VHH Antibodies: Reagents for Mycotoxin Detection in Food Products [PDF]
Sensors, 2018 Mycotoxins are the toxic secondary metabolites produced by fungi and they are a worldwide public health concern. A VHH antibody (or nanobody) is the smallest antigen binding entity and is produced by heavy chain only antibodies.
Jia Wang +4 more
doaj +3 more sources
Structural Evaluation of EGFR Inhibition Mechanisms for Nanobodies/VHH Domains [PDF]
Structure, 2013 The epidermal growth factor receptor (EGFR) is implicated in human cancers and is the target of several classes of therapeutic agents, including antibody-based drugs. Here, we describe X-ray crystal structures of the extracellular region of EGFR in complex with three inhibitory nanobodies, the variable domains of heavy chain only antibodies (VHH).
Karl R. Schmitz +4 more
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VHH characterization.Recombinant VHHs: Production, characterization and affinity [PDF]
Analytical Biochemistry, 2020 Among the biological approaches to therapeutics, are the cells, such as CAR-T cells engineered or not, the antibodies armed or not, and the smaller protein scaffolds that can be modified to render them specific of other proteins, à la façon of antibodies.
Chabrol, Eric +8 more
openaire +3 more sources
Construction of a Humanized Artificial VHH Library Reproducing Structural Features of Camelid VHHs for Therapeutics [PDF]
Antibodies, 2022 A variable domain of heavy chain antibody (VHH) has different binding properties than conventional antibodies. Conventional antibodies prefer binding to the convex portion of the antigen, whereas VHHs prefer epitopes, such as crevices and clefts on the antigen.
Taihei Murakami +9 more
openaire +3 more sources
VHH-Based Bispecific Antibodies Targeting Cytokine Production [PDF]
Frontiers in Immunology, 2017 Proinflammatory cytokines, such as TNF, IL-6, and IL-1, play pathogenic roles in multiple diseases and are attractive targets for biologic drugs. Because proinflammatory cytokines possess non-redundant protective and immunoregulatory functions, their systemic neutralization carries the potential for unwanted side effects.
М. А. Носенко +7 more
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VHH characterization. Comparison of recombinant with chemically synthesized anti‐HER2 VHH [PDF]
Protein Science, 2019 AbstractIn the continuous exploration of the VHH chemistry, biochemistry and therapeutic future use, we investigated two different production strategies of this small antibody‐like protein, using an anti‐HER2 VHH as a model. The total chemical synthesis of the 125 amino‐acid peptide was performed with reasonable yield, even if optimization will be ...
Lucie Hartmann +14 more
openaire +4 more sources
The Dynamics of Vowel Hypo- and Hyperarticulation in Swedish Infant-Directed Speech to 12-Month-Olds
Frontiers in Communication, 2020 Vowel hypo- and hyperarticulation (VHH) was investigated in Swedish infant-directed speech (IDS) to Swedish 12-month-olds using a measure that normalizes across speakers and vowels: the vhh-index. The vhh-index gives the degree of VHH for each individual
Ellen Marklund, Lisa Gustavsson
doaj +1 more source
Distinct types of VHHs in Alpaca [PDF]
Frontiers in ImmunologyIntroductionVHHs (VH of heavy-chain-only antibodies) represent a unique alternative to Q7 conventional antibodies because of their smaller size, comparable binding affinity and biophysical properties. MethodIn this study, we systematically analyzed VHH NGS sequences from 22 Alpacas and structure data from public database.
Xinhao Wang +5 more
openalex +4 more sources
A gp41 MPER-specific llama VHH requires a hydrophobic CDR3 for neutralization but not for antigen recognition [PDF]
, 2013 The membrane proximal external region (MPER) of the HIV-1 glycoprotein gp41 is targeted by the broadly neutralizing antibodies 2F5 and 4E10. To date, no immunization regimen in animals or humans has produced HIV-1 neutralizing MPER-specific antibodies ...
Aasa-Chapman, Marlén M. I. +23 more
core +10 more sources
Seed-produced anti-globulin VHH-Fc antibodies retrieve globulin precursors in the insoluble fraction and modulate the Arabidopsis thaliana seed subcellular morphology [PDF]
, 2020 Key message Nanobody-heavy chain (VHH-Fc) antibody formats have the potential to immunomodulate even highly accumulating proteins and provide a valuable tool to experimentally modulate the subcellular distribution of seed storage proteins.
Altmann, Friedrich +7 more
core +2 more sources