Results 101 to 110 of about 39,685 (269)
Deletion in the agnogene prevents VP1 expression.
, 2013 Western blots for VP1 were done as described in Figure 2. (A) VP1 levels in Cos-7 cell lysate 14 days post-transfection. (B) VP1 levels in Cos-7 cells 21 days post-transfection.
Laura C. Ellis (483454) +3 more
core +1 more source
Biotechnology Reports, 2015 The icosahedral capsid structure of simian virus 40 (diameter, 45 nm) consists of 72 pentameric subunits, with each subunit formed by five VP1 molecules. Electron microscopy, immuno-gold labeling, and ζ-potential analysis showed that purified recombinant
Masaaki Kawano +10 more
doaj +1 more source
Journal of General Virology, 2001 Murine polyomavirus VP1 virus-like particles (VLPs) can bind plasmid DNA and transport it into cells both in vitro and in vivo. Long-term expression of the transgene can be observed, suggesting that VP1 VLPs may be used as DNA delivery vehicles for gene therapy.
B, Clark +4 more
openaire +2 more sources
Nuclear localization of MCV VP2 expressed +/− VP1.
, 2013 Confocal immunofluorescent microscopy of 293TT cells transfected with VP1 (red), VP2 (green) or VP1 and VP2 from a bicistronic plasmid. A mouse monoclonal antibody was used to detect VP1, and a rabbit polyclonal antibody was used to detect VP2 ...
Christopher B. Buck (121726) +1 more
core +1 more source
A Mechanistic Model of rAAV Production in Synthetic Cell Lines
Biotechnology and Bioengineering, Volume 123, Issue 6, Page 1684-1694, June 2026.ABSTRACT The recombinant adeno‐associated virus (rAAV) is a widely used vector for gene therapy. Its manufacturing faces significant challenges in producing the large quantities of vectors needed for clinical applications and reducing empty particles.
Prahalad Srinivasan +5 more
wiley +1 more source
, 2012 Side by side comparison of the VP1 structures from the native virion (A) and the 80S particle (B), showing the hinge movement displacing the αA helix and the C-terminus of VP1 away from the VP1 β-barrel. The secondary structural elements are indicated on
Damià Garriga (189714) +6 more
core +1 more source
The Effect of Protein Tagging on Aggregation and Phase Separation
Journal of Cellular Biochemistry, Volume 127, Issue 6, June 2026.ABSTRACT Protein tags are widely used for purification, solubilization, detection, and imaging, yet they can substantially alter protein self‐assembly. This interference is particularly significant for intrinsically disordered proteins and low‐complexity domains, whose aggregation and phase separation are mediated by weak multivalent interactions that ...
Harunobu Saito, Kenji Sugase
wiley +1 more source
Mapping of differences between LPyV and HPyV9 VP1.
, 2013 The LPyV VP1 structure is shown in surface representation viewed from the side of VP1 (A), from the top of VP1 (B) and from the bottom of VP1 (C). The N-terminal arms were omitted for clarity.
Ten Feizi (114959) +7 more
core +1 more source
Applying Distinct CDMS Strategies to Observe Nonclassical Virus Capsid Assembly
Journal of Mass Spectrometry, Volume 61, Issue 6, June 2026.ABSTRACT In conventional native mass spectrometry (MS), one faces severe limitations when challenged with heterogeneous, high‐mass samples, commonly failing to resolve clear peak distributions, and thus mass determination. Charge detection MS (CDMS) has emerged as a premier method to analyze these samples by determining mass‐to‐charge ratio (m/z) and ...
Lars Thiede +14 more
wiley +1 more source
Production of recombinant VP1-derived virus-like particles from novel human polyomaviruses in yeast
BMC Biotechnology, 2015 BackgroundEleven new human polyomaviruses (HPyVs) have been identified in the last decade. Serological studies show that these novel HPyVs sub-clinically infect humans at an early age.
M. Norkienė +5 more
semanticscholar +1 more source