Mutations in PMR1 stimulate xylose isomerase activity and anaerobic growth on xylose of engineered Saccharomyces cerevisiae by influencing manganese homeostasis. [PDF]
Sci Rep, 2017 Combined overexpression of xylulokinase, pentose-phosphate-pathway enzymes and a heterologous xylose isomerase (XI) is required but insufficient for anaerobic growth of Saccharomyces cerevisiae on d-xylose.
Verhoeven MD +7 more
europepmc +2 more sources
Engineering the xylose metabolism in Schizochytrium sp. to improve the utilization of lignocellulose
Biotechnology for Biofuels and Bioproducts, 2022 Background Schizochytrium sp. is a heterotrophic, oil-producing microorganism that can efficiently produce lipids. However, the industrial production of bulk chemicals using Schizochytrium sp.
Ling-Ru Wang +7 more
doaj +1 more source
Letters in Applied Microbiology, 2022 The current climate crisis demands replacement of fossil energy sources with sustainable alternatives. In this scenario, second‐generation bioethanol, a product of lignocellulosic biomass fermentation, represents a more sustainable alternative.
Gabriel de Souza Colombo +8 more
semanticscholar +1 more source
Biotechnology for Biofuels, 2020 Background Efficient bioethanol production from hemicellulose feedstocks by Saccharomyces cerevisiae requires xylose utilization. Whereas S. cerevisiae does not metabolize xylose, engineered strains that express xylose isomerase can metabolize xylose by ...
Misun Lee +4 more
doaj +1 more source
PLoS ONE, 2020 Xylose, the second most abundant sugar in lignocellulosic biomass hydrolysates, can be fermented by Saccharomyces cerevisiae expressing one of two heterologous xylose pathways: a xylose oxidoreductase pathway and a xylose isomerase pathway.
Deokyeol Jeong +7 more
doaj +1 more source
Engineering and two-stage evolution of a lignocellulosic hydrolysate-tolerant Saccharomyces cerevisiae strain for anaerobic fermentation of xylose from AFEX pretreated corn stover. [PDF]
PLoS ONE, 2014 The inability of the yeast Saccharomyces cerevisiae to ferment xylose effectively under anaerobic conditions is a major barrier to economical production of lignocellulosic biofuels. Although genetic approaches have enabled engineering of S. cerevisiae to
Lucas S Parreiras +31 more
doaj +1 more source
Glucose Isomerase: Functions, Structures, and Applications
Applied Sciences, 2022 Glucose isomerase (GI, also known as xylose isomerase) reversibly isomerizes D-glucose and D-xylose to D-fructose and D-xylulose, respectively. GI plays an important role in sugar metabolism, fulfilling nutritional requirements in bacteria.
Ki Hyun Nam
doaj +1 more source
Development of a D-xylose fermenting and inhibitor tolerant industrial Saccharomyces cerevisiae strain with high performance in lignocellulose hydrolysates using metabolic and evolutionary engineering [PDF]
, 2013 Background: The production of bioethanol from lignocellulose hydrolysates requires a robust, D-xylose-fermenting and inhibitor-tolerant microorganism as catalyst.
Boles, Eckhard +13 more
core +1 more source
Engineering the substrate specificity of xylose isomerase [PDF]
Protein Engineering Design and Selection, 2005 Xylose isomerase (XI) catalyzes the isomerization and epimerization of hexoses, pentoses and tetroses. In order to clarify the reasons for the low reaction efficiency of a pentose sugar, L-arabinose, we determined the crystal structure of Streptomyces rubiginosus XI complexed with L-arabinose.
Johanna, Karimäki +6 more
openaire +2 more sources
GCB Bioenergy, 2020 Lignocellulosic biomass shows high potential as a renewable feedstock for use in biodiesel production via microbial fermentation. Yarrowia lipolytica, an emerging oleaginous yeast, has been engineered to efficiently convert xylose, the second most ...
Sang Do Yook +6 more
doaj +1 more source