Analysis of a conserved cellulase transcriptional regulator reveals inducer-independent production of cellulolytic enzymes in Neurospora crassa. [PDF]
, 2013 Cellulose is recalcitrant to deconstruction to glucose for use in fermentation strategies for biofuels and chemicals derived from lignocellulose. In Neurospora crassa, the transcriptional regulator, CLR-2, is required for cellulolytic gene expression and
Coradetti, Samuel +2 more
core +2 more sources
D-Xylose fermentation to ethanol bySchizosaccharomyces pombe cloned with xylose isomerase gene [PDF]
Biotechnology Letters, 1986 Schizosaccharomycespombe cloned with the xylose isomerase gene from E. coli is able to grow on YNB and YMP broths containing xylose as the sole carbon source. This yeast can ferment D-xylose to ethanol directly; however, the ethanol production rate and the yield were dependent on the nitrogen source.
E. Cheng Chan, P. P. Ueng, L. Chen
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Biotechnology for Biofuels, 2018 Background Engineered strains of Saccharomyces cerevisiae have significantly improved the prospects of biorefinery by improving the bioconversion yields in lignocellulosic bioethanol production and expanding the product profiles to include advanced ...
Phuong Tran Nguyen Hoang +4 more
doaj +1 more source
Label-free shotgun proteomics and metabolite analysis reveal a significant metabolic shift during citrus fruit development. [PDF]
, 2011 Label-free LC-MS/MS-based shot-gun proteomics was used to quantify the differential protein synthesis and metabolite profiling in order to assess metabolic changes during the development of citrus fruits.
Blumwald, Eduardo +8 more
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Crystallographic Studies of d-Xylose Isomerase
Journal of Biological Chemistry, 1974 In order to emphasize the similarity of the unit cell and of the diffraction patterns, this convention is only followed for Form B. The estimated error in unit cell measurements is 0.05 A for Form A crystals and 0.15 A for Form B crystals. The determined densities were reproducible within 0.02 g per cc.
H M, Berman +3 more
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Functional characterization of a xylose transporter in Aspergillus nidulans [PDF]
, 2014 BACKGROUND: The production of bioethanol from lignocellulosic feedstocks will only become economically feasible when the majority of cellulosic and hemicellulosic biopolymers can be efficiently converted into bioethanol.
Brown, Neil Andrew +8 more
core +6 more sources
Anomeric specificity of D-xylose isomerase
Biochemistry, 1992 Crystal structures of complexes of D-xylose isomerase with deoxysugars have been determined. Deoxynojirimycin is a structural analogue of alpha-pyranose and mimics the binding of these aldose substrates. The structure of this complex supports the hypothesis that an imidazole group catalyzes ring opening of the pyranose.
Collyer, C +7 more
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Fermentation, 2018 Cost-effective production of cellulosic ethanol requires robust microorganisms for rapid co-fermentation of glucose and xylose. This study aims to develop a recombinant diploid xylose-fermenting Saccharomyces cerevisiae strain for efficient conversion of
Tingting Liu +2 more
doaj +1 more source
Process intensification through microbial strain evolution: mixed glucose-xylose fermentation in wheat straw hydrolyzates by three generations of recombinant Saccharomyces cerevisiae [PDF]
, 2014 BACKGROUND: Lignocellulose hydrolyzates present difficult substrates for ethanol production by the most commonly applied microorganism in the fermentation industries, Saccharomyces cerevisiae.
Klimacek, Mario +6 more
core +2 more sources
Xylose (glucose) isomerase gene from the thermophile Thermus thermophilus: cloning, sequencing, and comparison with other thermostable xylose isomerases [PDF]
Journal of Bacteriology, 1991 The xylose isomerase gene from the thermophile Thermus thermophilus was cloned by using a fragment of the Streptomyces griseofuscus gene as a probe. The complete nucleotide sequence of the gene was determined. T. thermophilus is the most thermophilic organism from which a xylose isomerase gene has been cloned and characterized.
K, Dekker +3 more
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