Results 51 to 60 of about 7,059,643 (153)

P25 and P37 proteins encoded by firespike leafroll-associated virus are viral suppressors of RNA silencing

Frontiers in Microbiology, 2022
Firespike leafroll-associated virus (FLRaV) is a major pathogen associated with firespike (Odontonema tubaeforme) leafroll disease. Phylogenetic analysis showed that FLRaV possesses typical traits of subgroup II members of ampeloviruses, but encodes two ...
Yaqin Wang, Hui Liu, Zhanqi Wang, Yushuang Guo, Tao Hu, Xueping Zhou, Xueping Zhou   +6 more
doaj   +1 more source

Virus induced gene silencing (VIGS) for functional anaylsis of wheat genes involved in Zymoseptoria tritici susceptibiblity and resistance [PDF]

, 2015
Virus-induced gene silencing (VIGS) has emerged as a powerful reverse genetic technology in plants supplementary to stable transgenic RNAi and, in certain species, as a viable alternative approach for gene functional analysis. The RNA virus Barley stripe
Rudd, J. J., Kanyuka, K., Lee, W-S., Kanyuka, Kostya, Rudd, Jason   +4 more
core   +1 more source

VIGS as a strategy to reverse aphid wing induction by Y‐satellite RNA of cucumber mosaic virus

FEBS Open Bio, 2023
Y‐satellite RNA (Y‐sat) of cucumber mosaic virus upregulates the expression of the aphid ABCG4 gene, which promotes aphid wing formation. We used ABCG4 virus‐induced gene silencing (VIGS) to prevent the wing‐induction mechanism of Y‐sat and thus ...
Hangil Kim, Chikara Masuta
doaj   +1 more source

The NS3 protein of rice hoja blanca virus suppresses RNA silencing in mammalian cells [PDF]

, 2008
The NS3 protein of the tenuivirus rice hoja blanca virus (RHBV) has previously been shown to represent the viral RNA interference (RNAi) suppressor and is active in both plant and insect cells by binding short interfering RNAs (siRNAs) in vitro.
Hemmes, J.C., Schnettler, E., Goldbach, R.W., Prins, M.W.   +3 more
core   +1 more source

Rapid Construction and Application of a Vector for Tobacco Ringspot Virus-Induced McPDS Silencing in Bitter Gourd

Horticulturae
The aim of this study is to facilitate the construction of virus-induced gene silencing vectors and to provide a reference or positive control for gene silencing in bitter gourd.
Lingen Zeng, Hui Zhang, Jinju Guo, Zhijun Deng, Hongbiao Liu, Hu Du, Yujuan Zhong, Changyuan Zhang, Chunpeng Yao   +8 more
doaj   +1 more source

Cassava geminivirus agroclones for virus-induced gene silencing in cassava leaves and roots

Plant Methods, 2018
Aim We report the construction of a Virus-Induced Gene Silencing (VIGS) vector and an agroinoculation protocol for gene silencing in cassava (Manihot esculenta Crantz) leaves and roots.
Ezequiel Matias Lentz, Joel-Elias Kuon, Adrian Alder, Nathalie Mangel, Ima M. Zainuddin, Emily Jane McCallum, Ravi Bodampalli Anjanappa, Wilhelm Gruissem, Hervé Vanderschuren   +8 more
doaj   +1 more source

Modification of Tobacco rattle virus RNA1 to Serve as a VIGS Vector Reveals That the 29K Movement Protein Is an RNA Silencing Suppressor of the Virus

Molecular Plant-Microbe Interactions, 2013
Tobacco rattle virus (TRV) has a bipartite, positive-sense single-stranded RNA genome and is widely used for virus-induced gene silencing (VIGS) in plants.
Xianbao Deng, Jani Kelloniemi, Tuuli Haikonen, Anssi L. Vuorinen, Paula Elomaa, Teemu H. Teeri, Jari P. T. Valkonen   +6 more
doaj   +1 more source

Establishment of a TRV-VIGS system in Coix (Coix lacryma-jobi L.) for functional study of host–pathogen interactions

Frontiers in Plant Science
Coix (Coix lacryma-jobi L.) is an important cereal and medicinal crop threatened by smut disease (Ustilago coicis). However, the unavailability of rapid and efficient functional genomics tools has impeded progress in understanding gene function and host ...
Xiaosheng Zhao, Guocui Wang, Fang He, Xiang-Dong Li, Chunli Zhou, Qiuping Liu, Leitao Tan, Qingbei Weng, Qingbei Weng   +8 more
doaj   +1 more source

Next generation sequencing for studying viruses and RNA silencing-based antiviral defense in crop plants [PDF]

, 2014
The main objectives of this work have been to use next generation sequencing (NGS) and develop bioinformatics tools for plant virus diagnostics and genome reconstruction as well as for investigation of RNA silencing-based antiviral defense.
Seguin, Jonathan
core   +1 more source

RNA silencing is activated by N gene-mediated hypersensitive response and plays a key role in local and systemic virus resistance

Phytopathology Research
In plants, recognition between resistance gene (R) and virus induces a local hypersensitive response (HR), which is accompanied by systemic acquired resistance (SAR).
Wenjing Li, Ru Yu, Wenjuan Liu, Hong Zhang, Chengyang Ren, Yan Zhang, Haoran Ma, Changxiang Zhu, Hongmei Liu   +8 more
doaj   +1 more source