Molecular Plant-Microbe Interactions, 2003 The ChlH gene, encoding for the H subunit of the magnesium chelatase enzyme, was silenced in Nicotiana bentahamiana plants by virus-induced gene silencing (VIGS), using tobacco mosaic virus (TMV) expression vector.
Jean-Baptiste Hiriart +2 more
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Influence of retinoblastoma-related gene silencing on the initiation of DNA replication by African cassava mosaic virus Rep in cells of mature leaves in Nicotiana benthamiana plants [PDF]
, 2011 Background Geminiviruses mainly infect terminally differentiated tissues and cells in plants. They need to reprogramme host cellular machinery for DNA replication.
Yule Liu +14 more
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Virus‐induced gene silencing database for phenomics and functional genomics in Nicotiana benthamiana
Plant Direct, 2018 Virus‐induced gene silencing (VIGS) is an important forward and reverse genetics method for the study of gene function in many plant species, especially Nicotiana benthamiana.
Muthappa Senthil‐Kumar +15 more
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Chinese Wheat Mosaic Virus-Induced Gene Silencing in Monocots and Dicots at Low Temperature
Frontiers in Plant Science, 2018 Virus-induced gene silencing (VIGS) is an important tool for functional genomics studies in plants. With this method, it is possible to target most endogenous genes and downregulate the messenger RNA (mRNA) in a sequence-specific manner.
Jian Yang +13 more
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Legume Science, 2023 Virus‐induced gene silencing (VIGS) is a functional genomics tool used to determine the function of unknown genes or assess the impact of gene silencing on plant phenotype.
Ruyi Xiong +3 more
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Application and Expansion of Virus-Induced Gene Silencing for Functional Studies in Vegetables
Horticulturae, 2023 Increased consumption of vegetables has been recommended worldwide as a part of a healthy diet; therefore, determining gene function among breeding materials is crucial for vegetable improvement to meet the sustainable development of new vegetable ...
Zheng Wang +7 more
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Virus-Induced Gene Silencing of Cell Wall Genes in Flax (Linum usitatissimum)
, 2020 International audiencePlants have developed defense mechanisms against viruses by using an RNA silencing-based process, which has many common features with the endogenous RNA silencing pathway used for regulating the level of transcripts derived from ...
Godfrey Neutelings +5 more
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Defense pathways in Arabidopsis restricting compatible tobamoviruses during infection [PDF]
, 2014 Virus infected plants often develop strong disease symptoms including leaf deformation, chlorosis, necrosis and growth inhibition. In agriculture systems virus infection can lead to severe yield losses and a better understanding of plant defenses against
Kørner, Camilla Julie
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Catch Me If You Can! RNA Silencing-Based Improvement of Antiviral Plant Immunity
Viruses, 2019 Viruses are obligate parasites which cause a range of severe plant diseases that affect farm productivity around the world, resulting in immense annual losses of yield.
Fatima Yousif Gaffar, Aline Koch
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A Cytological Analysis of Wheat Meiosis Targeted by Virus-Induced Gene Silencing (VIGS) [PDF]
, 2019 Virus-induced gene silencing (VIGS) is a rapid and cost-effective reverse genetic technology that can be used to assess gene function in wheat. This chapter contains a detailed description of how to target wheat meiotic genes by VIGS.
Kanyuka, K. +6 more
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