Results 221 to 230 of about 39,685 (269)

Evidence of VP1 of duck hepatitis A type 1 virus as a target of neutralizing antibodies and involving receptor-binding activity

Virus Research, 2017
Chenxi Li, Yun Zhang
exaly   +2 more sources

Expression of Porcine Sapovirus VP1 Gene and VP1 Specific Monoclonal Antibody Production

Hybridoma, 2012
Sapovirus (SaV) is an agent of human and porcine gastroenteritis and a member of the family Caliciviridae. SaV has been classified based on VP1 full gene nucleotide sequences into five genogroups (GI-GV), among which GIII is known to infect pigs. The VP1 folds into two major domains designated S and P for the shell and protruding domain, respectively ...
Min-Sang, Ryu, Eun-Hye, Jung, Kyoung-Oh, Cho, Shien-Young, Kang   +3 more
openaire   +2 more sources

Nuclear localization of poliovirus capsid polypeptide VP1 expressed as a fusion protein with SV40-VP1

Gene, 1985
The poliovirus cDNA fragment coding for capsid polypeptide VP1 was inserted between the EcoRI and BamHI sites of SV40 DNA, generating a chimaeric gene in which the sequence of the 302 amino acids (aa) of poliovirus capsid polypeptide VP1 was placed downstream from that of the 94 N-terminal aa of SV40 capsid polypeptide VP1.
C, Wychowski, S, van der Werf, M, Girard
openaire   +2 more sources

The initiation region of the SV40 VP1 gene

Cell, 1976
The sequence of 15 nucleotides located at the 5' terminus of the plus strand of the SV40 Hind K fragment has been determined as (5') A-G-C-T-T-A-T-G-A-A-G-A-T-G-G (3'). The 3' on OH terminal G of this segment is part of the G-C-C codeword for the N terminal alanine of the VP1 protein.
A, Van de Voorde, R, Contreras, R, Rogiers, W, Fiers   +3 more
openaire   +2 more sources

Prokaryote expression of HPeV-1 VP1 protein, production of VP1 polyclonal antibody and the development of an ELISA

Journal of Virological Methods, 2012
The VP1 protein of human parechovirus (HPeV) plays a critical role in receptor binding based on its functional arginine-glycine-aspartic acid (RGD) motif region. Currently, only the neutralisation assay is used for seroepidemiological surveys of HPeVs. In the present study, the VP1 gene of HPeV-1 was cloned into the vector pET28a(+) to express the His ...
Ying, Yu, Tongling, Shan, Shaofeng, Zhu, Caixia, Zhu, Xiuguo, Hua, Chunmei, Wang   +5 more
openaire   +2 more sources

In vitro refolding of the structural protein VP1 of parvovirus B19 produces virus-like particles with functional VP1 unique region

Virology, 2022
Virus-like particles (VLPs) from Parvovirus B19 (B19V) can be obtained by the self-assembly of the structural proteins VP1 and VP2. It is possible to produce B19V VLPs either from VP2 or a mixture of VP1 and VP2, through its heterologous expression in eukaryotic cells. The difference between VP1 and VP2 protein is a tract of 227 residues located at the
Iris Ashanty Soto-Valerio, Maribel Cayetano-Cruz, Josefina Valadez-García, Patricia Guadarrama, Carmen Méndez, Ismael Bustos-Jaimes   +5 more
openaire   +2 more sources

Oral vaccine of recombinant Lactococcus lactis expressing the VP1 protein of duck hepatitis A virus type 3 induces mucosal and systemic immune responses.

Vaccine, 2019
Duck hepatitis A virus (DHAV) is the major pathogen of duck viral hepatitis, which has caused great economic losses to duck breeding industry. As an effective delivery tool for protein antigens, Lactococcus lactis (L.
S. Song, Pengfei Li, Ruihua Zhang, Junhao Chen, J. Lan, Shaoli Lin, G. Guo, Zhi-jing Xie, Shijin Jiang   +8 more
semanticscholar   +1 more source

โมโนโคลนอลแอนติบอดีต่อโปรตีน VP1 ของไวรัสโลหิตจางในไก่ และการระบุเอพิโทป

2023
โรคโลหิตจางในไก่ เกิดจากไวรัส Chicken anemia virus (CAV) ไก่ที่ติดไวรัส จะมีภาวะโลหิตจางอย่างรุนแรงตัวแคระแกร็น ต่อมธัยมัสฝ่อ เซลล์ไขกระดูกลดลง และภูมิคุ้มกันบกพร่อง โปรตีน VP1 เป็นโปรตีนโครงสร้างของไวรัส ทำหน้าที่ในการรวมกันเป็นอนุภาคไวรัส มีคุณสมบัติในการเป็นแอนติเจน CAV งานวิจัยก่อนหน้านี้ สามารถผลิตรีคอมบิแนนท์โปรตีน Δ60N_VP1 ของไวรัสโลหิตจางในไก ...
openaire   +1 more source

Unusual structural transition of antimicrobial VP1 peptide

Biophysical Chemistry, 2011
VP1 peptide, an active domain of m-calpain enzyme with antimicrobial activity is found to undergo an unusual conformational transition in trifluoroethanol (TFE) solvent. The nature of, and time dependent variations in, circular dichroism associated with the amide I vibrations, suggest that VP1 undergoes self-aggregation forming anti-parallel β-sheet ...
Ganesh, Shanmugam, Nsoki, Phambu, Prasad L, Polavarapu   +2 more
openaire   +2 more sources

Evidence that polyoma polypeptide VP1 is a serine protease

Virology, 1984
It has been shown that when purified polyoma (Py) virions are dissociated by incubation in 150 mM NaCl-50 mM Tris-HCl (pH 8.5) containing 1 mM EGTA and 3 mM DTT, two new polypeptides (MW 43.5K and 40K) are produced by proteolysis of virion polypeptide VP1. Proteolysis is blocked by diisopropyl fluorophosphonate (DFP) and phenylmethyl sulfonyl fluoride (
J H, Bowen, V, Chlumecky, P, d'Obrenan, J S, Colter   +3 more
openaire   +2 more sources