Results 41 to 50 of about 3,060 (171)

A comparative analysis of NADPH supply strategies in Saccharomyces cerevisiae: Production of d-xylitol from d-xylose as a case study

Metabolic Engineering Communications
Enhancing the supply of the redox cofactor NADPH in metabolically engineered cells is a critical target for optimizing the synthesis of many product classes, such as fatty acids or terpenoids. In S.
Priti Regmi, Melanie Knesebeck, Eckhard Boles, Dirk Weuster-Botz, Mislav Oreb   +4 more
doaj   +1 more source

Crystallization‐Based Purification of Xylitol Produced by Biotechnological Route

Chemical Engineering &Technology, Volume 49, Issue 4, April 2026.
This study investigated xylitol purification from fermentation broths using various crystallization techniques, including cooling, melt crystallization, and chromatographic pretreatment. Combining chromatographic separation with cooling crystallization achieved >99% xylitol purity, highlighting its potential for efficient industrial applications ...
Anna Zaykovskaya, Mitra Ila, Michael Ginter, Erik Temmel, Martin Schwiderski, Manfred Stepanski, Marjatta Louhi‐Kultanen   +6 more
wiley   +1 more source

Expression of xylitol dehydrogenase gene xyl2 in Saccharomyces cerevisiae [PDF]

AIP Conference Proceedings, 2018
Xylitol is oxidized to xylulose under the catalytic action of xylitol dehydrogenase, so xylitol dehydrogenase is at an important position in the metabolism of wood pond. The paper discusses the extraction, structure, pear flower properties and coenzyme modification of xylitol dehydrogenase, and studies the cloning and expression of xylitol ...
openaire   +1 more source

Comparison of the xylose reductase-xylitol dehydrogenase and the xylose isomerase pathways for xylose fermentation by recombinant Saccharomyces cerevisiae

Microbial Cell Factories, 2007
Background Two heterologous pathways have been used to construct recombinant xylose-fermenting Saccharomyces cerevisiae strains: i) the xylose reductase (XR) and xylitol dehydrogenase (XDH) pathway and ii) the xylose isomerase (XI) pathway.
Hahn-Hägerdal Bärbel, Sanchez Rosa, Karhumaa Kaisa, Gorwa-Grauslund Marie-F   +3 more
doaj   +1 more source

Lipid production from lignocellulosic biomass using an engineered Yarrowia lipolytica strain

Microbial Cell Factories, 2022
Background The utilization of industrial wastes as feedstock in microbial-based processes is a one of the high-potential approach for the development of sustainable, environmentally beneficial and valuable bioproduction, inter alia, lipids.
Katarzyna Drzymała-Kapinos, Aleksandra M. Mirończuk, Adam Dobrowolski   +2 more
doaj   +1 more source

Dynamic Shape Transformation of a DNA Scaffold Applied for an Enzyme Nanocarrier

Frontiers in Chemistry, 2021
Structural programmability and accurate addressability of DNA nanostructures are ideal characteristics for the platform of arranging enzymes with the nanoscale precision.
Peng Lin, Huyen Dinh, Eiji Nakata, Takashi Morii   +3 more
doaj   +1 more source

Report of the Scientific Committee of the Spanish Agency for Food Safety and Nutrition (AESAN) on Hereditary Fructose Intolerance (HFI), or aldolase B deficiency, and fructose malabsorption (intestinal fructose intolerance)

Food Risk Assess Europe, Volume 4, Issue 2, April 2026.
Abstract Hereditary Fructose Intolerance (HFI), also known as aldolase B deficiency, is an inherited metabolic disorder caused by the deficiency of that enzyme, which participates in the fructose metabolism in the liver, kidneys and small intestine. Aldolase B deficiency brings about the accumulation of fructose‐1‐phosphate in these organs, which can ...
Araceli Díaz Perales, Irene Bretón Lesmes, Ángel Gil Izquierdo, Gema Nieto Martínez, Silvia Pichardo Sánchez, María de Cortes Sánchez Mata, María Ángeles Carlos Chillerón   +6 more
wiley   +1 more source

Xylitol dehydrogenase fromPachysolen tannophilus

FEMS Microbiology Letters, 1984
NAD-xylitol-dehydrogenase (EC 1.1.1.9) from Pachysolen tannophilus was investigated in relation to xylitol byproduction during xylose fermentation by this yeast. For this purpose the enzyme was partially purified by a combination of affinity chromatography and fast liquid protein chromatography.
G. Ditzelmüller, C.P. Kubicek, W. Wöhrer, M. Röhr   +3 more
openaire   +1 more source

Oxygenation influences xylose fermentation and gene expression in the yeast genera Spathaspora and Scheffersomyces

Biotechnology for Biofuels and Bioproducts
Background Cost-effective production of biofuels from lignocellulose requires the fermentation of d-xylose. Many yeast species within and closely related to the genera Spathaspora and Scheffersomyces (both of the order Serinales) natively assimilate and ...
Katharina O. Barros, Megan Mader, David J. Krause, Jasmyn Pangilinan, Bill Andreopoulos, Anna Lipzen, Stephen J. Mondo, Igor V. Grigoriev, Carlos A. Rosa, Trey K. Sato, Chris Todd Hittinger   +10 more
doaj   +1 more source

Identification of Mutations Responsible for Improved Xylose Utilization in an Adapted Xylose Isomerase Expressing Saccharomyces cerevisiae Strain

Fermentation, 2022
Economic conversion of biomass to biofuels and chemicals requires efficient and complete utilization of xylose. Saccharomyces cerevisiae strains engineered for xylose utilization are still considerably limited in their overall ability to metabolize ...
Ronald E. Hector, Jeffrey A. Mertens, Nancy N. Nichols   +2 more
doaj   +1 more source