Journal of General and Applied Microbiology, 2022 Researchers continue to search for efficient processes to reduce the production costs of rare sugars. In this paper, we report a novel D-xylose isomerase from Shinella zoogloeoides NN6 (SzXI) and its application for efficient rare sugar production ...
Takuma Suzuki, Kenji Morimoto
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Engineering Xylose Isomerase for Industrial Applications
CatalystsXylose isomerase (XI), also known as glucose isomerase, is an aldose isomerase that converts D-glucose to D-fructose and D-xylose to D-xylulose. This enzyme is widely used in the production of high-fructose corn syrup and bioethanol. Enhancing the efficiency of XI is critical for its use in industrial applications.
Ki Hyun Nam
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FermentationA biocatalyst has been developed for application in the simultaneous isomerization and fermentation (SIF) of xylose, which could enable operation in repeated batches and the use of xylose from biomass hemicellulose for the production of second-generation
Márcio D. N. Ramos +4 more
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Comparison of the xylose reductase-xylitol dehydrogenase and the xylose isomerase pathways for xylose fermentation by recombinant
Microbial Cell Factories, 2007 Background Two heterologous pathways have been used to construct recombinant xylose-fermenting Saccharomyces cerevisiae strains: i) the xylose reductase (XR) and xylitol dehydrogenase (XDH) pathway and ii) the xylose isomerase (XI) pathway.
Hahn-Hägerdal Bärbel +3 more
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Positive selection of efficient ethanol producers from xylose at 45 °C in the yeast Ogataea polymorpha [PDF]
Scientific ReportsThis research presents a method for the positive selection of mutants with improved xylose and L-arabinose fermentation in the thermotolerant, naturally xylose-utilizing yeast Ogataea polymorpha which is based on isolation of the mutants growing on L ...
Roksolana Vasylyshyn +3 more
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Engineering metal specificity in glucose (xylose) isomerase [PDF]
Acta Crystallographica Section A Foundations of Crystallography, 1993 J. Janin
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Engineering Saccharomyces Cerevisiae With Novel Functional Xylose Isomerases From Rumen Microbiota for Enhanced Biofuel Production. [PDF]
Biotechnol JVargas BO +8 more
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Structural knowledge or X-ray damage? A case study on xylose isomerase illustrating both. [PDF]
J Synchrotron Radiat, 2019 Xylose isomerase (XI) is an industrially important metalloprotein studied for decades. Its reaction mechanism has been postulated to involve movement of the catalytic metal cofactor to several different conformations.
Taberman H +4 more
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Understanding xylose isomerase from Burkholderia cenocepacia: insights into structure and functionality for ethanol production. [PDF]
AMB Express, 2019 The inability of the yeast Saccharomyces cerevisiae to produce ethanol from xylose has hampered the biofuel production from lignocellulosic biomass.
Vieira IPV +8 more
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Metal Dependence of the Xylose Isomerase from Piromyces sp. E2 Explored by Activity Profiling and Protein Crystallography. [PDF]
Biochemistry, 2017 Xylose isomerase from Piromyces sp. E2 (PirXI) can be used to equip Saccharomyces cerevisiae with the capacity to ferment xylose to ethanol. The biochemical properties and structure of the enzyme have not been described even though its metal content ...
Lee M +5 more
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