Results 71 to 80 of about 7,059,643 (153)

Molecular genetic analysis of siRNA biogenesis and function in Arabidopsis thaliana [PDF]

, 2009
In diverse eukaryotes, small RNA products of Dicer-like (DCL) proteins regulate mRNA stability or translation, and direct chromatin modifications to genomic regions, phenomena collectively known as RNA silencing.
Blevins, Todd Lucas
core   +1 more source

Protocol for triggering plant gene silencing by viral delivery of short RNA

STAR Protocols
Summary: Virus-induced gene silencing (VIGS) leverages viral vectors to deliver 200–400-nt inserts targeting specific genes. Here, we present a protocol for triggering gene silencing by virus-delivered short RNA inserts (vsRNAi).
Arcadio García, Verónica Aragonés, Paloma Ortiz-García, José-Antonio Daròs, Fabio Pasin   +4 more
doaj   +1 more source

Exploring plant genomes by RNA-induced gene silencing

, 2003
The nucleotide sequences of several animal, plant and bacterial genomes are now known, but the functions of many of the proteins that they are predicted to encode remain unclear.
Helliwell, C. A., Peter M. Waterhouse, Christopher A. Helliwell, Waterhouse, P. M.   +3 more
core   +1 more source

Induction and maintenance of DNA methylation in plant promoter sequences by apple latent spherical virus-induced transcriptional gene silencing

Frontiers in Microbiology, 2014
Apple latent spherical virus (ALSV) is an efficient virus-induced gene silencing vector in functional genomics analyses of a broad range of plant species.
Tatsuya eKon, Nobuyuki eYoshikawa
doaj   +1 more source

Knocking down wheat meiotic genes through Virus-induced gene-silencing (VIGS)

, 2019
Virus-induced gene-silencing (VIGS) is a reverse genetic technology that can be used to assess gene function in model and crop species. The technique exploits a natural viral defence response in plants, whereby invading viral RNAs are detected, and this ...
Kanyuka, K., Higgins, J. D., Desjardins, S.   +2 more
core  

A Foxtail mosaic virus Vector for Virus-Induced Gene Silencing in Maize [PDF]

, 2016
Plant viruses have been widely used as vectors for foreign gene expression and virus-induced gene silencing (VIGS). A limited number of viruses have been developed into viral vectors for the purposes of gene expression or VIGS in monocotyledonous plants,
Kernodle, Bliss, Whitham, Steven, Mei, Yu, Hill, John, Zhang, Chunquan, Hill, John H, Whitham, Steven A, Kernodle, Bliss M   +7 more
core   +1 more source

Evaluation of Tobacco Rattle Virus-based Vectors for Gene Silencing and Editing in Tomato

Journal of the American Society for Horticultural Science
This study established an optimized protocol for tobacco rattle virus (TRV)-based virus-induced gene silencing (VIGS) and virus-induced gene editing (VIGE) in tomato using phytoene desaturase (PDS) as a visual marker.
Hsin-Mei Ku, Jui-Chieh Liu, Shang-Ling Ou, Hui-Ju Chang, Sheng-Wen Wang, Ping-Yen Chiu, Guan-Ling Chen, Chien-Chi Huang, Chien-Hung Chung   +8 more
doaj   +1 more source

RNA duplexes in transcriptional regulation

Biomolecular Concepts, 2010
Transcriptional regulation by small RNA molecules, including small interfering RNA and microRNA, has emerged as an important gene expression modulator.
Swaminathan Sanjay, Hood Chantelle L., Suzuki Kazuo, Kelleher Anthony D.   +3 more
doaj   +1 more source

Argonaute 2 inhibits RIG-I signaling via competition for viral RNA binding

iScience
Summary: Interferon (IFN)-dependent responses constitute a critical initial defense against viruses in mammalian cells, while RNA interference (RNAi) acts as an additional strategy to combat invading viral pathogens.
Honglian Liu, Yingyin Liao, Fei Yu, Leo Ngo-Shing Li, Yajie Zhang, Lin Zhu, Guangshan Xie, Jiayan Liu, Siwen Liu, Shaofeng Deng, Rachel Chun-Yee Tam, Wenjun Song, Pin Chen, Xiaofeng Huang, Conor J. Cremin, Yixin Chen, Min Zheng, Pui Wang, Zongwei Cai, Kwok-Yung Yuen, Honglin Chen, Bobo Wing-Yee Mok   +21 more
doaj   +1 more source

Virus-induced gene silencing (VIGS) in Cannabis sativa L.

Plant Methods, 2019
Background The raised demand of cannabis as a medicinal plant in recent years led to an increased interest in understanding the biosynthetic routes of cannabis metabolites.
Julia Schachtsiek, Tajammul Hussain, Khadija Azzouhri, Oliver Kayser, Felix Stehle   +4 more
doaj   +1 more source