Xylose fermentation efficiency of industrial Saccharomyces cerevisiae yeast with separate or combined xylose reductase/xylitol dehydrogenase and xylose isomerase pathways [PDF]
Biotechnology for Biofuels, 2019 Background Xylose isomerase (XI) and xylose reductase/xylitol dehydrogenase (XR/XDH) pathways have been extensively used to confer xylose assimilation capacity to Saccharomyces cerevisiae and tackle one of the major bottlenecks in the attainment of ...
Joana T. Cunha +4 more
doaj +3 more sources
Molecular evolutionary engineering of xylose isomerase to improve its catalytic activity and performance of micro-aerobic glucose/xylose co-fermentation in Saccharomyces cerevisiae. [PDF]
Biotechnol Biofuels, 2019 BackgroundExpression of d-xylose isomerase having high catalytic activity in Saccharomyces cerevisiae (S. cerevisiae) is a prerequisite for efficient and economical production of bioethanol from cellulosic biomass.
Seike T +5 more
europepmc +4 more sources
Structural Analysis of Xylose Isomerase from Streptomyces avermitilis
CrystalsXylose isomerase (XI, also known as glucose isomerase) is an oxidoreductase that interconverts aldoses and ketoses. XI catalyzes the reversible isomerization of D-glucose and D-xylose into D-fructose and D-xylulose, respectively.
Ki Hyun Nam
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Screening and evolution of a novel protist xylose isomerase from the termite Reticulitermes speratus for efficient xylose fermentation in Saccharomyces cerevisiae. [PDF]
Biotechnol Biofuels, 2017 BackgroundThe yeast Saccharomyces cerevisiae, a promising host for lignocellulosic bioethanol production, is unable to metabolize xylose. In attempts to confer xylose utilization ability in S. cerevisiae, a number of xylose isomerase (XI) genes have been
Katahira S +8 more
europepmc +4 more sources
Conversion of an inactive xylose isomerase into a functional enzyme by co-expression of GroEL-GroES chaperonins in Saccharomyces cerevisiae. [PDF]
BMC Biotechnol, 2017 BackgroundSecond-generation ethanol production is a clean bioenergy source with potential to mitigate fossil fuel emissions. The engineering of Saccharomyces cerevisiae for xylose utilization is an essential step towards the production of this biofuel ...
Temer B +9 more
europepmc +3 more sources
Clean Technologies, 2022 Xylose Isomerase (XI) is an intramolecular oxidoreductase enzyme and catalyzes the reversible conversion of ketoses and aldoses in addition to the bioconversion of ethanol from xylose in the production of bioethanol from hemicellulose.
Maurya Sharma +5 more
doaj +2 more sources
Mining Xylose Isomerase Producing Microbes [PDF]
Madras Agricultural Journal, 2020 An aerobic, rod shaped, mesophilic, gram positive xylitol producing bacterial isolates were made from different enriched substrates. Xylose isomerase is an enzyme that catalyzes the interconversion of D-xylose and D-xylulose. The microbes producing xylulose from xylose via xylose isomerase enzyme were screened for the xylose isomerase activity based on
Geetha Valliammai Meyyappan +3 more
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Metabolomic and (13)C-metabolic flux analysis of a xylose-consuming Saccharomyces cerevisiae strain expressing xylose isomerase. [PDF]
Biotechnol Bioeng, 2015 Over the past two decades, significant progress has been made in the engineering of xylose-consuming Saccharomyces cerevisiae strains for production of lignocellulosic biofuels.
Wasylenko TM, Stephanopoulos G.
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Frontiers in Bioengineering and Biotechnology, 2020 Pseudomonas putida KT2440 is a well-established chassis in industrial biotechnology. To increase the substrate spectrum, we implemented three alternative xylose utilization pathways, namely the Isomerase, Weimberg, and Dahms pathways.
Isabel Bator +8 more
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Xylose Isomerase Depletion Enhances Virulence of Xanthomonas citri subsp. citri in Citrus aurantifolia. [PDF]
Int J Mol Sci, 2023 Citrus canker, caused by the bacterium Xanthomonas citri (Xcc), is one of the most devastating diseases for the citrus industry. Xylose is a constituent of the cell wall of plants, and the ability of Xcc to use this carbohydrate may play a role in ...
Alexandrino AV +8 more
europepmc +2 more sources