Results 51 to 60 of about 3,060 (171)

Systematic Metabolic Engineering and Model‐Guided Optimization for High‐Level Production of L‐Theanine from Xylose in Escherichia coli

open access: yesAdvanced Science, Volume 13, Issue 17, 23 March 2026.
This study is pioneering in constructing the shortest known synthetic pathway for L‐theanine production from xylose within E coli. Through comprehensive metabolic engineering strategies, our engineered strain achieved the highest reported L‐theanine titer from xylose, with a titer of 95.42 g/L, and a yield of 0.55 g/g.
Haolin Han   +5 more
wiley   +1 more source

Seasonal and Environmental Influences on Free Sugar and Amino Acid Profiles of Lycium barbarum Berries Cultivated in Southern Tuscany

open access: yesFood Science &Nutrition, Volume 14, Issue 2, February 2026.
The graphical abstract illustrates how temperature, rainfall, and ripening stage shape the metabolic profile of Lycium barbarum berries grown in Southern Tuscany. It highlights the optimal harvest window (S2–S4) and summarizes key trends in sugars (glucose, fructose, pectins) and amino acids (total FAAs, proline, BABA, ornithine). Visual cues show that
Letizia Poggioni   +4 more
wiley   +1 more source

Cloning and Expression of a Xylitol-4-Dehydrogenase Gene from Pantoea ananatis [PDF]

open access: yesApplied and Environmental Microbiology, 2006
ABSTRACT The Pantoea ananatis ATCC 43072 mutant strain is capable of growing with xylitol as the sole carbon source. The xylitol-4-dehydrogenase (XDH) catalyzing the oxidation of xylitol to l -xylulose was isolated from the cell extract of this strain.
J S, Aarnikunnas   +4 more
openaire   +2 more sources

Tailored GelMA‐Chitosan‐Polypyrrole‐ CNT Conductive Scaffold for Potential Application in Neural Tissue Regeneration

open access: yesMacromolecular Materials and Engineering, Volume 311, Issue 2, February 2026.
This study presents an optimal hydrogel that matches the physicochemical and biological properties of neural tissue. The presence of carbon nanotubes and polypyrrole in the GelMA‐chitosan matrix improved the biomechanical and bioelectric properties of the scaffold and increased the adhesion and viability of PC12 and SH‐SY5Y cells.
Pegah Madaninasab   +2 more
wiley   +1 more source

Polyol specificity of recombinant Arabidopsis thaliana sorbitol dehydrogenase studied by enzyme kinetics and in silico modeling

open access: yesFrontiers in Plant Science, 2015
Polyols are enzymatically-produced plant compounds which can act as compatible solutes during periods of abiotic stress. NAD+-dependent SORBITOL DEHYDROGENASE (SDH, E.C. 1.1.1.14) from Arabidopsis thaliana L.
María Francisca eAguayo   +6 more
doaj   +1 more source

Bioreduction of β‐ketosulfides Using Deep Eutectic Solvents as Cosolvents

open access: yesChemCatChem, Volume 18, Issue 2, 28 January 2026.
A set of β‐ketosulfides has been selectively reduced to optically active β‐hydroxysulfides employing KREDs as biocatalysts in the presence of Deep Eutectic Solvents. By appropriately selecting both the biocatalyst and the reaction medium, both alcohol enantiomers can be obtained in high yields and optical purities.
Chiara Falcini   +6 more
wiley   +1 more source

Xylose reductase from Pichia stipitis with altered coenzyme preference improves ethanolic xylose fermentation by recombinant Saccharomyces cerevisiae

open access: yesBiotechnology for Biofuels, 2009
Background Xylose reductase (XR) and xylitol dehydrogenase (XDH) from Pichia stipitis are the two enzymes most commonly used in recombinant Saccharomyces cerevisiae strains engineered for xylose utilization.
Hahn-Hägerdal Bärbel   +2 more
doaj   +1 more source

Potential of CRISPR/Cas9 Genome Engineering for Biofuel Production From Economically Relevant Algae

open access: yesInternational Journal of Energy Research, Volume 2026, Issue 1, 2026.
The worldwide energy demand continues to rise, while reliance on fossil fuels remains unsustainable due to global warming, declining reserves and rising oil prices. This has intensified the search for renewable alternatives. Among these, microscopic algae have emerged as a promising feedstock for third‐generation biofuels.
Sundram Kumari   +6 more
wiley   +1 more source

Comparative xylose metabolism among the Ascomycetes C. albicans, S. stipitis and S. cerevisiae.

open access: yesPLoS ONE, 2013
The ascomycetes Candida albicans, Saccharomyces cerevisiae and Scheffersomyces stipitis metabolize the pentose sugar xylose very differently. S. cerevisiae fails to grow on xylose, while C. albicans can grow, and S.
Doreen Harcus   +6 more
doaj   +1 more source

Altering the coenzyme preference of xylose reductase to favor utilization of NADH enhances ethanol yield from xylose in a metabolically engineered strain of Saccharomyces cerevisiae

open access: yesMicrobial Cell Factories, 2008
Background Metabolic engineering of Saccharomyces cerevisiae for xylose fermentation into fuel ethanol has oftentimes relied on insertion of a heterologous pathway that consists of xylose reductase (XR) and xylitol dehydrogenase (XDH) and brings about ...
Nidetzky Bernd, Petschacher Barbara
doaj   +1 more source

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