Results 31 to 40 of about 3,402,285 (161)

Truncated forms of viral VP2 proteins fused to EGFP assemble into fluorescent parvovirus-like particles

Journal of Nanobiotechnology, 2006
Fluorescence correlation spectroscopy (FCS) monitors random movements of fluorescent molecules in solution, giving information about the number and the size of for example nano-particles. The canine parvovirus VP2 structural protein as well as N-terminal
Vuento Matti, Korhonen Eila, Mäkelä Anna R, White Daniel, Cunningham Claire, Saloniemi Taija, Välilehto Outi, Toivola Jouni, Gilbert Leona, Oker-Blom Christian   +9 more
doaj   +1 more source

Single-Dose Vaccination of Recombinant Chimeric Newcastle Disease Virus (NDV) LaSota Vaccine Strain Expressing Infectious Bursal Disease Virus (IBDV) VP2 Gene Provides Full Protection against Genotype VII NDV and IBDV Challenge

Vaccines, 2021
Newcastle disease virus (NDV) and infectious bursal disease virus (IBDV) are the two most important and widespread viruses causing huge economic losses in the global poultry industry.
Qilong Qiao, Mingzhen Song, Congcong Song, Yihang Zhang, Xiangdong Wang, Qing Huang, Baiyu Wang, Panpan Yang, Shiyi Zhao, Yongtao Li, Zeng Wang, Jun Zhao   +11 more
doaj   +1 more source

Generation of Recombinant Porcine Parvovirus Virus-Like Particles in Saccharomyces cerevisiae and Development of Virus-Specific Monoclonal Antibodies

Journal of Immunology Research, 2014
Porcine parvovirus (PPV) is a widespread infectious virus that causes serious reproductive diseases of swine and death of piglets. The gene coding for the major capsid protein VP2 of PPV was amplified using viral nucleic acid extract from swine serum and
Paulius Lukas Tamošiūnas, Rasa Petraitytė-Burneikienė, Rita Lasickienė, Artiomas Akatov, Gabrielis Kundrotas, Vilimas Sereika, Raimundas Lelešius, Aurelija Žvirblienė, Kęstutis Sasnauskas   +8 more
doaj   +1 more source

The Merkel cell polyomavirus minor capsid protein.

PLoS Pathogens, 2013
The surface of polyomavirus virions is composed of pentameric knobs of the major capsid protein, VP1. In previously studied polyomavirus species, such as SV40, two interior capsid proteins, VP2 and VP3, emerge from the virion to play important roles ...
Rachel M Schowalter, Christopher B Buck
doaj   +1 more source

The relative expressed intensity of VP2 protein.

, 2015
(A) BV-S-ITRs-VP2; (B) BV-S-ITRs-VP2/4/3; (C) β-actin control. The VP2 protein intensity of recombinant baculovirus BV-VP2 and BV-VP2/4/3 was analyzed and the relative amount of VP2 protein was quantified.
Qi An (331241), Jingping Ge (636557), Wenxiang Ping (636561), Shanshan Song (500562), Dongni Gao (769100)   +4 more
core   +1 more source

Construction of cell line stably expressing IBDV VP 2 protein mutants

浙江大学学报. 农业与生命科学版, 2011
A couple of primers was designed on the base of VP2 gene sequence of infectious bursal disease virus (IBDV) HZ2 strain, and the VP2 gene was successfully cloned into eukaryotic expression vector pDsRed2-N1.
SUN Jing, WEI Yong-wei, ZHANG Xiao-dong, YU Lian   +3 more
doaj   +1 more source

Differing effects of the MCV VP2 protein on pseudovirus transduction.

, 2013
(A) Purified MCV pseudovirions with no VP2 (None) a low level of VP2 (Low) or a high level of VP2 (High) were analyzed by SDS-PAGE and SYPRO Ruby protein stain.
Christopher B. Buck (121726), Rachel M. Schowalter (121719)   +1 more
core   +1 more source

Mutational analysis of human norovirus VP2 elucidates critical molecular interactions for virus assembly

Journal of Virology
Human noroviruses (HuNoVs) are the leading cause of viral gastroenteritis, with ≥80% of infections caused by the GII genogroup. HuNoVs are non-enveloped, with an icosahedral capsid composed of 90 dimers of the major capsid protein VP1, which encloses a ...
Janam J. Dave, Sue E. Crawford, Robert L. Atmar, Khalil Ettayebi, B. V. Venkataram Prasad, Mary K. Estes   +5 more
doaj   +1 more source

What Makes an “Ideal” Cell Line for Recombinant Adeno‐Associated Virus Production?

Biotechnology and Bioengineering, EarlyView.
Several host cell types have been used to produce rAAVs to date. Cell line‐specific traits that are beneficial in the context of rAAV manufacturing are reviewed here, with the goal of developing a consensus on the ‘ideal’ characteristics that an rAAV production host should possess (created with Biorender.com).
James Conheady, Nicholas Donohue, Alexandra Bogdanovic, Sharon Davin, Brian Glennon, Niall Barron, C. Mark Smales   +6 more
wiley   +1 more source

Enhancing Adeno‐Associated Virus 2 Capsid Engineering Through Co‐Evolutionary Coupling

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Adeno‐associated virus (AAV) has emerged as a leading platform for gene therapy. However, to unlock the full potential, their manufacturing yields, stability, and efficacy must all be improved. Rational design is limited by poor predictability and the potential impact of mutations on multiple important vector properties.
Sirimar Laosinwattana, Yiwen Li, Braulio Carrillo Sanchez, Ayesha Khan, Paul A. Dalby   +4 more
wiley   +1 more source