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Characterization of a xylitol dehydrogenase from Aspergillus flavus and its application in l-xylulose production [PDF]

open access: yesFrontiers in Bioengineering and Biotechnology, 2022
An NAD+-dependent xylitol dehydrogenase from A. flavus (AfXDH) was cloned and successfully expressed in Escherichia coli. AfXDH gene sequence revealed an open reading frame of 1,110 bp, encoding a polypeptide of 369 amino acids with a calculated ...
Anurag Kumar   +7 more
doaj   +2 more sources

Acquisition of a gene cluster in Clostridioides difficile PCR ribotype 023 strains enables xylitol utilization [PDF]

open access: yesmSphere
Hundreds of ribotypes of the gastrointestinal pathogen Clostridioides difficile have emerged over the last three decades, yet the factors driving their emergence are poorly understood.
Katherine J. Wozniak   +6 more
doaj   +2 more sources

Improving xylitol production by semi-rational engineering of xylitol dehydrogenase and optimizing cofactor regeneration in Gluconobacter oxydans [PDF]

open access: yesBMC Biotechnology
Background Xylitol, a widely used sugar substitute in food and medicine, can be produced through microbial bioconversion using glucose as the primary substrate. In this process, a critical factor limiting xylitol production is the relatively low activity
Jun-Hao Su   +4 more
doaj   +2 more sources

Xylose fermentation efficiency of industrial Saccharomyces cerevisiae yeast with separate or combined xylose reductase/xylitol dehydrogenase and xylose isomerase pathways [PDF]

open access: yesBiotechnology for Biofuels, 2019
Background Xylose isomerase (XI) and xylose reductase/xylitol dehydrogenase (XR/XDH) pathways have been extensively used to confer xylose assimilation capacity to Saccharomyces cerevisiae and tackle one of the major bottlenecks in the attainment of ...
Joana T. Cunha   +4 more
doaj   +2 more sources

Metabolic engineering for sustainable xylitol production from diverse carbon sources in Pichia pastoris [PDF]

open access: yesMicrobial Cell Factories
Xylitol, known for its health benefits, is a valuable compound in the food and pharmaceutical industries. However, conventional chemical production methods are often unsustainable for large-scale applications, prompting the need for alternative ...
Xiaocong Lu   +8 more
doaj   +2 more sources

Heterologous expression of Spathaspora passalidarum xylose reductase and xylitol dehydrogenase genes improved xylose fermentation ability of Aureobasidium pullulans [PDF]

open access: yesMicrobial Cell Factories, 2018
Background Aureobasidium pullulans is a yeast-like fungus that can ferment xylose to generate high-value-added products, such as pullulan, heavy oil, and melanin.
Jian Guo   +4 more
doaj   +2 more sources

Data for rapid ethanol production at elevated temperatures by engineered thermotolerant Kluyveromyces marxianus via the NADP(H)-preferring xylose reductase–xylitol dehydrogenase pathway [PDF]

open access: yesData in Brief, 2015
A thermo-tolerant NADP(H)-preferring xylose pathway was constructed in Kluyveromyces marxianus for ethanol production with xylose at elevated temperatures (Zhang et al., 2015 [25]).
Biao Zhang   +5 more
doaj   +2 more sources

Stepwise metabolic engineering of Candida tropicalis for efficient xylitol production from xylose mother liquor

open access: yesMicrobial Cell Factories, 2021
Background Commercial xylose purification produces xylose mother liquor (XML) as a major byproduct, which has become an inexpensive and abundant carbon source.
Lihua Zhang   +5 more
doaj   +1 more source

Development of engineered Candida tropicalis strain for efficient corncob-based xylitol-ethanol biorefinery

open access: yesMicrobial Cell Factories, 2023
Background Xylitol has a wide range of applications in the pharmaceuticals, cosmetic, food and beverage industry. Microbial xylitol production reduces the risk of contamination and is considered as environment friendly and sustainable compared to the ...
Anup Kumar Singh   +6 more
doaj   +1 more source

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