Development of a xylitol biosensor composed of xylitol dehydrogenase and diaphorase
In preparation for the development of a xylitol biosensor, the xylitol dehydrogenase of Candida tropicalis IFO 0618 was partially purified and characterized. The optimal pH and temperature of the xylitol dehydrogenase were pH 8.0 and 50°C, respectively.
K, Takamizawa +4 more
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Production of Xylitol from
ABSTRACT Xylitol dehydrogenase (XDH) is one of the key enzymes in d -xylose metabolism, catalyzing the oxidation of xylitol to d -xylulose. Two copies of the XYL2 gene encoding XDH in the diploid yeast Candida tropicalis were ...
Ko, BS Byoung Sam Ko +2 more
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Comparing the xylose reductase/xylitol dehydrogenase and xylose isomerase pathways in arabinose and xylose fermenting
Background Ethanolic fermentation of lignocellulosic biomass is a sustainable option for the production of bioethanol. This process would greatly benefit from recombinant Saccharomyces cerevisiae strains also able to ferment, besides the hexose sugar ...
Hahn-Hägerdal Bärbel +2 more
doaj +2 more sources
Evidence that the gene YLR070c of Saccharomyces cerevisiae encodes a xylitol dehydrogenase [PDF]
The open reading frame YLR070c of Saccharomyces cerevisiae has high sequence similarity to S. cerevisiae sorbitol dehydrogenase and to xylitol dehydrogenase of Pichia stipitis. Overexpression of this open reading frame in S. cerevisiae resulted in xylitol dehydrogenase activity. The enzyme is specific for NADH.
Richard, Peter +2 more
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Dual relationships of xylitol and alcohol dehydrogenases in families of two protein types [PDF]
Xylitol dehydrogenase encoded by gene XYL2 from Pichia stipitis is a member of the medium‐chain alcohol dehydrogenase family, as evidenced by the domain organization and a distant homology (24% residue identity with the human class Iγ1 alcohol dehydrogenase).
Persson, Bengt +6 more
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Combining Xylose Reductase from Spathaspora arborariae with Xylitol Dehydrogenase from Spathaspora passalidarum to Promote Xylose Consumption and Fermentation into Xylitol by Saccharomyces cerevisiae [PDF]
In recent years, many novel xylose-fermenting yeasts belonging to the new genus Spathaspora have been isolated from the gut of wood-feeding insects and/or wood-decaying substrates.
Adriane Mouro +7 more
doaj +2 more sources
Abstract Due to its anticariogenic properties, the five-carbon sugar alcohol xylitol is finding increasing use in food industry as a functional sweetener. Although xylitol is currently manufactured by the chemical reduction of xylose, its biological production would be attractive because of lower costs and better organoleptic features.
TAMBURINI, Elena +3 more
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AbstractBACKGROUND: Xylitol is a sugar alcohol (polyalcohol) with many interesting properties for pharmaceutical and food products. It is currently produced by a chemical process, which has some disadvantages such as high energy requirement. Therefore microbiological production of xylitol has been studied as an alternative, but its viability is ...
Ricardo F Branco +5 more
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Inducible xylitol dehydrogenases in enteric bacteria [PDF]
Morganella morganii ATCC 25829, Providencia stuartii ATCC 25827, Serratia marcescens ATCC 13880, and Erwinia sp. strain 4D2P were found to induce a xylitol dehydrogenase when grown on a xylitol-containing medium. The xylitol dehydrogenases were partially purified from the four strains, and those from M. morganii ATCC 25829, P.
R C, Doten, R P, Mortlock
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Meyerozyma guilliermondii, a non-conventional yeast that naturally assimilates xylose, is considered as a candidate for biotechnological production of the sugar alternative xylitol. Because the genes of the xylose metabolism were yet unknown, all efforts
Denise Atzmüller +2 more
doaj +1 more source

