Results 231 to 240 of about 39,685 (269)
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Poliovirus serotype-specific VP1 sequencing primers
Journal of Virological Methods, 2011The Global Polio Laboratory Network routinely uses poliovirus-specific PCR primers and probes to determine the serotype and genotype of poliovirus isolates obtained as part of global poliovirus surveillance. To provide detailed molecular epidemiologic information, poliovirus isolates are further characterized by sequencing the ~900-nucleotide region ...
David R, Kilpatrick +10 more
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Pros and cons of VP1-specific maternal IgG for the protection of Enterovirus 71 infection
Vaccine, 2015Jae-Hyoung Song +2 more
exaly +2 more sources
2023
The Minor Planet Electronic Circulars contain information on unusual minor planets, routine data on comets and natural satellites, and occasional editorial announcements. They are published on behalf of Division F of the International Astronomical Union by the Minor Planet Center, Smithsonian Astrophysical Observatory, Cambridge, MA 02138, U.S.A.
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The Minor Planet Electronic Circulars contain information on unusual minor planets, routine data on comets and natural satellites, and occasional editorial announcements. They are published on behalf of Division F of the International Astronomical Union by the Minor Planet Center, Smithsonian Astrophysical Observatory, Cambridge, MA 02138, U.S.A.
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Synthesis of the SV40 viral polypeptide Vp1 during infection
Virology, 1978Abstract The synthesis of simian virus 40, SV40, polypeptides in permissively infected cells was studied by a very sensitive method utilizing 125I-labeled antibodies to identify and quantitate polypepties immobilized in gels. The synthesis of Vp1 begins about 24 hr postinfection, with the majority of the Vp1 being found in the nucleus.
H, Kasamatsu, P J, Flory
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Self-assembly of purified polyomavirus capsid protein VP1
Cell, 1986The polyomavirus major capsid protein VP1, purified after expression of the recombinant gene in E. coli, was isolated as oligomers resembling the dissociated capsomeres derived from viral capsids. Image analysis of low-dose electron micrographs demonstrates that these VP1 oligomers are exclusively pentamers.
D M, Salunke, D L, Caspar, R L, Garcea
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Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology, 2013
To express recombinant structural protein VP1 of enterovirus 71 (EV71) in E.coli and prepare anti-VP1 monoclonal antibodies (mAbs).With P1 gene as a template, EV71 VP1 gene was amplified by PCR and cloned into the expression vector pET-32a(+). After transformation into E.coli TG1, the positive clones were screened and sequenced.
Chen-yang, Li +7 more
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To express recombinant structural protein VP1 of enterovirus 71 (EV71) in E.coli and prepare anti-VP1 monoclonal antibodies (mAbs).With P1 gene as a template, EV71 VP1 gene was amplified by PCR and cloned into the expression vector pET-32a(+). After transformation into E.coli TG1, the positive clones were screened and sequenced.
Chen-yang, Li +7 more
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Insertion of MAP4-VP1 Peptide Into Lipid Monolayers and Bilayers
Biomedical Chromatography, 1997Myscibility of dipalmitoylphosphatidylcholine (DPPC) with dipalmitoylphosphatidylglycerol (DPPG), cardiolipine (CL) and dipalmitoylphosphatidylethanolamine (DPPE) was studied using monomolacular layers of different compositions. The influence of MAP4-VP1 peptide construct related to HAV protein in mixed monolayers of the above cited lipid components ...
Mestres, Concepció +3 more
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Genetic immunization is augmented by murine polyomavirus VP1 pseudocapsids
Vaccine, 2003To improve immune responses induced by DNA immunization, murine polyomavirus major capsid protein (VP1) pseudocapsids were complexed with a DNA plasmid encoding the p37 (p24 and p17) nucleocapsid proteins of the human immunodeficiency virus type 1 (HIV-1).
E, Rollman +10 more
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A complex between poliovirus RNA and the structural polypeptide VP1
Biochemical and Biophysical Research Communications, 1976Abstract The isolation of a complex between poliovirus RNA and the structural polypeptide VP1 is reported. This ribonucleopolypeptide (RNPP) is obtained by dissociation of poliovirus by urea and by subsequent centrifugation in urea containing sucrose gradients in hypotonic phosphate buffered saline. It sediments at about 45S and is sensitive to RNase
K J, Wiegers +2 more
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Expression of poliovirus capsid polypeptide VP1 in Escherichia coli
Gene, 1983A recombinant plasmid (pPV1-958) carrying a poliovirus cDNA insert corresponding to nucleotides 1-5750 of the poliovirus RNA genome was constructed through in vitro recombination of two plasmids carrying overlapping poliovirus cDNA inserts (Van der Werf et al., Proc. Natl. Acad. Sci. USA 78 (1981) 5983-5987). pPV1-958 was then cleaved with PstI and the
S, van der Werf +4 more
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