Results 41 to 50 of about 7,059,643 (153)

Diverging affinity of tospovirus RNA silencing suppressor proteins, NSs, for various RNA duplex molecules [PDF]

, 2010
The tospovirus NSs protein was previously shown to suppress the antiviral RNA silencing mechanism in plants. Here the biochemical analysis of NSs proteins from different tospoviruses, using purified NSs or NSs containing cell extracts, is described.
Kormelink, R.J.M., Rik Huismann, Esther Schnettler, Rob Goldbach, Goldbach, R., Marcel Prins, Goldbach, R.W., Kormelink, R., Hemmes, H., Prins, M., Huismann, R., Hans Hemmes, Huisman, R., Hemmes, J.C., Schnettler, E., Richard Kormelink, Prins, M.W.   +16 more
core   +1 more source

Involvement of cathepsin B in the plant disease resistance hypersensitive response [PDF]

, 2007
A diverse range of plant proteases are implicated in pathogen perception and in subsequent signalling and execution of disease resistance. We demonstrate, using protease inhibitors and virus-induced gene silencing (VIGS), that the plant papain cysteine ...
Shaw, Jane, Hein, I., Christophe Lacomme, Kaffarnik, Florian, Hein, Ingo, Lopez, Eduardo C., Eduard Venter, Van Der Hoorn, Renier, Paul R. J. Birch, Pritchard, L., Borras-Hidalgo, Orlando, Hazel McLellan, Gilroy, Eleanor M., McLellan, H., Eleanor M. Gilroy, Hrubikova, Katarina, Birch, P., Orlando Borras-Hidalgo, Boevink, P., Eduardo C. López, Shaw, J., Maria Holeva, Jane Shaw, Loake, Gary J., Hrubikova, K., Katarina Hrubikova, Boevink, Petra C., Holeva, Maria, Venter, Eduard, Kaffarnik, F., McLellan, Hazel, Van der Hoorn, R., Venter, E., Lopez, E., Gary J. Loake, Hein, Ingo; id_orcid, Florian Kaffarnik, Renier Van Der Hoorn, Birch, Paul R. J.; id_orcid, Lacomme, Christophe, Loake, G., Borras-Hidalgo, O., Pritchard, Leighton, Lacomme, C., Ingo Hein, Birch, Paul R. J., Leighton Pritchard, Holeva, M., Gilroy, E., Petra C. Boevink   +49 more
core   +1 more source

Virus-induced Gene Silencing (VIGS) in Phalaenopsis Orchids

Bio-Protocol, 2014
This is a protocol to produce stable silencing efficacy and efficiency for VIGS using CymMV as a silencing vector for floral functional genomics in Phalaenopsis orchids. This protocol is established based on a method created by Lu et al. (2007), and then
Ming-Hsien Hsieh, Zhao-Jun Pan, Hsin-Hung Yeh, Hong-Hwa Chen   +3 more
doaj   +1 more source

Influence of viral genes on the cell-to-cell spread of RNA silencing [PDF]

The turnip crinkle virus-based vector TCV–GFPDCP had been devised previously to study cell-to-cell and long-distance spread of virus-induced RNA silencing.
Hong, Yiguo, Zhou, Yu, Ryabov, Eugene V., Zhang, Xuemei   +3 more
core   +1 more source

Virus-Mediated System for Simultaneous Gene Silencing and Genome Editing in Cotton

Plants
Plant viral vectors are powerful tools for the transient expression of exogenous genes, enabling not only virus-induced gene silencing (VIGS) but also virus-induced genome editing (VIGE).
Yufeng Zheng, Lianjia Zhao, Yulin Tian, Jiahao Lin, Xiaodong Liu, Jianfeng Lei   +5 more
doaj   +1 more source

The role of TNRC6 proteins in gene silencing [PDF]

, 2013
Proteins of the GW182 family have recently emerged as key players in miRNAmediated gene silencing. They have been shown to interact with Argonaute proteins, components of the RISC and are assumed to mediate the repression in metazoa. Three paralogues are
Zipprich, Jakob Theophil
core   +1 more source

Simultaneous gene expression and multi-gene silencing in Zea mays using maize dwarf mosaic virus

BMC Plant Biology, 2021
Background Maize dwarf mosaic virus (MDMV), a member of the genus Potyvirus, infects maize and is non-persistently transmitted by aphids. Several plant viruses have been developed as tools for gene expression and gene silencing in plants. The capacity of
Wenshuang Xie, Dee Marie Marty, Junhuan Xu, Nitika Khatri, Kristen Willie, Wanderson Bucker Moraes, Lucy R. Stewart   +6 more
doaj   +1 more source

Virus induced gene silencing, a post transcriptional gene silencing method [PDF]

, 2009
Virus-induced gene silencing (VIGS) is one of the reverse genetics tools for analysis of gene function that uses viral vectors carrying a target gene fragment to produce dsRNA which trigger RNA-mediated gene silencing. There are a number of viruses which
Unver, Turgay, Budak, Hikmet
core   +1 more source

Inoculation of Maize with Sugarcane Mosaic Virus Constructs and Application for RNA Interference in Fall Armyworms

Bio-Protocol, 2023
Virus-mediated transient gene overexpression and gene expression silencing can be used to screen gene functions in plants. Sugarcane mosaic virus (SCMV) is a positive strand RNA virus in the Potyviridae family that has been modified to be used as vector ...
Iram Gull, Georg Jander
doaj   +1 more source

Virus-induced Gene Silencing (VIGS) in Barley Seedling Leaves

Bio-Protocol, 2015
Virus induced gene silencing (VIGS) is one of the most potent reverse genetics technologies for gene functional characterisation. This method exploits a dsRNA-mediated antiviral defence mechanism in plants.
Lokanadha Gunupuru, Shahin Ali, Fiona Doohan, Steven Scofield   +3 more
doaj   +1 more source