Results 131 to 140 of about 7,321 (167)
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Putative xylose and arabinose reductases in Saccharomyces cerevisiae
Yeast, 2002AbstractSaccharomyces cerevisiae mutants, in which open reading frames (ORFs) displaying similarity to the aldo‐keto reductase GRE3 gene have been deleted, were investigated regarding their ability to utilize xylose and arabinose. Reduced xylitol formation from D‐xylose in gre3 mutants of S. cerevisiae suggests that Gre3p is the major D‐xylose‐reducing
K L, Träff +2 more
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Extraction by Reversed Micelles of the Intracellular Enzyme Xylose Reductase
Applied Biochemistry and Biotechnology, 2001Xylose reductase enzyme (EC 1.1.1.21) produced by Candida guilliermondii in sugarcane bagasse was extracted by reversed micelles of N-benzyl-N-dodecyl-N-bis (2-hydroxyethyl) ammonium chloride cationic surfactant. An experimental design was employed to evaluate the influences of the following factors on the enzyme extraction: temperature, cosolvent, and
E V, Cortez +4 more
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Xylose metabolism in Pachysolen tannophilus: purification and properties of xylose reductase
Canadian Journal of Microbiology, 1984Xylose reductase (xylitol: NADP oxidoreductase, EC 1.1.1.139) has been purified from D-xylose grown cells of the yeast Pachysolen tannophilus by application of DEAE-cellulose ion exchange chromatography, 2′,5′-ADP-Sepharose affinity chromatography, Biogel P200 gel filtration, and dextran blue Sepharose chromatography to approximately 95% homogeneity ...
Günther Ditzelmüller +3 more
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Biotechnology Journal, 2009
AbstractMetabolic engineering of Saccharomyces cerevisiae for xylose fermentation has often relied on insertion of a heterologous pathway consisting of nicotinamide adenine dinucleotide (phosphate) NAD(P)H‐dependent xylose reductase (XR) and NAD+‐dependent xylitol dehydrogenase (XDH).
Stefan, Krahulec +2 more
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AbstractMetabolic engineering of Saccharomyces cerevisiae for xylose fermentation has often relied on insertion of a heterologous pathway consisting of nicotinamide adenine dinucleotide (phosphate) NAD(P)H‐dependent xylose reductase (XR) and NAD+‐dependent xylitol dehydrogenase (XDH).
Stefan, Krahulec +2 more
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Optimization of Xylose Reductase production from Citrobacter sp.
Research Journal of Biotechnology, 2021Xylitol is a poly-hydroxy straight-chain five-carbon alcohol that can replace sugar in daily uses without any side effects. Lowered risk of dental carries and bone demineralization further support its involvement in a healthy lifestyle. In addition, its role in the synthesis of various commercial products like glycol, ethanol, and resins etc. increases
Rashmi Niranjan +2 more
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Yeast, 1996
A xylose reductase gene was isolated from the xylose-fermenting yeast Pachysolen tannophilus as a cDNA clone by selecting clones that hybridized specifically to xylose-inducible messenger RNA. Use of the cDNA clone as a probe in Northern hybridizations identified a xylose-inducible mRNA species large enough to encode a 36 kDa xylose reductase protein ...
P L, Bolen, G T, Hayman, H S, Shepherd
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A xylose reductase gene was isolated from the xylose-fermenting yeast Pachysolen tannophilus as a cDNA clone by selecting clones that hybridized specifically to xylose-inducible messenger RNA. Use of the cDNA clone as a probe in Northern hybridizations identified a xylose-inducible mRNA species large enough to encode a 36 kDa xylose reductase protein ...
P L, Bolen, G T, Hayman, H S, Shepherd
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The Production and Properties of a New Xylose Reductase from Fungus Neurospora crassa
Applied Biochemistry and Biotechnology, 1998Neurospora crassa XI was found to ferment xylose and glucose simultaneously. Xylose was the appropriate inducer for the production of xylose reductase that had two isoenzymes designated as EI and EII. Both EI and EII, which were purified by affinity chromatography, had NADPH-dependent xylose reductase activities.
X, Zhao, P, Gao, Z, Wang
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Process Biochemistry, 2020
Abstract The co-production of xylitol and ethanol from agricultural straw has more economic advantages than the production of ethanol only. Saccharomyces cerevisiae, the most widely used ethanol-producing yeast, can be genetically engineered to ferment xylose to xylitol.
Bai-Xue Yang +5 more
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Abstract The co-production of xylitol and ethanol from agricultural straw has more economic advantages than the production of ethanol only. Saccharomyces cerevisiae, the most widely used ethanol-producing yeast, can be genetically engineered to ferment xylose to xylitol.
Bai-Xue Yang +5 more
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Applied Biochemistry and Biotechnology, 2003
We changed the fluxes of xylose metabolites in recombinant Saccharomyces cerevisiae by manipulating expression of Pichia stipitis genes (XYL1 and XYL2) coding for xylose reductase (XR) and xylitol dehydrogenase (XDH), respectively. XYL1 copy number was kept constant by integrating it into the chromosome.
Yong-Su, Jin, Thomas W, Jeffries
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We changed the fluxes of xylose metabolites in recombinant Saccharomyces cerevisiae by manipulating expression of Pichia stipitis genes (XYL1 and XYL2) coding for xylose reductase (XR) and xylitol dehydrogenase (XDH), respectively. XYL1 copy number was kept constant by integrating it into the chromosome.
Yong-Su, Jin, Thomas W, Jeffries
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Chemico-Biological Interactions, 2001
The primary structure of the aldose xylose reductase from Candida tenuis (CtAR) is shown to be 39% identical to that of human aldose reductase (hAR). The catalytic tetrad of hAR is completely conserved in CtAR (Tyr51, Lys80, Asp46, His113). The amino acid residues involved in binding of NADPH by hAR (D.K.
B, Nidetzky +3 more
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The primary structure of the aldose xylose reductase from Candida tenuis (CtAR) is shown to be 39% identical to that of human aldose reductase (hAR). The catalytic tetrad of hAR is completely conserved in CtAR (Tyr51, Lys80, Asp46, His113). The amino acid residues involved in binding of NADPH by hAR (D.K.
B, Nidetzky +3 more
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