Engineering Xylose Isomerase and Reductase Pathways in Yarrowia lipolytica for Efficient Lipid Production [PDF]
Microbial BiotechnologyXylose is a common monosaccharide in lignocellulosic residues that Yarrowia lipolytica cannot naturally metabolise for lipid production and therefore, heterologous xylose metabolic pathways must be engineered in this yeast to facilitate its consumption ...
Isabel De La Torre +4 more
doaj +4 more sources
Engineering Saccharomyces Cerevisiae With Novel Functional Xylose Isomerases From Rumen Microbiota for Enhanced Biofuel Production. [PDF]
Biotechnol JGraphical Abstract and Lay Summary This study identified novel xylose isomerases (XIs) from ruminal microbiota using omics data. Five Bacillota XIs were efficiently expressed in Saccharomyces cerevisiae, enabling effective conversion of xylose into xylulose and subsequent ethanol production.
Vargas BO +8 more
europepmc +3 more sources
Structure-based directed evolution improves S. cerevisiae growth on xylose by influencing in vivo enzyme performance [PDF]
Biotechnology for Biofuels, 2020 Background Efficient bioethanol production from hemicellulose feedstocks by Saccharomyces cerevisiae requires xylose utilization. Whereas S. cerevisiae does not metabolize xylose, engineered strains that express xylose isomerase can metabolize xylose by ...
Misun Lee +4 more
doaj +2 more sources
Comparing the xylose reductase/xylitol dehydrogenase and xylose isomerase pathways in arabinose and xylose fermenting
Biotechnology for Biofuels, 2008 Background Ethanolic fermentation of lignocellulosic biomass is a sustainable option for the production of bioethanol. This process would greatly benefit from recombinant Saccharomyces cerevisiae strains also able to ferment, besides the hexose sugar ...
Hahn-Hägerdal Bärbel +2 more
doaj +5 more sources
A novel d-xylose isomerase from the gut of the wood feeding beetle Odontotaenius disjunctus efficiently expressed in Saccharomyces cerevisiae [PDF]
Scientific Reports, 2021 Carbohydrate rich substrates such as lignocellulosic hydrolysates remain one of the primary sources of potentially renewable fuel and bulk chemicals. The pentose sugar d-xylose is often present in significant amounts along with hexoses.
Paulo César Silva +5 more
doaj +2 more sources
Directed evolution and secretory expression of xylose isomerase for improved utilisation of xylose in Saccharomyces cerevisiae [PDF]
Biotechnology for Biofuels, 2021 Background Xylose contained in lignocellulosic biomass is an attractive carbon substrate for economically viable conversion to bioethanol. Extensive research has been conducted on xylose fermentation using recombinant Saccharomyces cerevisiae expressing ...
Jung-Hoon Bae +4 more
doaj +2 more sources
Safety evaluation of the food enzyme xylose isomerase from the genetically modified Streptomyces rubiginosus strain DP‐Pzn37 [PDF]
EFSA Journal, 2020 The food enzyme is a d‐xylose aldose‐ketose‐isomerase (EC 5.3.1.5) produced with the genetically modified Streptomyces rubiginosus strain DP‐Pzn37 by Danisco US Inc. Although the production strain contains antibiotic resistance genes, the food enzyme was
EFSA Panel on Food Contact Materials, Enzymes and Processing Aids (CEP) +27 more
doaj +2 more sources
Frontiers in Bioengineering and Biotechnology, 2020 Pseudomonas putida KT2440 is a well-established chassis in industrial biotechnology. To increase the substrate spectrum, we implemented three alternative xylose utilization pathways, namely the Isomerase, Weimberg, and Dahms pathways.
Isabel Bator +8 more
doaj +2 more sources
Metabolomic and (13)C-metabolic flux analysis of a xylose-consuming Saccharomyces cerevisiae strain expressing xylose isomerase. [PDF]
Biotechnol Bioeng, 2015 Over the past two decades, significant progress has been made in the engineering of xylose-consuming Saccharomyces cerevisiae strains for production of lignocellulosic biofuels.
Wasylenko TM, Stephanopoulos G.
europepmc +3 more sources
Heterologous xylose isomerase pathway and evolutionary engineering improve xylose utilization in Saccharomyces cerevisiae [PDF]
Frontiers in Microbiology, 2015 Xylose utilization is one key issue for the bioconversion of lignocelluloses. It is promising approach to engineer heterologous pathway for xylose utilization in Saccharomyces cerevisiae.
Xin eQi +5 more
doaj +2 more sources