Results 61 to 70 of about 26,425 (258)

Engineered Transformer Base Editor with Enhanced Editing Efficiency

Advanced Science, EarlyView.
A highly efficient transformer base editor system achieves robust genomic editing in a humanized mouse model. This work establishes a versatile and translatable platform, opening new avenues for precision gene therapy. ABSTRACT Canonical cytosine base editors (CBEs) achieve precise C‐to‐T conversions without inducing DNA double‐strand breaks (DSBs ...
Bowen Chen, Letong Liang, Rui Xu, Tianyi Wang, Hongyan Chen, Daru Lu   +5 more
wiley   +1 more source

Virus-like particle vaccines containing F or F and G proteins confer protection against respiratory syncytial virus without pulmonary inflammation in cotton rats

Human Vaccines & Immunotherapeutics, 2017
Vaccine-enhanced disease has been a major obstacle in developing a safe vaccine against respiratory syncytial virus (RSV). This study demonstrates the immunogenicity, efficacy, and safety of virus-like particle (VLP) vaccines containing RSV F (F VLP), G (
Hye Suk Hwang, Ki-Hye Kim, Youri Lee, Young-Tae Lee, Eun-Ju Ko, SooJin Park, Jong Seok Lee, Byung-cheol Lee, Young-Man Kwon, Martin L. Moore, Sang-Moo Kang   +10 more
doaj   +1 more source

Expression of dengue virus and Zika virus NS2B-NS3pro constructs alter cellular fatty acids, but co-expression with a Zika virus virus-like particle is detrimental to virus-like particle expression

BMC Research Notes, 2023
Objective Studies have shown that Flavivirus infection remodels the host cell to favour viral replication. In particular, the host cell lipid profile is altered, and it has been proposed that this process alters membrane fluidity to allow wrapping of the
Suwipa Ramphan, Nathamon Yimpring, Chontida Tangsongcharoen, Suthatta Sornprasert, Atitaya Hitakarun, Wannapa Sornjai, Sittiruk Roytrakul, Atikorn Panya, Duncan R. Smith   +8 more
doaj   +1 more source

Molecular pharming — VLPs made in plants

Current Opinion in Biotechnology, 2016
Plant-based expression offers a safe, inexpensive and potentially limitless way to produce therapeutics in a quick and flexible manner. Plants require only simple inorganic nutrients, water, carbon dioxide and sunlight for efficient growth. Virus-like particles (VLPs) are convincing look-alikes of viruses but without carrying infectious genomic ...
Marsian, Johanna, Lomonossoff, George P
openaire   +2 more sources

Hormonal control and signaling of the testis of the bat Molossus molossus during the processes of testicular maturation, regression, and recrudescence

The Anatomical Record, EarlyView.
Abstract Molossus molossus, a species of neotropical bat belonging to the Molossidae family, has an annual reproductive cycle that includes two peaks of spermatogenic activity, one in April and another in September, and a period of total testicular regression in December.
Emília M. Soares, Juliana F. Ferraz, Cornélio S. Santiago, Larissa P. R. Venâncio, Arlindo G. Macedo‐Junior, Sebastião R. Taboga, Mateus R. Beguelini   +6 more
wiley   +1 more source

Establishment of a yeast-based VLP platform for antigen presentation

Microbial Cell Factories, 2018
Background Chimeric virus-like particles (VLP) allow the display of foreign antigens on their surface and have proved valuable in the development of safe subunit vaccines or drug delivery.
David Wetzel, Theresa Rolf, Manfred Suckow, Andreas Kranz, Andreas Barbian, Jo-Anne Chan, Joachim Leitsch, Michael Weniger, Volker Jenzelewski, Betty Kouskousis, Catherine Palmer, James G. Beeson, Gerhard Schembecker, Juliane Merz, Michael Piontek   +14 more
doaj   +1 more source

Engineering Biology Beyond Single Genes: Advances and Challenges in Multiplex Genome Editing

Animal Research and One Health, EarlyView.
Multiplex genome editing is transforming genome engineering from single‐gene perturbation to network‐level control, yet its broader application remains limited by challenges in gRNA array engineering, delivery technologies, and safety management. Emerging AI‐driven approaches are accelerating guide RNA design and CRISPR effector optimization for ...
Linli Wang, Yongbin Liu, Hongbing Han
wiley   +1 more source

Mesothelin virus-like particle immunization controls pancreatic cancer growth through CD8+ T cell induction and reduction in the frequency of CD4+ foxp3+ ICOS- regulatory T cells. [PDF]

PLoS ONE, 2013
Our previous study has shown that mesothelin (MSLN) is a potential immunotherapeutic target for pancreatic cancer. Here, we further studied the immunogenicity of chimeric murine MSLN-virus-like particles (mMSLN-VLPs), their ability to break tolerance to ...
Sheng Zhang, Lin-Kin Yong, Dali Li, Rafael Cubas, Changyi Chen, Qizhi Yao   +5 more
doaj   +1 more source

ACUTE MYELOID LEUKEMIA IN COMPLETE RESPONSE FOR 2 YEARS WITH AZACYTIDINE ASSOCIATED WITH HIGH-DOSE VITAMINS

Hematology, Transfusion and Cell Therapy, 2023
Acute Myeloid Leukemia (AML) is an uncommon neoplasia, and its treatment is challenging. The AZAVIT-ABCEF protocol (Plataforma Brasil-CAAE: 53015421.0.0000.5463) is of low intensity and reasonably well-tolerated. Objective: To report a clinical case of a
VM Sthel, VLP Figueiredo
doaj   +1 more source

Second‐Generation Polyhydroxybutyrate Production From Pretreated Corn Lignocellulosic Fraction as a Strategy to Repurpose by Products From the Corn‐Processing Chain

Biotechnology and Applied Biochemistry, EarlyView.
ABSTRACT Lignocellulosic residues are non‐cost, non‐edible, renewable feedstocks that can be used in polyhydroxybutyrate (PHB) production. PHB is a bioplastic synthetized by microorganisms, formed intracellularly under stressful growth conditions; however, its production costs are high, representing the major drawback for fossil‐based plastics ...
Clara Matte Borges Machado, Luciana Porto de Souza Vandenberghe, Ariane Fátima Murawski de Mello, Gustavo Amaro Bittencourt, Alexandra M. Rozhkova, Ivan N. Zorov, Susan Grace Karp, Vittoria Gomes Michelin, Carlos Ricardo Soccol   +8 more
wiley   +1 more source