Engineering Saccharomyces Cerevisiae With Novel Functional Xylose Isomerases From Rumen Microbiota for Enhanced Biofuel Production. [PDF]
Biotechnol JGraphical Abstract and Lay Summary This study identified novel xylose isomerases (XIs) from ruminal microbiota using omics data. Five Bacillota XIs were efficiently expressed in Saccharomyces cerevisiae, enabling effective conversion of xylose into xylulose and subsequent ethanol production.
Vargas BO +8 more
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Structural Analysis of Xylose Isomerase from Streptomyces avermitilis [PDF]
CrystalsXylose isomerase (XI, also known as glucose isomerase) is an oxidoreductase that interconverts aldoses and ketoses. XI catalyzes the reversible isomerization of D-glucose and D-xylose into D-fructose and D-xylulose, respectively.
Ki Hyun Nam
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Positive selection of efficient ethanol producers from xylose at 45 °C in the yeast Ogataea polymorpha [PDF]
Scientific ReportsThis research presents a method for the positive selection of mutants with improved xylose and L-arabinose fermentation in the thermotolerant, naturally xylose-utilizing yeast Ogataea polymorpha which is based on isolation of the mutants growing on L ...
Roksolana Vasylyshyn +3 more
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GCB BioenergyMetabolic engineering of Saccharomyces cerevisiae has enabled xylose‐fermenting yeast strains. However, the bioavailability dilemma of xylose has become the core bottleneck restricting the economy of lignocellulose.
Si Xu +5 more
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Comparison of the xylose reductase-xylitol dehydrogenase and the xylose isomerase pathways for xylose fermentation by recombinant
Microbial Cell Factories, 2007 Background Two heterologous pathways have been used to construct recombinant xylose-fermenting Saccharomyces cerevisiae strains: i) the xylose reductase (XR) and xylitol dehydrogenase (XDH) pathway and ii) the xylose isomerase (XI) pathway.
Hahn-Hägerdal Bärbel +3 more
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Genome sequence of carboxylesterase, carboxylase and xylose isomerase producing alkaliphilic haloarchaeon Haloterrigena turkmenica WANU15 [PDF]
Genomics Data, 2016 We report draft genome sequence of Haloterrigena turkmenica strain WANU15, isolated from Soda Lake. The draft genome size is 2,950,899 bp with a G + C content of 64% and contains 49 RNA sequence.
Samy Selim, Nashwa Hagagy
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Engineering the substrate specificity of xylose isomerase [PDF]
Protein Engineering Design and Selection, 2005 Xylose isomerase (XI) catalyzes the isomerization and epimerization of hexoses, pentoses and tetroses. In order to clarify the reasons for the low reaction efficiency of a pentose sugar, L-arabinose, we determined the crystal structure of Streptomyces rubiginosus XI complexed with L-arabinose.
Johanna Karimäki +6 more
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Mining Xylose Isomerase Producing Microbes [PDF]
Madras Agricultural Journal, 2020 An aerobic, rod shaped, mesophilic, gram positive xylitol producing bacterial isolates were made from different enriched substrates. Xylose isomerase is an enzyme that catalyzes the interconversion of D-xylose and D-xylulose. The microbes producing xylulose from xylose via xylose isomerase enzyme were screened for the xylose isomerase activity based on
Meyyappan Geetha Valliammai +3 more
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Mutations in PMR1 stimulate xylose isomerase activity and anaerobic growth on xylose of engineered Saccharomyces cerevisiae by influencing manganese homeostasis. [PDF]
Sci Rep, 2017 Verhoeven MD +7 more
europepmc +3 more sources
FermentationA biocatalyst has been developed for application in the simultaneous isomerization and fermentation (SIF) of xylose, which could enable operation in repeated batches and the use of xylose from biomass hemicellulose for the production of second-generation
Márcio D. N. Ramos +4 more
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