Results 1 to 10 of about 421,919 (120)

Development of a Cell-Based Luciferase Complementation Assay for Identification of SARS-CoV-2 3CL^pro Inhibitors

Viruses, 2021
The 3C-like protease (3CLpro) of SARS-CoV-2 is considered an excellent target for COVID-19 antiviral drug development because it is essential for viral replication and has a cleavage specificity distinct from human proteases.
Jonathan M. O. Rawson, Alice Duchon, Olga A. Nikolaitchik, Vinay K. Pathak, Wei-Shau Hu   +4 more
doaj   +1 more source

Plasma Membrane Anchoring and Gag:Gag Multimerization on Viral RNA Are Critical Properties of HIV-1 Gag Required To Mediate Efficient Genome Packaging

mBio, 2021
Human immunodeficiency virus type 1 (HIV-1) Gag selects and packages the HIV RNA genome during virus assembly. However, HIV-1 RNA constitutes only a small fraction of the cellular RNA.
Alice Duchon, Steven Santos, Jianbo Chen, Matthew Brown, Olga A. Nikolaitchik, Sheldon Tai, Jeffrey A. Chao, Eric O. Freed, Vinay K. Pathak, Wei-Shau Hu   +9 more
doaj   +1 more source

Adaptation of HIV-1/HIV-2 Chimeras with Defects in Genome Packaging and Viral Replication

mBio, 2022
Frequent recombination is a hallmark of retrovirus replication. In rare cases, recombination occurs between distantly related retroviruses, generating novel viruses that may significantly impact viral evolution and public health.
Jonathan M. O. Rawson, Olga A. Nikolaitchik, Jennifer A. Yoo, Xayathed Somoulay, Matthew A. Brown, Franck S. Mbuntcha Bogni, Vinay K. Pathak, Ferri Soheilian, Ryan L. Slack, Stefan G. Sarafianos, Wei-Shau Hu   +10 more
doaj   +1 more source

Transcription Start Site Heterogeneity and Preferential Packaging of Specific Full-Length RNA Species Are Conserved Features of Primate Lentiviruses

Microbiology Spectrum, 2022
HIV-1 must package its RNA genome to generate infectious viruses. Recent studies have revealed that during genome packaging, HIV-1 not only excludes cellular mRNAs, but also distinguishes among full-length viral RNAs. Using NL4-3 and MAL molecular clones,
Jonathan M. O. Rawson, Olga A. Nikolaitchik, Saurabh Shakya, Brandon F. Keele, Vinay K. Pathak, Wei-Shau Hu   +5 more
doaj   +1 more source

Potent dual block to HIV-1 infection using lentiviral vectors expressing fusion inhibitor peptide mC46- and Vif-resistant APOBEC3G

Molecular Therapy: Nucleic Acids, 2023
Gene therapy strategies that effectively inhibit HIV-1 replication are needed to reduce the requirement for lifelong antiviral therapy and potentially achieve a functional cure.
Krista A. Delviks-Frankenberry, Chet R. Ojha, Kip J. Hermann, Wei-Shau Hu, Bruce E. Torbett, Vinay K. Pathak   +5 more
doaj   +1 more source

Impact of Nuclear Export Pathway on Cytoplasmic HIV-1 RNA Transport Mechanism and Distribution

mBio, 2020
HIV-1 full-length RNA (referred to as HIV-1 RNA here) serves as the viral genome in virions and as a template for Gag/Gag-Pol translation. We previously showed that HIV-1 RNA, which is exported via the CRM1 pathway, travels in the cytoplasm mainly ...
Jianbo Chen, Chijioke Umunnakwe, David Q. Sun, Olga A. Nikolaitchik, Vinay K. Pathak, Ben Berkhout, Atze T. Das, Wei-Shau Hu   +7 more
doaj   +1 more source

Chinese Giant Salamander Iridovirus 025L Is a Viral Essential Gene

Viruses, 2023
Ranavirus is a large nucleocytoplasmic DNA virus. Chinese giant salamander iridovirus (CGSIV) belongs to the ranavirus genus, and its replication involves a series of essential viral genes.
Zijing Liu, Daofa Xie, Shirong Nong, Yingzi Wu, Suxian Huang, Xianhui He, Tianhong Zhou, Wei Li   +7 more
doaj   +1 more source

Structural Insights into APOBEC3-Mediated Lentiviral Restriction

Viruses, 2020
Mammals have developed clever adaptive and innate immune defense mechanisms to protect against invading bacterial and viral pathogens. Human innate immunity is continuously evolving to expand the repertoire of restriction factors and one such family of ...
Krista A. Delviks-Frankenberry, Belete A. Desimmie, Vinay K. Pathak   +2 more
doaj   +1 more source

Hepatitis C Viral Replication Complex

Viruses, 2021
The life cycle of the hepatitis C virus (HCV) can be divided into several stages, including viral entry, protein translation, RNA replication, viral assembly, and release.
Hui-Chun Li, Chee-Hing Yang, Shih-Yen Lo
doaj   +1 more source

Secretory Carrier Membrane Protein 3 Interacts with 3A Viral Protein of Enterovirus and Participates in Viral Replication

Microbiology Spectrum, 2021
Picornaviruses are a diverse and major cause of human disease, and their genomes replicate with intracellular membranes. The functionality of these replication organelles depends on the activities of both viral nonstructural proteins and co-opted host ...
Jia-Ying Lu, Gary Brewer, Mei-Ling Li, Kai-Zhe Lin, Chien-Chih Huang, Li-Chen Yen, Jing-Yi Lin   +6 more
doaj   +1 more source