more source
Estimating Seroprevalence of SARS-CoV-2 in Ohio: A Bayesian Multilevel Poststratification Approach with Multiple Diagnostic Tests [PDF]
PNAS. 2021;118(26):e2023947118, 2020 Globally the SARS-CoV-2 coronavirus has infected more than 59 million people and killed more than 1.39 million. Designing and monitoring interventions to slow and stop the spread of the virus require knowledge of how many people have been and are currently infected, where they live, and how they interact. The first step is an accurate assessment of the
arxiv +1 more source
Molecular Mechanisms Underlying the Cellular Entry and Host Range Restriction of Lujo Virus
mBio, 2022 Like other human-pathogenic arenaviruses, Lujo virus (LUJV) is a causative agent of viral hemorrhagic fever in humans. LUJV infects humans with high mortality rates, but the susceptibilities of other animal species and the molecular determinants of its ...
Takeshi Saito +6 more
doaj +1 more source